Estrogen metabolites in a small cohort of patients with idiopathic pulmonary arterial hypertension

Nina Denver, Natalie Z.M. Homer, Ruth Andrew, Katie Y. Harvey, Nicholas Morrell, Eric D. Austin, Margaret R. MacLean

Research output: Contribution to journalArticle

1 Downloads (Pure)

Abstract

Increased risk and severity of idiopathic pulmonary arterial hypertension (iPAH) is associated with elevated plasma estradiol (E2) in both men and postmenopausal women, suggesting that E2 is not ovarian-derived. Aromatase is responsible for the synthesis of E2. Remodelled pulmonary arteries, lesions and human pulmonary artery smooth muscle cells (hPASMCs) from PAH patients have high levels of aromatase. CYP1B1 can convert E2 to mitogenic 16-hydroxy-estrogens (16OHEs) and this enzyme is over-expressed in diseased pulmonary arteries from PAH patients. Existing assays have not been sensitive or specific enough to detect levels of many E2 metabolites in PAH patient blood. Using a newly developed liquid chromatography tandem mass spectrometry (LC-MS/MS) assay, here we investigate the profile of several E2 metabolites in iPAH patients.
Original languageEnglish
Number of pages5
JournalPulmonary Circulation
Volume10
Issue number1
Early online date13 Feb 2020
DOIs
Publication statusE-pub ahead of print - 13 Feb 2020

    Fingerprint

Keywords

  • idiopathic pulmonary arterial hypertension (iPAH)
  • plasma estradiol (E2)
  • postmenopausal women
  • estrogen

Cite this

Denver, N., Homer, N. Z. M., Andrew, R., Harvey, K. Y., Morrell, N., Austin, E. D., & MacLean, M. R. (2020). Estrogen metabolites in a small cohort of patients with idiopathic pulmonary arterial hypertension. Pulmonary Circulation, 10(1). https://doi.org/10.1177/2045894020908783