Erythrocyte glutathione balance and membrane stability during preeclampsia

C.M. Spickett, J. Reglinski, W.E. Smith, R. Wilson, J.J. Walker, J. McKillop

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

This study aimed to determine whether oxidative damage to the erythrocyte occurs in preeclampsia, and relates to disease severity. The oxidative status of intact erythrocytes from preeclamptic patients and normal pregnant women was determined using spin echo 1H-NMR, which measures both the concentration and redox state of intracellular glutathione. Previous studies of preeclampsia have only measured total glutathione levels. Membrane fragility was determined from the degree of lysis caused by incubation in hypotonic saline. Erythrocytes from moderate-severe preeclamptic patients underwent more lysis than erythrocytes from control pregnant women (p < .05) or mild preeclamptic patients. It is suggested that increased lysis results from oxidative damage to the erythrocyte membrane, causing a decrease in membrane fluidity and reducing its ability to withstand osmotic changes. Intracellular glutathione was more oxidized in erythrocytes from pregnant women compared to nonpregnant controls (p < .05), and there was a less significant trend toward more oxidized glutathione with increasing severity of preeclampsia. The moderate-severe group showed a clear division in glutathione redox status: some patients had very oxidized glutathione while others had a normal redox balance. This novel finding suggests that some patients may be unusually susceptible to erythrocyte glutathione oxidation, possibly leading to general cellular damage, in particular HELLP Syndrome.
Original languageEnglish
Pages (from-to)1049-1055
Number of pages6
JournalFree Radical Biology and Medicine
Volume24
Issue number6
DOIs
Publication statusPublished - Apr 1998

Fingerprint

Pre-Eclampsia
Glutathione
Erythrocytes
Membranes
Glutathione Disulfide
Oxidation-Reduction
Pregnant Women
HELLP Syndrome
Fluidity
Membrane Fluidity
Erythrocyte Membrane
Nuclear magnetic resonance
Oxidation

Keywords

  • oxidative stress
  • glutathione balance
  • preeclampsia
  • membrane fragility
  • erythrocyte
  • H-1 spin echo NMR
  • free radical

Cite this

Spickett, C.M. ; Reglinski, J. ; Smith, W.E. ; Wilson, R. ; Walker, J.J. ; McKillop, J. / Erythrocyte glutathione balance and membrane stability during preeclampsia. In: Free Radical Biology and Medicine. 1998 ; Vol. 24, No. 6. pp. 1049-1055.
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Erythrocyte glutathione balance and membrane stability during preeclampsia. / Spickett, C.M.; Reglinski, J.; Smith, W.E.; Wilson, R.; Walker, J.J.; McKillop, J.

In: Free Radical Biology and Medicine, Vol. 24, No. 6, 04.1998, p. 1049-1055.

Research output: Contribution to journalArticle

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