Establishing the mitochondrial DNA D-loop structure of Columba livia

L.C. Tsai, J.C.I. Lee, S.P. Liao, L.H. Weng, A.M.T. Linacre, H.M. Hsieh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The complete mitochondrial DNA D-loop structure of pigeon (Columba livia) was established in this study. A strategy of amplifying three partial fragments of the D-loop and then combing the three fragments to cover the full length of the D-loop was adopted. Ten samples from pigeons were collected and were successfully amplified and sequenced. Repetitive sequences of a VNTR and an STR were both observed at the 3-end of D-loop region. DNA sequence data revealed polymorphic sequences including indels, SNP, VNTR and STR within the D-loop. The size of the D-loop ranged from 1310 to 1327 bp from the initiation site of D-loop to the site immediately upstream of the repeat sequences depending upon the number of insertions or deletions. Each sample could be distinguished based on four genotyping procedures; being indels, SNPs, VNTRs and STRs. The polymorphic nature of the D-loop can be a valuable method for maternal identification and genetic linkage of pigeon in particular forensic science investigations.
LanguageEnglish
Pages3058-3062
Number of pages4
JournalElectrophoresis
Volume30
Issue number17
DOIs
Publication statusPublished - Sep 2009

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Nucleic Acid Repetitive Sequences
DNA sequences
Columbidae
Mitochondrial DNA
Single Nucleotide Polymorphism
Forensic Sciences
Genetic Linkage
Mothers
Forensic science

Keywords

  • mitochondrial DNA D-loop
  • pigeon
  • SNP
  • STR
  • VNTR

Cite this

Tsai, L. C., Lee, J. C. I., Liao, S. P., Weng, L. H., Linacre, A. M. T., & Hsieh, H. M. (2009). Establishing the mitochondrial DNA D-loop structure of Columba livia. Electrophoresis, 30(17), 3058-3062. https://doi.org/10.1002/elps.200900194
Tsai, L.C. ; Lee, J.C.I. ; Liao, S.P. ; Weng, L.H. ; Linacre, A.M.T. ; Hsieh, H.M. / Establishing the mitochondrial DNA D-loop structure of Columba livia. In: Electrophoresis. 2009 ; Vol. 30, No. 17. pp. 3058-3062.
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Tsai, LC, Lee, JCI, Liao, SP, Weng, LH, Linacre, AMT & Hsieh, HM 2009, 'Establishing the mitochondrial DNA D-loop structure of Columba livia' Electrophoresis, vol. 30, no. 17, pp. 3058-3062. https://doi.org/10.1002/elps.200900194

Establishing the mitochondrial DNA D-loop structure of Columba livia. / Tsai, L.C.; Lee, J.C.I.; Liao, S.P.; Weng, L.H.; Linacre, A.M.T.; Hsieh, H.M.

In: Electrophoresis, Vol. 30, No. 17, 09.2009, p. 3058-3062.

Research output: Contribution to journalArticle

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Tsai LC, Lee JCI, Liao SP, Weng LH, Linacre AMT, Hsieh HM. Establishing the mitochondrial DNA D-loop structure of Columba livia. Electrophoresis. 2009 Sep;30(17):3058-3062. https://doi.org/10.1002/elps.200900194