Evaluation of 30 insertion/deletion polymorphisms as forensic markers in the Kuwaiti population

Mahdi Haidar, Hussain Alsaleh, Penelope R. Haddrill

Research output: Contribution to journal › Article

Abstract

Insertion/deletion (indel) polymorphism markers have been developed as a supplementary genotyping system in forensic DNA testing. The only commercial indel genotyping kit is the Qiagen Investigator® DIPplex kit, which includes 30 loci that can be typed using PCR-capillary electrophoresis (PCR-CE). We show that these 30 markers can be used to genetically assign Kuwaiti individuals to their population of origin when compared to other distant populations, using principal components analysis (PCA) and STRUCTURE. We also demonstrate a relationship between geographic and genetic distance using allele frequencies for the 30 markers from 26 populations.
Original languageEnglish
JournalForensic Science International: Genetics Supplement Series
Early online date21 Oct 2019
DOIs
Publication statusE-pub ahead of print - 21 Oct 2019

Fingerprint

genetic polymorphism
genotyping
Population
capillary electrophoresis
Capillary Electrophoresis
Principal Component Analysis
Gene Frequency
gene frequency
genetic distance
principal component analysis
Research Personnel
Polymerase Chain Reaction
loci
DNA
forensic sciences
testing

Keywords

  • kuwait
  • indels
  • DIPplex
  • forensic genetics

Cite this

Haidar, Mahdi ; Alsaleh, Hussain ; Haddrill, Penelope R. / Evaluation of 30 insertion/deletion polymorphisms as forensic markers in the Kuwaiti population. In: Forensic Science International: Genetics Supplement Series. 2019.
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Haidar, M, Alsaleh, H & Haddrill, PR 2019, 'Evaluation of 30 insertion/deletion polymorphisms as forensic markers in the Kuwaiti population', Forensic Science International: Genetics Supplement Series. https://doi.org/10.1016/j.fsigss.2019.10.157

Evaluation of 30 insertion/deletion polymorphisms as forensic markers in the Kuwaiti population. / Haidar, Mahdi; Alsaleh, Hussain; Haddrill, Penelope R.

In: Forensic Science International: Genetics Supplement Series, 21.10.2019.

Research output: Contribution to journal › Article

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AU - Alsaleh, Hussain

AU - Haddrill, Penelope R.

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Y1 - 2019/10/21

N2 - Insertion/deletion (indel) polymorphism markers have been developed as a supplementary genotyping system in forensic DNA testing. The only commercial indel genotyping kit is the Qiagen Investigator® DIPplex kit, which includes 30 loci that can be typed using PCR-capillary electrophoresis (PCR-CE). We show that these 30 markers can be used to genetically assign Kuwaiti individuals to their population of origin when compared to other distant populations, using principal components analysis (PCA) and STRUCTURE. We also demonstrate a relationship between geographic and genetic distance using allele frequencies for the 30 markers from 26 populations.

AB - Insertion/deletion (indel) polymorphism markers have been developed as a supplementary genotyping system in forensic DNA testing. The only commercial indel genotyping kit is the Qiagen Investigator® DIPplex kit, which includes 30 loci that can be typed using PCR-capillary electrophoresis (PCR-CE). We show that these 30 markers can be used to genetically assign Kuwaiti individuals to their population of origin when compared to other distant populations, using principal components analysis (PCA) and STRUCTURE. We also demonstrate a relationship between geographic and genetic distance using allele frequencies for the 30 markers from 26 populations.

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Haidar M, Alsaleh H, Haddrill PR. Evaluation of 30 insertion/deletion polymorphisms as forensic markers in the Kuwaiti population. Forensic Science International: Genetics Supplement Series. 2019 Oct 21. https://doi.org/10.1016/j.fsigss.2019.10.157

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    Embargo ends: 21/10/20