Enzyme hydrolysis of cassava peels: treatment by amylolytic and cellulolytic enzymes

John Ikuba Ona, Peter J. Halling, Mercedes Ballesteros

Research output: Contribution to journalArticle

Abstract

Cassava peels provide a cheap non-food biomass waste that can be hydrolyzed to simple sugars as a useful feedstock. Unlike most crop wastes, they have high starch content as well as lignocellulose. In this study, an enzymatic treatment of cassava peels by various concentrations of amylase and glucoamylase is considered. Steam explosion pre-treatments reduced rate and yield of hydrolysis. Milled peels suspended at 10% w/v yielded a maximum reducing sugar of 0.41 g (as glucose) per gram of peels. HPLC analysis showed that levels of soluble oligosaccharides remained low throughout. A pretreatment with amylase at 95 °C slightly increased rates although final yield was the same. Additional treatment with cellulolytic enzymes increases the total hydrolysis yield to 0.61 g (as glucose) per gram of peels representing 91% of the carbohydrate in cassava peels.

LanguageEnglish
Pages77-85
Number of pages9
JournalBiocatalysis and Biotransformation
Volume37
Issue number2
Early online date11 Jan 2019
DOIs
Publication statusPublished - 4 Mar 2019

Fingerprint

Manihot
Amylases
Sugars
Glucose
Hydrolysis
Enzymes
Glucan 1,4-alpha-Glucosidase
Oligosaccharides
Steam
Carbohydrates
Starch
Feedstocks
Crops
Explosions
Biomass
High Pressure Liquid Chromatography
lignocellulose

Keywords

  • cassava peel
  • biomass waste
  • feedstock
  • crop waste
  • cellulolytic hydrolysis
  • starch rich biomass

Cite this

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abstract = "Cassava peels provide a cheap non-food biomass waste that can be hydrolyzed to simple sugars as a useful feedstock. Unlike most crop wastes, they have high starch content as well as lignocellulose. In this study, an enzymatic treatment of cassava peels by various concentrations of amylase and glucoamylase is considered. Steam explosion pre-treatments reduced rate and yield of hydrolysis. Milled peels suspended at 10{\%} w/v yielded a maximum reducing sugar of 0.41 g (as glucose) per gram of peels. HPLC analysis showed that levels of soluble oligosaccharides remained low throughout. A pretreatment with amylase at 95 °C slightly increased rates although final yield was the same. Additional treatment with cellulolytic enzymes increases the total hydrolysis yield to 0.61 g (as glucose) per gram of peels representing 91{\%} of the carbohydrate in cassava peels.",
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Enzyme hydrolysis of cassava peels : treatment by amylolytic and cellulolytic enzymes. / Ona, John Ikuba; Halling, Peter J.; Ballesteros, Mercedes.

In: Biocatalysis and Biotransformation, Vol. 37, No. 2, 04.03.2019, p. 77-85.

Research output: Contribution to journalArticle

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AU - Halling, Peter J.

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