Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs

E.R. Earl; M.M. Waterston; E. Aughey; M.J. Harvey; C. Matschke; A. Colston; V.A. Ferro

doi:10.1016/j.vaccine.2006.01.041

Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs

E.R. Earl, M.M. Waterston, E. Aughey, M.J. Harvey, C. Matschke, A. Colston, V.A. Ferro

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid (TT) or diphtheria toxoid (DT) and formulated with Quil A saponin or a sustained release injectible PLGA (poly(lactide-co-glycolide)/triacetin). For the Quil A formulations, two administrations of TT conjugate at 3-weekly intervals were followed by two booster injections with the DT conjugate in entire ram lambs. With the PLGA formulations, only two injections were administered; the first containing TT and the second DT at 6-weekly intervals. Evaluation was carried out by comparing the specific antibody levels produced in relationship to hormone profiles and testicular changes. The Quil A formulation was considered the most effective, as it caused significant reduction in testosterone and follicle stimulating hormone levels, resulting in marked suppression of spermatogenesis.

Original language	English
Pages (from-to)	3172-3183
Number of pages	12
Journal	Vaccine
Volume	24
Issue number	16
Early online date	2 Feb 2006
DOIs	https://doi.org/10.1016/j.vaccine.2006.01.041
Publication status	Published - 12 Apr 2006

Keywords

GnRH
vaccine
anti-fertility

Access to Document

10.1016/j.vaccine.2006.01.041

http://dx.doi.org/10.1016/j.vaccine.2006.01.041

Cite this

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title = "Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs",

abstract = "A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid (TT) or diphtheria toxoid (DT) and formulated with Quil A saponin or a sustained release injectible PLGA (poly(lactide-co-glycolide)/triacetin). For the Quil A formulations, two administrations of TT conjugate at 3-weekly intervals were followed by two booster injections with the DT conjugate in entire ram lambs. With the PLGA formulations, only two injections were administered; the first containing TT and the second DT at 6-weekly intervals. Evaluation was carried out by comparing the specific antibody levels produced in relationship to hormone profiles and testicular changes. The Quil A formulation was considered the most effective, as it caused significant reduction in testosterone and follicle stimulating hormone levels, resulting in marked suppression of spermatogenesis. ",

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author = "E.R. Earl and M.M. Waterston and E. Aughey and M.J. Harvey and C. Matschke and A. Colston and V.A. Ferro",

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AU - Earl, E.R.

AU - Waterston, M.M.

AU - Aughey, E.

AU - Harvey, M.J.

AU - Matschke, C.

AU - Colston, A.

AU - Ferro, V.A.

PY - 2006/4/12

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AB - A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid (TT) or diphtheria toxoid (DT) and formulated with Quil A saponin or a sustained release injectible PLGA (poly(lactide-co-glycolide)/triacetin). For the Quil A formulations, two administrations of TT conjugate at 3-weekly intervals were followed by two booster injections with the DT conjugate in entire ram lambs. With the PLGA formulations, only two injections were administered; the first containing TT and the second DT at 6-weekly intervals. Evaluation was carried out by comparing the specific antibody levels produced in relationship to hormone profiles and testicular changes. The Quil A formulation was considered the most effective, as it caused significant reduction in testosterone and follicle stimulating hormone levels, resulting in marked suppression of spermatogenesis.

KW - GnRH

KW - vaccine

KW - anti-fertility

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