Evaluation of nerve cell toxicity in vitro by electrophysiological and biochemical methods

K Xie, A L Harvey

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The acute toxicity of the first nine standard chemicals in the Multicentre Evaluation of In Vitro Cytotoxicity Tests (MEIC) programme was evaluated in the NG108-15 neuroblastoma cell line by monitoring changes in cell resting membrane potential (RMP) and by using the MTT assay to measure cell viability. Cells were differentiated with dibutyryl-cAMP and then exposed to different concentrations of the chemicals for 1 hr or 24 hr. At each concentration and time point, RMPs were measured from about 30 differentiated cells and MTT assays were performed on parallel cultures. IC(50) values were obtained from linear regression analysis. The results showed that the IC(50)s from MTT assays correlated closely with those from RMP measurements (r = 0.983 for 1 hr exposure; r = 0.933 for 24 hr exposure). IC(50)s of amitriptyline and diazepam were 0.1-1.9 mm; alcoholic compounds (isopropanol, ethylene glycol, ethanol and methanol) had IC(50)s from 121.5 mm to 3731.9 mm; paracetamol, aspirin and ferrous sulphate had intermediate cytotoxicity (IC(50) 2.6-53.5 mm). IC(50)s decreased markedly with increased exposure time. RMP is expected to be a sensitive indicator of the health of nerve cells; however, its measurement in a large number of cells is laborious. MTT assays are rapid, and the close correlation between IC(50)s in the two types of assay suggests that MTT assays could be used to evaluate cytotoxicity in neuronal cells in vitro.
Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalToxicology in Vitro
Volume7
Issue number3
DOIs
Publication statusPublished - May 1993

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Membrane Potentials
Neurons
Toxicity
Assays
ferrous sulfate
Cytotoxicity
Amitriptyline
Ethylene Glycol
2-Propanol
Diazepam
Neuroblastoma
Aspirin
Methanol
Linear Models
Cell Survival
Ethanol
Cell Count
Cells
Regression Analysis
Cell Membrane

Keywords

  • cyto-toxicity
  • assay
  • cultures
  • dehydrogenase
  • MEIC list
  • 10 chemicals

Cite this

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Evaluation of nerve cell toxicity in vitro by electrophysiological and biochemical methods. / Xie, K; Harvey, A L.

In: Toxicology in Vitro, Vol. 7, No. 3, 05.1993, p. 275-279.

Research output: Contribution to journalArticle

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