Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53): an electrochemical optimisation approach

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Abstract

We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.
Original languageEnglish
Article number141
Number of pages12
JournalBiosensors
Volume9
Issue number4
DOIs
Publication statusPublished - 6 Dec 2019

Keywords

  • field-effect transistor
  • biosensor
  • TP53
  • electrochemistry
  • open-circuit potential

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