Projects per year
We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.
- field-effect transistor
- open-circuit potential
1/04/14 → 30/09/22
Project: Research - Studentship
Data for: "Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach"
Crossley, L., Attoye, B., Vezza, V., Blair, E., Corrigan, D. K., & Hannah, S. (2019). Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53): an electrochemical optimisation approach. Biosensors, 9(4), . https://doi.org/10.3390/bios9040141