European propolis is highly active against trypanosomatids including Crithidia fasciculata

Abdullah Alotaibi, Godwin U. Ebiloma, Roderick Williams, Samya Alenezi, Anne-Marie Donachie, Selome Guillaume, John O. Igoli, James Fearnley, Harry P. de Koning, David G. Watson

Research output: Contribution to journalArticle

Abstract

Extracts of 35 samples of European propolis were tested against wild type and resistant strains of the protozoal pathogens Trypanosoma brucei, Trypanosoma congolense and Leishmania mexicana. The extracts were also tested against Crithidia fasciculata a close relative of Crithidia mellificae, a parasite of bees. Crithidia, Trypanosoma and Leishmania are all members of the order Kinetoplastida. High levels of activity were obtained for all the samples with the levels of activity varying across the sample set. The highest levels of activity were found against L. mexicana. The propolis samples were profiled by using liquid chromatography with high resolution mass spectrometry (LC-MS) and principal components analysis (PCA) of the data obtained indicated there was a wide variation in the composition of the propolis samples. Orthogonal partial least squares (OPLS) associated a butyrate ester of pinobanksin with high activity against T. brucei whereas in the case of T. congolense high activity was associated with methyl ethers of chrysin and pinobanksin. In the case of C. fasciculata highest activity was associated with methyl ethers of galangin and pinobanksin. OPLS modelling of the activities against L. mexicana using the mass spectrometry produced a less successful model suggesting a wider range of active components.

LanguageEnglish
Article number11364
Number of pages10
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 6 Aug 2019

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Crithidia fasciculata
Leishmania mexicana
Propolis
Crithidia
Trypanosoma congolense
Methyl Ethers
Trypanosoma brucei brucei
Least-Squares Analysis
Mass Spectrometry
Kinetoplastida
Trypanosoma
Butyrates
Bees
Leishmania
Principal Component Analysis
Liquid Chromatography
Esters
Parasites
pinobanksin

Keywords

  • propolis
  • mass spectrometry
  • trypanosomatid parasites

Cite this

Alotaibi, Abdullah ; Ebiloma, Godwin U. ; Williams, Roderick ; Alenezi, Samya ; Donachie, Anne-Marie ; Guillaume, Selome ; Igoli, John O. ; Fearnley, James ; de Koning, Harry P. ; Watson, David G. / European propolis is highly active against trypanosomatids including Crithidia fasciculata. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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Alotaibi, A, Ebiloma, GU, Williams, R, Alenezi, S, Donachie, A-M, Guillaume, S, Igoli, JO, Fearnley, J, de Koning, HP & Watson, DG 2019, 'European propolis is highly active against trypanosomatids including Crithidia fasciculata' Scientific Reports, vol. 9, no. 1, 11364. https://doi.org/10.1038/s41598-019-47840-y

European propolis is highly active against trypanosomatids including Crithidia fasciculata. / Alotaibi, Abdullah; Ebiloma, Godwin U.; Williams, Roderick; Alenezi, Samya; Donachie, Anne-Marie; Guillaume, Selome; Igoli, John O.; Fearnley, James; de Koning, Harry P.; Watson, David G.

In: Scientific Reports, Vol. 9, No. 1, 11364, 06.08.2019.

Research output: Contribution to journalArticle

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AU - Alotaibi, Abdullah

AU - Ebiloma, Godwin U.

AU - Williams, Roderick

AU - Alenezi, Samya

AU - Donachie, Anne-Marie

AU - Guillaume, Selome

AU - Igoli, John O.

AU - Fearnley, James

AU - de Koning, Harry P.

AU - Watson, David G.

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