In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.
- c ell recognition
- flow cytometry
- in vitro selection
Raddatz, M. S. L., Dolf, A., Endl, E., Knolle, P., Famulok, M., & Mayer, G. (2008). Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. Agewandte Chemie-International Edition, 47(28), 5190-5193. https://doi.org/10.1002/anie.200800216