Estimation of fluorescence lifetimes via rotational invariance techniques

Hongqi Yu, Rebecca Saleeb, Paul Dalgarno, David Day-Uei Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Estimation of signal parameters via rotational invariance techniques is a classical algorithm widely used in array signal processing for direction-of-arrival estimation of emitters. Inspired by this method, a new signal model and a new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multi-exponential fluorescence lifetime imaging (FLIM) experiments. The FLERIT only requires a few time bins of a histogram generated by a time-correlated single photon counting FLIM system, greatly reducing the data throughput from the imager to the signal processing units. As a non-iterative method, the FLERIT does not require initial conditions, prior information nor model selection that are usually required by widely used traditional fitting methods, including nonlinear least square methods or maximum likelihood methods. Moreover, its simplicity means it is suitable for implementations in embedded systems for real-time applications. FLERIT was tested on synthesized and experimental fluorescent cell data showing the potentials to be widely applied in FLIM data analysis.
LanguageEnglish
Pages1292-1300
Number of pages9
JournalIEEE Transactions on Biomedical Engineering
Volume63
Issue number6
Early online date25 Nov 2015
DOIs
Publication statusPublished - 1 Jun 2016

Fingerprint

Invariance
Fluorescence
Optical Imaging
Signal processing
Computer Systems
Least-Squares Analysis
Photons
Imaging techniques
Direction of arrival
Bins
Image sensors
Embedded systems
Imaging systems
Maximum likelihood
Throughput
Experiments

Keywords

  • fluorescence microscopy
  • fluorescence lifetime imaging microscopy
  • time correlated single photon counting
  • time resolved imaging

Cite this

Yu, Hongqi ; Saleeb, Rebecca ; Dalgarno, Paul ; Li, David Day-Uei. / Estimation of fluorescence lifetimes via rotational invariance techniques. In: IEEE Transactions on Biomedical Engineering. 2016 ; Vol. 63, No. 6. pp. 1292-1300.
@article{68c6865e455b43dfb078d6495b51c451,
title = "Estimation of fluorescence lifetimes via rotational invariance techniques",
abstract = "Estimation of signal parameters via rotational invariance techniques is a classical algorithm widely used in array signal processing for direction-of-arrival estimation of emitters. Inspired by this method, a new signal model and a new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multi-exponential fluorescence lifetime imaging (FLIM) experiments. The FLERIT only requires a few time bins of a histogram generated by a time-correlated single photon counting FLIM system, greatly reducing the data throughput from the imager to the signal processing units. As a non-iterative method, the FLERIT does not require initial conditions, prior information nor model selection that are usually required by widely used traditional fitting methods, including nonlinear least square methods or maximum likelihood methods. Moreover, its simplicity means it is suitable for implementations in embedded systems for real-time applications. FLERIT was tested on synthesized and experimental fluorescent cell data showing the potentials to be widely applied in FLIM data analysis.",
keywords = "fluorescence microscopy, fluorescence lifetime imaging microscopy, time correlated single photon counting, time resolved imaging",
author = "Hongqi Yu and Rebecca Saleeb and Paul Dalgarno and Li, {David Day-Uei}",
note = "(c) 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.",
year = "2016",
month = "6",
day = "1",
doi = "10.1109/TBME.2015.2491364",
language = "English",
volume = "63",
pages = "1292--1300",
journal = "IEEE Transactions on Biomedical Engineering",
issn = "0018-9294",
number = "6",

}

Estimation of fluorescence lifetimes via rotational invariance techniques. / Yu, Hongqi; Saleeb, Rebecca; Dalgarno, Paul; Li, David Day-Uei.

In: IEEE Transactions on Biomedical Engineering, Vol. 63, No. 6, 01.06.2016, p. 1292-1300.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Estimation of fluorescence lifetimes via rotational invariance techniques

AU - Yu, Hongqi

AU - Saleeb, Rebecca

AU - Dalgarno, Paul

AU - Li, David Day-Uei

N1 - (c) 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Estimation of signal parameters via rotational invariance techniques is a classical algorithm widely used in array signal processing for direction-of-arrival estimation of emitters. Inspired by this method, a new signal model and a new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multi-exponential fluorescence lifetime imaging (FLIM) experiments. The FLERIT only requires a few time bins of a histogram generated by a time-correlated single photon counting FLIM system, greatly reducing the data throughput from the imager to the signal processing units. As a non-iterative method, the FLERIT does not require initial conditions, prior information nor model selection that are usually required by widely used traditional fitting methods, including nonlinear least square methods or maximum likelihood methods. Moreover, its simplicity means it is suitable for implementations in embedded systems for real-time applications. FLERIT was tested on synthesized and experimental fluorescent cell data showing the potentials to be widely applied in FLIM data analysis.

AB - Estimation of signal parameters via rotational invariance techniques is a classical algorithm widely used in array signal processing for direction-of-arrival estimation of emitters. Inspired by this method, a new signal model and a new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multi-exponential fluorescence lifetime imaging (FLIM) experiments. The FLERIT only requires a few time bins of a histogram generated by a time-correlated single photon counting FLIM system, greatly reducing the data throughput from the imager to the signal processing units. As a non-iterative method, the FLERIT does not require initial conditions, prior information nor model selection that are usually required by widely used traditional fitting methods, including nonlinear least square methods or maximum likelihood methods. Moreover, its simplicity means it is suitable for implementations in embedded systems for real-time applications. FLERIT was tested on synthesized and experimental fluorescent cell data showing the potentials to be widely applied in FLIM data analysis.

KW - fluorescence microscopy

KW - fluorescence lifetime imaging microscopy

KW - time correlated single photon counting

KW - time resolved imaging

UR - http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=10

U2 - 10.1109/TBME.2015.2491364

DO - 10.1109/TBME.2015.2491364

M3 - Article

VL - 63

SP - 1292

EP - 1300

JO - IEEE Transactions on Biomedical Engineering

T2 - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

SN - 0018-9294

IS - 6

ER -