Environmental scanning electron microscope imaging of vesicle systems

Yvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U. R. Mohammed, Sarah E. McNeil, Anil Vangala

Research output: Contribution to journalArticle

Abstract

The structural characteristics of liposomes have been widely investigated and there is certainly a strong understanding of their morphological characteristics. Imaging of these systems, using techniques such as freeze-fracturing methods, transmission electron microscopy, and cryo-electron imaging, has allowed us to appreciate their bilayer structures and factors that influence this. However, there are a few methods that study these systems in their natural hydrated state; commonly, the liposomes are visualized after drying, staining and/or fixation of the vesicles. Environmental scanning electron microscopy (ESEM) offers the ability to image a liposome in its hydrated state without the need for prior sample preparation. We were the first to use ESEM to study the liposomes and niosomes, and have been able to dynamically follow the hydration of lipid films and changes in liposome suspensions as water condenses onto, or evaporates from, the sample in real-time. This provides an insight into the resistance of liposomes to coalescence during dehydration, thereby providing an alternative assay for liposome formulation and stability.

LanguageEnglish
Pages319-331
Number of pages13
JournalMethods in Molecular Biology
Volume606
DOIs
Publication statusPublished - 23 Nov 2009

Fingerprint

Liposomes
Electrons
Electron Scanning Microscopy
Freeze Fracturing
Transmission Electron Microscopy
Dehydration
Suspensions
Staining and Labeling
Lipids
Water

Keywords

  • lipids
  • liposomes
  • microscopy, electron, scanning
  • surface-active agents

Cite this

Perrie, Yvonne ; Ali, Habib ; Kirby, Daniel J. ; Mohammed, Afzal U. R. ; McNeil, Sarah E. ; Vangala, Anil. / Environmental scanning electron microscope imaging of vesicle systems. In: Methods in Molecular Biology. 2009 ; Vol. 606. pp. 319-331.
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Environmental scanning electron microscope imaging of vesicle systems. / Perrie, Yvonne; Ali, Habib; Kirby, Daniel J.; Mohammed, Afzal U. R.; McNeil, Sarah E.; Vangala, Anil.

In: Methods in Molecular Biology, Vol. 606, 23.11.2009, p. 319-331.

Research output: Contribution to journalArticle

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