Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

M.S.L. Raddatz, A. Dolf, E. Endl, P. Knolle, M. Famulok, G. Mayer

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129 Citations (Scopus)

Abstract

In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.
Original languageEnglish
Pages (from-to)5190-5193
Number of pages3
JournalAgewandte Chemie-International Edition
Volume47
Issue number28
DOIs
Publication statusPublished - Jun 2008

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Keywords

  • aptamers
  • c ell recognition
  • flow cytometry
  • fluorescence
  • in vitro selection

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