Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53)

an electrochemical optimisation approach

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Abstract

We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.
Original languageEnglish
Article number141
Number of pages12
JournalBiosensors
Volume9
Issue number4
DOIs
Publication statusPublished - 6 Dec 2019

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Field effect transistors
Tumors
DNA
Genes
Proteins
Mutation
Sensors
Neoplasms
Potentiometry
Costs and Cost Analysis
Gold
Electrodes
Costs
Transistors
Networks (circuits)
Electric potential

Keywords

  • field-effect transistor
  • biosensor
  • TP53
  • electrochemistry
  • open-circuit potential

Cite this

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abstract = "We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.",
keywords = "field-effect transistor, biosensor, TP53, electrochemistry, open-circuit potential",
author = "Lisa Crossley and Bukola Attoye and Vincent Vezza and Ewen Blair and Corrigan, {Damion K.} and Stuart Hannah",
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AU - Blair, Ewen

AU - Corrigan, Damion K.

AU - Hannah, Stuart

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N2 - We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.

AB - We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.

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KW - electrochemistry

KW - open-circuit potential

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