Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

M.S.L. Raddatz, A. Dolf, E. Endl, P. Knolle, M. Famulok, G. Mayer

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.
LanguageEnglish
Pages5190-5193
Number of pages3
JournalAgewandte Chemie-International Edition
Volume47
Issue number28
DOIs
Publication statusPublished - Jun 2008

Fingerprint

Sorting
Nucleic acids
Cells
Nucleic Acids
Libraries
SELEX Aptamer Technique
Population
Burkitt Lymphoma
Ligands
Composite materials

Keywords

  • aptamers
  • c ell recognition
  • flow cytometry
  • fluorescence
  • in vitro selection

Cite this

Raddatz, M.S.L. ; Dolf, A. ; Endl, E. ; Knolle, P. ; Famulok, M. ; Mayer, G. / Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. In: Agewandte Chemie-International Edition. 2008 ; Vol. 47, No. 28. pp. 5190-5193.
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Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. / Raddatz, M.S.L.; Dolf, A.; Endl, E.; Knolle, P.; Famulok, M.; Mayer, G.

In: Agewandte Chemie-International Edition, Vol. 47, No. 28, 06.2008, p. 5190-5193.

Research output: Contribution to journalArticle

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AU - Famulok, M.

AU - Mayer, G.

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KW - flow cytometry

KW - fluorescence

KW - in vitro selection

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M3 - Article

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