Projects per year
Abstract
We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.
Original language | English |
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Article number | 141 |
Number of pages | 12 |
Journal | Biosensors |
Volume | 9 |
Issue number | 4 |
DOIs | |
Publication status | Published - 6 Dec 2019 |
Keywords
- field-effect transistor
- biosensor
- TP53
- electrochemistry
- open-circuit potential
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Dive into the research topics of 'Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53): an electrochemical optimisation approach'. Together they form a unique fingerprint.Projects
- 1 Finished
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EPSRC Centre for Doctoral Training in Medical Devices and Health Technologies
Connolly, P. (Principal Investigator), Black, R. A. (Co-investigator), Conway, B. A. (Co-investigator), Graham, D. (Co-investigator), Hunter, I. (Co-investigator), Mathieson, K. (Co-investigator), Ulijn, R. (Co-investigator) & Winn, P. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/04/14 → 30/09/22
Project: Research - Studentship
Datasets
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Data for: "Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach"
Hannah, S. (Creator), Corrigan, D. (Creator), Crossley, L. (Creator), ATTOYE, B. (Creator), Vezza, V. J. (Creator) & Blair, E. (Creator), University of Strathclyde, 9 Dec 2019
DOI: 10.15129/216a65df-3b6e-4e93-9c2d-dfc8cae1eb7b
Dataset