Environmental scanning electron microscope imaging of vesicle systems

Yvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U. R. Mohammed, Sarah E. McNeil, Anil Vangala

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The structural characteristics of liposomes have been widely investigated and there is certainly a strong understanding of their morphological characteristics. Imaging of these systems, using techniques such as freeze-fracturing methods, transmission electron microscopy, and cryo-electron imaging, has allowed us to appreciate their bilayer structures and factors which can influence this. However, there are few methods which all us to study these systems in their natural hydrated state; commonly the liposomes are visualized after drying, staining, and/or fixation of the vesicles. Environmental Scanning Electron Microscopy (ESEM) offers the ability to image a liposome in its hydrated state without the need for prior sample preparation. Within our studies we were the first to use ESEM to study liposomes and niosomes and we have been able to dynamically follow the hydration of lipid films and changes in liposome suspensions as water condenses on to, or evaporates from, the sample in real time. This provides insight into the resistance of liposomes to coalescence during dehydration, thereby providing an alternative assay of liposome formulation and stability.

LanguageEnglish
Title of host publicationMethods in Molecular Biology
EditorsGerard G.M. D'Souza
Place of PublicationNew York
Pages131-143
Number of pages13
DOIs
Publication statusPublished - 12 Nov 2016

Publication series

NameMethods in Molecular Biology
Volume1522
ISSN (Print)10643745

Fingerprint

Liposomes
Electrons
Electron Scanning Microscopy
Freeze Fracturing
Transmission Electron Microscopy
Dehydration
Suspensions
Staining and Labeling
Lipids
Water

Keywords

  • ESEM analysis
  • lipoplexes
  • liposomes
  • niosomes
  • non-ionic surfactant vesicles
  • surfactant vesicles

Cite this

Perrie, Y., Ali, H., Kirby, D. J., Mohammed, A. U. R., McNeil, S. E., & Vangala, A. (2016). Environmental scanning electron microscope imaging of vesicle systems. In G. G. M. D'Souza (Ed.), Methods in Molecular Biology (pp. 131-143). (Methods in Molecular Biology; Vol. 1522). New York. https://doi.org/10.1007/978-1-4939-6591-5_11
Perrie, Yvonne ; Ali, Habib ; Kirby, Daniel J. ; Mohammed, Afzal U. R. ; McNeil, Sarah E. ; Vangala, Anil. / Environmental scanning electron microscope imaging of vesicle systems. Methods in Molecular Biology. editor / Gerard G.M. D'Souza. New York, 2016. pp. 131-143 (Methods in Molecular Biology).
@inbook{6ac5f5b96bf54e5b9435af54db11b9f3,
title = "Environmental scanning electron microscope imaging of vesicle systems",
abstract = "The structural characteristics of liposomes have been widely investigated and there is certainly a strong understanding of their morphological characteristics. Imaging of these systems, using techniques such as freeze-fracturing methods, transmission electron microscopy, and cryo-electron imaging, has allowed us to appreciate their bilayer structures and factors which can influence this. However, there are few methods which all us to study these systems in their natural hydrated state; commonly the liposomes are visualized after drying, staining, and/or fixation of the vesicles. Environmental Scanning Electron Microscopy (ESEM) offers the ability to image a liposome in its hydrated state without the need for prior sample preparation. Within our studies we were the first to use ESEM to study liposomes and niosomes and we have been able to dynamically follow the hydration of lipid films and changes in liposome suspensions as water condenses on to, or evaporates from, the sample in real time. This provides insight into the resistance of liposomes to coalescence during dehydration, thereby providing an alternative assay of liposome formulation and stability.",
keywords = "ESEM analysis, lipoplexes, liposomes, niosomes, non-ionic surfactant vesicles, surfactant vesicles",
author = "Yvonne Perrie and Habib Ali and Kirby, {Daniel J.} and Mohammed, {Afzal U. R.} and McNeil, {Sarah E.} and Anil Vangala",
year = "2016",
month = "11",
day = "12",
doi = "10.1007/978-1-4939-6591-5_11",
language = "English",
isbn = "978-1-4939-6589-2",
series = "Methods in Molecular Biology",
pages = "131--143",
editor = "D'Souza, {Gerard G.M.}",
booktitle = "Methods in Molecular Biology",

}

Perrie, Y, Ali, H, Kirby, DJ, Mohammed, AUR, McNeil, SE & Vangala, A 2016, Environmental scanning electron microscope imaging of vesicle systems. in GGM D'Souza (ed.), Methods in Molecular Biology. Methods in Molecular Biology, vol. 1522, New York, pp. 131-143. https://doi.org/10.1007/978-1-4939-6591-5_11

Environmental scanning electron microscope imaging of vesicle systems. / Perrie, Yvonne; Ali, Habib; Kirby, Daniel J.; Mohammed, Afzal U. R.; McNeil, Sarah E.; Vangala, Anil.

Methods in Molecular Biology. ed. / Gerard G.M. D'Souza. New York, 2016. p. 131-143 (Methods in Molecular Biology; Vol. 1522).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Environmental scanning electron microscope imaging of vesicle systems

AU - Perrie, Yvonne

AU - Ali, Habib

AU - Kirby, Daniel J.

AU - Mohammed, Afzal U. R.

AU - McNeil, Sarah E.

AU - Vangala, Anil

PY - 2016/11/12

Y1 - 2016/11/12

N2 - The structural characteristics of liposomes have been widely investigated and there is certainly a strong understanding of their morphological characteristics. Imaging of these systems, using techniques such as freeze-fracturing methods, transmission electron microscopy, and cryo-electron imaging, has allowed us to appreciate their bilayer structures and factors which can influence this. However, there are few methods which all us to study these systems in their natural hydrated state; commonly the liposomes are visualized after drying, staining, and/or fixation of the vesicles. Environmental Scanning Electron Microscopy (ESEM) offers the ability to image a liposome in its hydrated state without the need for prior sample preparation. Within our studies we were the first to use ESEM to study liposomes and niosomes and we have been able to dynamically follow the hydration of lipid films and changes in liposome suspensions as water condenses on to, or evaporates from, the sample in real time. This provides insight into the resistance of liposomes to coalescence during dehydration, thereby providing an alternative assay of liposome formulation and stability.

AB - The structural characteristics of liposomes have been widely investigated and there is certainly a strong understanding of their morphological characteristics. Imaging of these systems, using techniques such as freeze-fracturing methods, transmission electron microscopy, and cryo-electron imaging, has allowed us to appreciate their bilayer structures and factors which can influence this. However, there are few methods which all us to study these systems in their natural hydrated state; commonly the liposomes are visualized after drying, staining, and/or fixation of the vesicles. Environmental Scanning Electron Microscopy (ESEM) offers the ability to image a liposome in its hydrated state without the need for prior sample preparation. Within our studies we were the first to use ESEM to study liposomes and niosomes and we have been able to dynamically follow the hydration of lipid films and changes in liposome suspensions as water condenses on to, or evaporates from, the sample in real time. This provides insight into the resistance of liposomes to coalescence during dehydration, thereby providing an alternative assay of liposome formulation and stability.

KW - ESEM analysis

KW - lipoplexes

KW - liposomes

KW - niosomes

KW - non-ionic surfactant vesicles

KW - surfactant vesicles

UR - http://www.scopus.com/inward/record.url?scp=84995527572&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6591-5_11

DO - 10.1007/978-1-4939-6591-5_11

M3 - Chapter

SN - 978-1-4939-6589-2

T3 - Methods in Molecular Biology

SP - 131

EP - 143

BT - Methods in Molecular Biology

A2 - D'Souza, Gerard G.M.

CY - New York

ER -

Perrie Y, Ali H, Kirby DJ, Mohammed AUR, McNeil SE, Vangala A. Environmental scanning electron microscope imaging of vesicle systems. In D'Souza GGM, editor, Methods in Molecular Biology. New York. 2016. p. 131-143. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6591-5_11