Entrapment of plasmid DNA vaccines into liposomes by dehydration/rehydration

Gregory Gregoriadis, Brenda McCormack, Mia Obrenovich, Yvonne Perrie

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Intramuscular injection of naked plasmid DNA is known (1–3) to elicit humoral and cell-mediated immune responses against the encoded antigen. It is thought (2,3) that immunity follows DNA uptake by muscle cells, leading to the expression and extracellular release of the antigen which is then taken up by antigen presenting cells (APC). In addition, it is feasible that some of the injected DNA is taken up directly by APC. Disadvantages (1–3) of naked DNA vaccination include: uptake of DNA by only a minor fraction of muscle cells, exposure of DNA to deoxyribonuclease in the interstitial fluid thus necessitating the use of relatively large quantities of DNA, and, in some cases, injection into regenerating muscle in order to enhance immunity. We have recently proposed (1,4) that DNA immunization via liposomes (phospholipid vesicles) could circumvent the need of muscle involvement and instead facilitate (5) uptake of DNA by APC infiltrating the site of injection or in the lymphatics, at the same time protecting DNA from nuclease attack (6). Moreover, transfection of APC with liposomal DNA could be promoted by the judicial choice of vesicle surface charge, size and lipid composition, or by the co-entrapment, together with DNA, of plasmids expressing appropriate cytokines (e.g., interleukin 2), or immunostimulatory sequences.
LanguageEnglish
Title of host publicationDNA Vaccines
Subtitle of host publicationMethods and Protocols
EditorsDouglas B. Lowrie, Robert G. Whalen
Place of PublicationTotowa, NJ.
Pages305-311
Number of pages7
DOIs
Publication statusPublished - 23 Sep 1999

Publication series

NameMethods in molecular medicine
PublisherHumana Press
Volume29
ISSN (Print)1543-1894

Fingerprint

DNA Vaccines
Fluid Therapy
Dehydration
Liposomes
Plasmids
DNA
Antigen-Presenting Cells
Deoxyribonucleases
Muscle Cells
Immunity
Antigens
Muscles
Injections
Intramuscular Injections
Extracellular Fluid
Interleukin-2
Transfection
Immunization
Phospholipids
Vaccination

Keywords

  • chemical manufacturing
  • pharmacy

Cite this

Gregoriadis, G., McCormack, B., Obrenovich, M., & Perrie, Y. (1999). Entrapment of plasmid DNA vaccines into liposomes by dehydration/rehydration. In D. B. Lowrie, & R. G. Whalen (Eds.), DNA Vaccines: Methods and Protocols (pp. 305-311). (Methods in molecular medicine; Vol. 29). Totowa, NJ.. https://doi.org/10.1385/1-59259-688-6:305
Gregoriadis, Gregory ; McCormack, Brenda ; Obrenovich, Mia ; Perrie, Yvonne. / Entrapment of plasmid DNA vaccines into liposomes by dehydration/rehydration. DNA Vaccines: Methods and Protocols. editor / Douglas B. Lowrie ; Robert G. Whalen. Totowa, NJ., 1999. pp. 305-311 (Methods in molecular medicine).
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Gregoriadis, G, McCormack, B, Obrenovich, M & Perrie, Y 1999, Entrapment of plasmid DNA vaccines into liposomes by dehydration/rehydration. in DB Lowrie & RG Whalen (eds), DNA Vaccines: Methods and Protocols. Methods in molecular medicine, vol. 29, Totowa, NJ., pp. 305-311. https://doi.org/10.1385/1-59259-688-6:305

Entrapment of plasmid DNA vaccines into liposomes by dehydration/rehydration. / Gregoriadis, Gregory; McCormack, Brenda; Obrenovich, Mia; Perrie, Yvonne.

DNA Vaccines: Methods and Protocols. ed. / Douglas B. Lowrie; Robert G. Whalen. Totowa, NJ., 1999. p. 305-311 (Methods in molecular medicine; Vol. 29).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

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N2 - Intramuscular injection of naked plasmid DNA is known (1–3) to elicit humoral and cell-mediated immune responses against the encoded antigen. It is thought (2,3) that immunity follows DNA uptake by muscle cells, leading to the expression and extracellular release of the antigen which is then taken up by antigen presenting cells (APC). In addition, it is feasible that some of the injected DNA is taken up directly by APC. Disadvantages (1–3) of naked DNA vaccination include: uptake of DNA by only a minor fraction of muscle cells, exposure of DNA to deoxyribonuclease in the interstitial fluid thus necessitating the use of relatively large quantities of DNA, and, in some cases, injection into regenerating muscle in order to enhance immunity. We have recently proposed (1,4) that DNA immunization via liposomes (phospholipid vesicles) could circumvent the need of muscle involvement and instead facilitate (5) uptake of DNA by APC infiltrating the site of injection or in the lymphatics, at the same time protecting DNA from nuclease attack (6). Moreover, transfection of APC with liposomal DNA could be promoted by the judicial choice of vesicle surface charge, size and lipid composition, or by the co-entrapment, together with DNA, of plasmids expressing appropriate cytokines (e.g., interleukin 2), or immunostimulatory sequences.

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EP - 311

BT - DNA Vaccines

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CY - Totowa, NJ.

ER -

Gregoriadis G, McCormack B, Obrenovich M, Perrie Y. Entrapment of plasmid DNA vaccines into liposomes by dehydration/rehydration. In Lowrie DB, Whalen RG, editors, DNA Vaccines: Methods and Protocols. Totowa, NJ. 1999. p. 305-311. (Methods in molecular medicine). https://doi.org/10.1385/1-59259-688-6:305