Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays

M. Ito, F. Nakamura, A. Baba, K. Tamada, H. Ushijima, K. H. A. Lau, A. Manna, W. Knoll

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have developed a new methodology to fabricate high-resolution DNA microarrays (2500 dots/cm(2)) in combination with microcontact printing (mu CP) and surface plasmon resonance (SPR) imaging. A novel COOH-terminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically bound to a gold substrate for surface coupling reactions with NH2-terminated DNA. The hybridization between target DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfully demonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. The gold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles (similar to 10%) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancement effect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectric constants and Fresnel's equation.
LanguageEnglish
Pages11653-11662
Number of pages10
JournalJournal of Physical Chemistry C
Volume111
Issue number31
Early online date19 Jul 2007
DOIs
Publication statusPublished - 2007

Fingerprint

Surface plasmon resonance
Microarrays
surface plasmon resonance
Gold
DNA
deoxyribonucleic acid
gold
Nanoparticles
nanoparticles
augmentation
Immobilized Nucleic Acids
carborane
Imaging techniques
DNA Probes
disulfides
Substrates
initiators
printing
Disulfides
Polyethylene glycols

Keywords

  • raman-scattering
  • self-assembled
  • monolayers
  • quartz-crystal
  • microbalance
  • fluorescence
  • spectroscopy
  • thin-films
  • imaging
  • measurements
  • photoisomerization
  • reaction
  • optical
  • characterization
  • oligonucleotide
  • arrays
  • materials
  • science

Cite this

Ito, M. ; Nakamura, F. ; Baba, A. ; Tamada, K. ; Ushijima, H. ; Lau, K. H. A. ; Manna, A. ; Knoll, W. / Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays. In: Journal of Physical Chemistry C. 2007 ; Vol. 111, No. 31. pp. 11653-11662.
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abstract = "We have developed a new methodology to fabricate high-resolution DNA microarrays (2500 dots/cm(2)) in combination with microcontact printing (mu CP) and surface plasmon resonance (SPR) imaging. A novel COOH-terminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically bound to a gold substrate for surface coupling reactions with NH2-terminated DNA. The hybridization between target DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfully demonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. The gold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles (similar to 10{\%}) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancement effect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectric constants and Fresnel's equation.",
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Ito, M, Nakamura, F, Baba, A, Tamada, K, Ushijima, H, Lau, KHA, Manna, A & Knoll, W 2007, 'Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays' Journal of Physical Chemistry C, vol. 111, no. 31, pp. 11653-11662. https://doi.org/10.1021/jp070524m

Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays. / Ito, M.; Nakamura, F.; Baba, A.; Tamada, K.; Ushijima, H.; Lau, K. H. A.; Manna, A.; Knoll, W.

In: Journal of Physical Chemistry C, Vol. 111, No. 31, 2007, p. 11653-11662.

Research output: Contribution to journalArticle

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AU - Ito, M.

AU - Nakamura, F.

AU - Baba, A.

AU - Tamada, K.

AU - Ushijima, H.

AU - Lau, K. H. A.

AU - Manna, A.

AU - Knoll, W.

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AB - We have developed a new methodology to fabricate high-resolution DNA microarrays (2500 dots/cm(2)) in combination with microcontact printing (mu CP) and surface plasmon resonance (SPR) imaging. A novel COOH-terminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically bound to a gold substrate for surface coupling reactions with NH2-terminated DNA. The hybridization between target DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfully demonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. The gold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles (similar to 10%) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancement effect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectric constants and Fresnel's equation.

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