Enzyme-activated RGD ligands on functionalized poly(ethylene glycol) monolayers: surface analysis and cellular response

S.J. Todd, D.J. Scurr, J.E. Gough, M.R. Alexander, R.V. Ulijn

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We report on the design, stepwise synthesis, and surface analysis of enzyme-responsive surfaces that present cell adhesive RGD sequences on-demand, that is, by enzymatic hydrolysis of inactive RGD containing precursors that carry cleavable steric blocking groups. These surfaces, incorporating poly(ethylene glycol) (PEG) monolayers coupled via epoxy silanes to glass, are functionalized via stepwise solid phase synthesis, presenting a versatile and straightforward approach to preparation of peptide surfaces. Successive amino acid coupling and deprotection steps using fluorenylmethoxycarbonyl (Fmoc) chemistry are verified using surface analysis with time-of-flight secondary-ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS). Exposure of surfaces to elastase results in activation of cell binding ligands as demonstrated using osteoblast cells. These surfaces may have applications in spatiotemporally controlled attachment of cells as relevant for three-dimensional tissue engineering scaffolds and cell-based biosensors.
LanguageEnglish
Pages7533-7539
Number of pages6
JournalLangmuir
Volume25
Issue number13
DOIs
Publication statusPublished - 30 Apr 2009

Fingerprint

Surface analysis
Polyethylene glycols
enzymes
glycols
Monolayers
ethylene
Enzymes
Ligands
ligands
cells
Tissue Scaffolds
Silanes
Enzymatic hydrolysis
Pancreatic Elastase
Osteoblasts
Scaffolds (biology)
Secondary ion mass spectrometry
Tissue engineering
Biosensors
Peptides

Keywords

  • enzyme-responsive surfaces
  • enzymatic hydrolysis
  • solid phase synthesis
  • mass spectrometry
  • x-ray photoelectron spectroscopy

Cite this

Todd, S.J. ; Scurr, D.J. ; Gough, J.E. ; Alexander, M.R. ; Ulijn, R.V. / Enzyme-activated RGD ligands on functionalized poly(ethylene glycol) monolayers: surface analysis and cellular response. In: Langmuir. 2009 ; Vol. 25, No. 13. pp. 7533-7539.
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Enzyme-activated RGD ligands on functionalized poly(ethylene glycol) monolayers: surface analysis and cellular response. / Todd, S.J.; Scurr, D.J.; Gough, J.E.; Alexander, M.R.; Ulijn, R.V.

In: Langmuir, Vol. 25, No. 13, 30.04.2009, p. 7533-7539.

Research output: Contribution to journalArticle

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