Estimating microzooplankton grazing half-saturation constants from dilution experiments with nonlinear feeding kinetics

Bingzhang Chen, Edward A. Laws, Hongbin Liu, Bangqin Huang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To obtain the grazing half-saturation constant (K) of natural microzooplankton assemblages, we used three nonlinear grazing models (rectilinear, Holling type II, and Holling type III) to fit the detailed data (phytoplankton net specific growth rate vs. dilution factor) of individual dilution experiments that show significant concave curves. In the dataset consisting of 528 experiments, 96 experiments show significant concave curves, and the associated chlorophyll a (Chl a) concentrations are significantly higher than those of experiments showing linear or convex curves. Experiments showing concave curves likely reflect that these microzooplankton assemblages were under top-down control. The three models perform equally well in fitting the data. The K values estimated from these 96 experiments vary over three orders of magnitude and are log–log linearly related with ambient Chl a concentrations, but not correlated with temperatures. Estimates of K of natural microzooplankton assemblages tend to be smaller than estimates from laboratory cultures. For the experiments not showing concave curves, it is hard to obtain a robust estimate of K.
LanguageEnglish
Pages639-644
Number of pages6
JournalLimnology and Oceanography
Volume59
Issue number3
DOIs
Publication statusPublished - 7 Apr 2014

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dilution
grazing
saturation
chlorophyll
kinetics
specific growth rate
experiment
phytoplankton
chlorophyll a
temperature
top-down control

Keywords

  • microzooplankton
  • dilution experiments
  • nonlinear feeding kinetics
  • grazing half-saturation constan
  • nonlinear grazing models

Cite this

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abstract = "To obtain the grazing half-saturation constant (K) of natural microzooplankton assemblages, we used three nonlinear grazing models (rectilinear, Holling type II, and Holling type III) to fit the detailed data (phytoplankton net specific growth rate vs. dilution factor) of individual dilution experiments that show significant concave curves. In the dataset consisting of 528 experiments, 96 experiments show significant concave curves, and the associated chlorophyll a (Chl a) concentrations are significantly higher than those of experiments showing linear or convex curves. Experiments showing concave curves likely reflect that these microzooplankton assemblages were under top-down control. The three models perform equally well in fitting the data. The K values estimated from these 96 experiments vary over three orders of magnitude and are log–log linearly related with ambient Chl a concentrations, but not correlated with temperatures. Estimates of K of natural microzooplankton assemblages tend to be smaller than estimates from laboratory cultures. For the experiments not showing concave curves, it is hard to obtain a robust estimate of K.",
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Estimating microzooplankton grazing half-saturation constants from dilution experiments with nonlinear feeding kinetics. / Chen, Bingzhang; Laws, Edward A.; Liu, Hongbin; Huang, Bangqin.

In: Limnology and Oceanography , Vol. 59, No. 3, 07.04.2014, p. 639-644.

Research output: Contribution to journalArticle

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AB - To obtain the grazing half-saturation constant (K) of natural microzooplankton assemblages, we used three nonlinear grazing models (rectilinear, Holling type II, and Holling type III) to fit the detailed data (phytoplankton net specific growth rate vs. dilution factor) of individual dilution experiments that show significant concave curves. In the dataset consisting of 528 experiments, 96 experiments show significant concave curves, and the associated chlorophyll a (Chl a) concentrations are significantly higher than those of experiments showing linear or convex curves. Experiments showing concave curves likely reflect that these microzooplankton assemblages were under top-down control. The three models perform equally well in fitting the data. The K values estimated from these 96 experiments vary over three orders of magnitude and are log–log linearly related with ambient Chl a concentrations, but not correlated with temperatures. Estimates of K of natural microzooplankton assemblages tend to be smaller than estimates from laboratory cultures. For the experiments not showing concave curves, it is hard to obtain a robust estimate of K.

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