Projects per year
Abstract
Semiconductor quantum dots (QDs) have significant advantages over more traditional fluorophores used in fluorescence microscopy including reduced photobleaching, long-term photostability and high quantum yields, but due to limitations in light sources and optics, are often excited far from their optimum excitation wavelengths in the deep-UV. Here, we present a quantitative comparison of the excitation of semiconductor QDs at a wavelength of 280 nm, compared to the longer wavelength of 365 nm, within a cellular environment. We report increased fluorescence intensity and enhanced image quality when using 280 nm excitation compared to 365 nm excitation for cell imaging across multiple datasets, with a highest average fluorescence intensity increase of 3.59-fold. We also find no significant photobleaching of QDs associated with 280 nm excitation and find that on average, ~80% of cells can tolerate exposure to high-intensity 280 nm irradiation over a 6-hour period.
Original language | English |
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Article number | 025004 |
Number of pages | 10 |
Journal | Methods and Applications in Fluorescence |
Volume | 10 |
Issue number | 2 |
Early online date | 9 Mar 2022 |
DOIs | |
Publication status | Published - 1 Apr 2022 |
Keywords
- quantum dots
- fluorescence microscopy
- cellular imaging
- ultraviolet excitation
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Dive into the research topics of 'Enhanced fluorescence from semiconductor quantum dot-labelled cells excited at 280 nm'. Together they form a unique fingerprint.Projects
- 3 Finished
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FASPRI:a new method for increased spatial resolution in surface plasmon imaging of unlabelled living cells.
McConnell, G. (Principal Investigator)
BBSRC (Biotech & Biological Sciences Research Council)
1/01/21 → 31/03/22
Project: Research
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Applications of high-brightness 280nm light emitting diodes in biomedical optical imaging
McConnell, G. (Principal Investigator)
Medical Research Scotland, CoolLED Limited
1/10/18 → 30/09/22
Project: Research - Studentship
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PhD studentship: Application of CoolLED light emitting diodes for new types of super-resolution
McConnell, G. (Principal Investigator)
1/10/15 → 31/03/19
Project: Research - Studentship
Datasets
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Data For: "Enhanced fluorescence from semiconductor quantum dot-labelled cells excited at 280 nm"
Hall, N. (Creator), McFarlane, M. (Creator) & McConnell, G. (Supervisor), University of Strathclyde, 28 Feb 2022
DOI: 10.15129/5b165a66-62b5-48d7-aa08-03ced90df64e, https://github.com/NickHallPhysics/Quantum_dot_image_analysis
Dataset