Abstract
| Language | English |
|---|---|
| Pages | 815-823 |
| Number of pages | 9 |
| Journal | Nanomedicine: Nanotechnology, Biology and Medicine |
| Volume | 7 |
| Issue number | 5 |
| Early online date | 17 Feb 2011 |
| DOIs | |
| State | Published - 2011 |
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Keywords
- gene delivery
- amino acid
- polyethylenimine
- cancer therapy
- gene expression
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Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polyethylenimine polyplex. / Aldawsari, Hibah Mobarak; Sundara Raj, Behin; Edrada-Ebel, Ruangelie; Blatchford, David; Tate, Rothwelle; Tetley, Laurence; Dufès, Christine.
In: Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 7, No. 5, 2011, p. 815-823.Research output: Contribution to journal › Article
TY - JOUR
T1 - Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polyethylenimine polyplex
AU - Aldawsari,Hibah Mobarak
AU - Sundara Raj,Behin
AU - Edrada-Ebel,Ruangelie
AU - Blatchford,David
AU - Tate,Rothwelle
AU - Tetley,Laurence
AU - Dufès,Christine
PY - 2011
Y1 - 2011
N2 - The potential of gene therapy to treat cancer is currently limited by the low expression of therapeutic genes in the tumors. As amino acids are known to have excellent properties in cell penetration and gene expression regulation, we investigated if the conjugation of arginine, lysine and leucine onto the surface of the gene delivery system polyethylenimine could lead to an improved gene expression in tumors. The intravenous administration of arginine-, lysine- and Leucine-bearing polyethylenimine polyplexes led to a significant increase of gene expression in the tumor, with a β-galactosidase expression amount at least 3-fold higher than that obtained after treatment with unmodified polyethylenimine polyplex. The three amino acid-bearing polyethylenimine led to similar levels of gene expression in the tumor. The treatments were well tolerated by the mice. Arginine-, lysine- and leucine-bearing polyethylenimine are therefore highly promising gene delivery systems for cancer therapy.
AB - The potential of gene therapy to treat cancer is currently limited by the low expression of therapeutic genes in the tumors. As amino acids are known to have excellent properties in cell penetration and gene expression regulation, we investigated if the conjugation of arginine, lysine and leucine onto the surface of the gene delivery system polyethylenimine could lead to an improved gene expression in tumors. The intravenous administration of arginine-, lysine- and Leucine-bearing polyethylenimine polyplexes led to a significant increase of gene expression in the tumor, with a β-galactosidase expression amount at least 3-fold higher than that obtained after treatment with unmodified polyethylenimine polyplex. The three amino acid-bearing polyethylenimine led to similar levels of gene expression in the tumor. The treatments were well tolerated by the mice. Arginine-, lysine- and leucine-bearing polyethylenimine are therefore highly promising gene delivery systems for cancer therapy.
KW - gene delivery
KW - amino acid
KW - polyethylenimine
KW - cancer therapy
KW - gene expression
U2 - 10.1016/j.nano.2011.01.016
DO - 10.1016/j.nano.2011.01.016
M3 - Article
VL - 7
SP - 815
EP - 823
JO - Nanomedicine: Nanotechnology, Biology and Medicine
T2 - Nanomedicine: Nanotechnology, Biology and Medicine
JF - Nanomedicine: Nanotechnology, Biology and Medicine
SN - 1549-9634
IS - 5
ER -