Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

M.S.L. Raddatz; A. Dolf; E. Endl; P. Knolle; M. Famulok; G. Mayer

doi:10.1002/anie.200800216

Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

M.S.L. Raddatz, A. Dolf, E. Endl, P. Knolle, M. Famulok, G. Mayer

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

160 Citations (Scopus)

Abstract

In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.

Original language	English
Pages (from-to)	5190-5193
Number of pages	3
Journal	Agewandte Chemie-International Edition
Volume	47
Issue number	28
DOIs	https://doi.org/10.1002/anie.200800216
Publication status	Published - Jun 2008

Keywords

aptamers
c ell recognition
flow cytometry
fluorescence
in vitro selection

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10.1002/anie.200800216

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abstract = "In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.",

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AU - Endl, E.

AU - Knolle, P.

AU - Famulok, M.

AU - Mayer, G.

PY - 2008/6

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AB - In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.

KW - aptamers

KW - c ell recognition

KW - flow cytometry

KW - fluorescence

KW - in vitro selection

UR - http://dx.doi.org/10.1002/anie.200800216

U2 - 10.1002/anie.200800216

DO - 10.1002/anie.200800216

M3 - Article

SN - 1433-7851

VL - 47

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JO - Agewandte Chemie-International Edition

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IS - 28

ER -

Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

Abstract

Keywords

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