Evaluation of the immunodiagnostic potential of a recombinant surface protein domain from Acanthamoeba castellanii

Alemao G.Carpinteyro Sánchez, Veridiana Gomes Virginio, Vinicius José Maschio, Henrique Bunselmeyer Ferreira, Marilise Brittes Rott

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Acanthamoeba spp. are free-living protists widely distributed in environment, able to cause keratitis, encephalitis and skin lesions in humans and animals. Acanthamoeba spp. exist in two forms: an infective trophozoite and a dormant cyst. Several factors contribute to the pathogenesis of Acanthamoeba spp. The parasite adhesion to the host cell is the primary step for infection and is mediated by a mannose binding-protein, expressed in the surface and considered the main pathogenicity factor in Acanthamoeba spp. So far, there was no evidence of another surface protein of Acanthamoeba spp. relevant for host invasion or infection by these organisms. The aims of this study were to identify and characterize an Acanthamoeba castellanii surface protein and to evaluate its diagnostic potential. In silico predictions of surface proteins allowed to identify the A. castellanii calreticulin as a possible surface antigen. The coding sequence of a predicted extracellular domain of A. castellanii calreticulin was cloned by in vivo homologous recombination and the recombinant polypeptide (AcCRT29-130) was produced. Its immunodiagnostic potential was assessed in a recombinant antigen-based ELISA with sera from experimentally infected rats that developed keratitis and encephalitis, and sera from patients with encephalitis. The AcCRT29-130 was significantly more recognized by sera from encephalitis infected rats in comparison with the non-infected controls. Human sera from encephalitis patients, however presented no significant response. These results showed the AcCRT29-130 potential for A. castellanii infection immunodiagnosis in animals, with further studies being required for assessment of its use for human infections.

LanguageEnglish
Pages1656-1664
Number of pages9
JournalParasitology
Volume143
Issue number12
DOIs
Publication statusPublished - 1 Oct 2016

Fingerprint

Acanthamoeba castellanii
Acanthamoeba
surface proteins
Encephalitis
encephalitis
Recombinant Proteins
recombinant proteins
Membrane Proteins
calreticulin
Calreticulin
keratitis
Keratitis
Infection
Serum
infection
Mannose-Binding Lectin
recombinant antigens
Trophozoites
Immunologic Tests
trophozoites

Keywords

  • Acanthamoeba castellanii
  • calreticulin
  • ELISA
  • immunodiagnostic
  • recombinant protein

Cite this

Sánchez, Alemao G.Carpinteyro ; Virginio, Veridiana Gomes ; Maschio, Vinicius José ; Ferreira, Henrique Bunselmeyer ; Rott, Marilise Brittes. / Evaluation of the immunodiagnostic potential of a recombinant surface protein domain from Acanthamoeba castellanii. In: Parasitology. 2016 ; Vol. 143, No. 12. pp. 1656-1664.
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Evaluation of the immunodiagnostic potential of a recombinant surface protein domain from Acanthamoeba castellanii. / Sánchez, Alemao G.Carpinteyro; Virginio, Veridiana Gomes; Maschio, Vinicius José; Ferreira, Henrique Bunselmeyer; Rott, Marilise Brittes.

In: Parasitology, Vol. 143, No. 12, 01.10.2016, p. 1656-1664.

Research output: Contribution to journalArticle

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AU - Sánchez, Alemao G.Carpinteyro

AU - Virginio, Veridiana Gomes

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