Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polypropylenimine polyplex

Hibah Mobarak Aldawsari, Ruangelie Edrada-Ebel, David Blatchford, Rothwelle Tate, Laurence Tetley, Christine Dufès

Research output: Contribution to journalArticle

39 Citations (Scopus)
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Abstract

The possibility of using non-viral gene delivery systems for the treatment of cancer is currently limited by their lower transfection efficacy compared to viral systems. On the basis that amino acids such as arginine, lysine and leucine were involved in enhancing DNA transportation into cells, we hypothesized that the grafting of these amino acids to the highly promising generation 3 diaminobutyric polypropylenimine (DAB) dendrimer would improve its transfection efficacy in cancer cells. In this work we demonstrated that the conjugation of arginine, lysine and leucine to the dendrimer led to an enhanced anti-proliferative activity of the polyplexes, by up to 47-fold for DAB-Lys in T98G cancer cells compared to the unmodified polyplex in vitro. In vivo, the intravenous administration of amino acid-bearing DAB polyplexes resulted in a significantly improved tumor gene expression, with the highest gene expression level observed after treatment with DAB-Lys polyplex. Arginine, lysine and leucine-bearing generation 3 polypropylenimine polymers are therefore highly promising gene delivery systems for gene transfection in tumors.

Original languageEnglish
Pages (from-to)5889-5899
Number of pages11
JournalBiomaterials
Volume32
Issue number25
Early online date18 May 2011
DOIs
Publication statusPublished - Sep 2011

Keywords

  • amino acid
  • gene expression
  • dendrimer
  • DNA
  • nanoparticles

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