Establishing the mitochondrial DNA D-loop structure of Columba livia

L.C. Tsai, J.C.I. Lee, S.P. Liao, L.H. Weng, A.M.T. Linacre, H.M. Hsieh

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Abstract

The complete mitochondrial DNA D-loop structure of pigeon (Columba livia) was established in this study. A strategy of amplifying three partial fragments of the D-loop and then combing the three fragments to cover the full length of the D-loop was adopted. Ten samples from pigeons were collected and were successfully amplified and sequenced. Repetitive sequences of a VNTR and an STR were both observed at the 3-end of D-loop region. DNA sequence data revealed polymorphic sequences including indels, SNP, VNTR and STR within the D-loop. The size of the D-loop ranged from 1310 to 1327 bp from the initiation site of D-loop to the site immediately upstream of the repeat sequences depending upon the number of insertions or deletions. Each sample could be distinguished based on four genotyping procedures; being indels, SNPs, VNTRs and STRs. The polymorphic nature of the D-loop can be a valuable method for maternal identification and genetic linkage of pigeon in particular forensic science investigations.
Original languageEnglish
Pages (from-to)3058-3062
Number of pages4
JournalElectrophoresis
Volume30
Issue number17
DOIs
Publication statusPublished - Sep 2009

Keywords

  • mitochondrial DNA D-loop
  • pigeon
  • SNP
  • STR
  • VNTR

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    Tsai, L. C., Lee, J. C. I., Liao, S. P., Weng, L. H., Linacre, A. M. T., & Hsieh, H. M. (2009). Establishing the mitochondrial DNA D-loop structure of Columba livia. Electrophoresis, 30(17), 3058-3062. https://doi.org/10.1002/elps.200900194