Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs

E.R. Earl, M.M. Waterston, E. Aughey, M.J. Harvey, C. Matschke, A. Colston, V.A. Ferro

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid (TT) or diphtheria toxoid (DT) and formulated with Quil A saponin or a sustained release injectible PLGA (poly(lactide-co-glycolide)/triacetin). For the Quil A formulations, two administrations of TT conjugate at 3-weekly intervals were followed by two booster injections with the DT conjugate in entire ram lambs. With the PLGA formulations, only two injections were administered; the first containing TT and the second DT at 6-weekly intervals. Evaluation was carried out by comparing the specific antibody levels produced in relationship to hormone profiles and testicular changes. The Quil A formulation was considered the most effective, as it caused significant reduction in testosterone and follicle stimulating hormone levels, resulting in marked suppression of spermatogenesis.

LanguageEnglish
Pages3172-3183
Number of pages12
JournalVaccine
Volume24
Issue number16
Early online date2 Feb 2006
DOIs
Publication statusPublished - 12 Apr 2006

Fingerprint

Diphtheria Toxoid
Suffolk (sheep breed)
Tetanus Toxoid
toxoids
Gonadotropin-Releasing Hormone
rams
Testis
testes
lambs
Vaccines
vaccines
tetanus
Triacetin
Testicular Hormones
Polyglactin 910
Injections
Saponins
Follicle Stimulating Hormone
Spermatogenesis
Testosterone

Keywords

  • GnRH
  • vaccine
  • anti-fertility

Cite this

Earl, E.R. ; Waterston, M.M. ; Aughey, E. ; Harvey, M.J. ; Matschke, C. ; Colston, A. ; Ferro, V.A. / Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs. In: Vaccine. 2006 ; Vol. 24, No. 16. pp. 3172-3183.
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Earl, ER, Waterston, MM, Aughey, E, Harvey, MJ, Matschke, C, Colston, A & Ferro, VA 2006, 'Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs' Vaccine, vol. 24, no. 16, pp. 3172-3183. https://doi.org/10.1016/j.vaccine.2006.01.041

Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs. / Earl, E.R.; Waterston, M.M.; Aughey, E.; Harvey, M.J.; Matschke, C.; Colston, A.; Ferro, V.A.

In: Vaccine, Vol. 24, No. 16, 12.04.2006, p. 3172-3183.

Research output: Contribution to journalArticle

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Earl ER, Waterston MM, Aughey E, Harvey MJ, Matschke C, Colston A et al. Evaluation of two GnRH-I based vaccine formulations on the testes function of entire suffolk cross ram lambs. Vaccine. 2006 Apr 12;24(16):3172-3183. https://doi.org/10.1016/j.vaccine.2006.01.041