Abstract
A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid (TT) or diphtheria toxoid (DT) and formulated with Quil A saponin or a sustained release injectible PLGA (poly(lactide-co-glycolide)/triacetin). For the Quil A formulations, two administrations of TT conjugate at 3-weekly intervals were followed by two booster injections with the DT conjugate in entire ram lambs. With the PLGA formulations, only two injections were administered; the first containing TT and the second DT at 6-weekly intervals. Evaluation was carried out by comparing the specific antibody levels produced in relationship to hormone profiles and testicular changes. The Quil A formulation was considered the most effective, as it caused significant reduction in testosterone and follicle stimulating hormone levels, resulting in marked suppression of spermatogenesis.
Original language | English |
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Pages (from-to) | 3172-3183 |
Number of pages | 12 |
Journal | Vaccine |
Volume | 24 |
Issue number | 16 |
Early online date | 2 Feb 2006 |
DOIs | |
Publication status | Published - 12 Apr 2006 |
Keywords
- GnRH
- vaccine
- anti-fertility