Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polyethylenimine polyplex

Hibah Mobarak Aldawsari, Behin Sundara Raj, Ruangelie Edrada-Ebel, David Blatchford, Rothwelle Tate, Laurence Tetley, Christine Dufès

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The potential of gene therapy to treat cancer is currently limited by the low expression of therapeutic genes in the tumors. As amino acids are known to have excellent properties in cell penetration and gene expression regulation, we investigated if the conjugation of arginine, lysine and leucine onto the surface of the gene delivery system polyethylenimine could lead to an improved gene expression in tumors. The intravenous administration of arginine-, lysine- and Leucine-bearing polyethylenimine polyplexes led to a significant increase of gene expression in the tumor, with a β-galactosidase expression amount at least 3-fold higher than that obtained after treatment with unmodified polyethylenimine polyplex. The three amino acid-bearing polyethylenimine led to similar levels of gene expression in the tumor. The treatments were well tolerated by the mice. Arginine-, lysine- and leucine-bearing polyethylenimine are therefore highly promising gene delivery systems for cancer therapy.
Original languageEnglish
Pages (from-to)815-823
Number of pages9
JournalNanomedicine: Nanotechnology, Biology and Medicine
Volume7
Issue number5
Early online date17 Feb 2011
DOIs
Publication statusPublished - 2011

Keywords

  • gene delivery
  • amino acid
  • polyethylenimine
  • cancer therapy
  • gene expression

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