Enhanced decontamination of C. difficile spores on surfaces via the synergistic action of 405nm light and disinfectants

Sian Moorhead, Michelle MacLean, John E Coia, Scott J MacGregor, John G Anderson

Research output: Contribution to conferencePoster

35 Downloads (Pure)

Abstract

The ability of C. difficile to form spores which can survive for prolonged periods causes significant environmental contamination problems. 405nm light has wide antimicrobial activity against vegetative bacteria, and is being developed for environmental decontamination within hospitals. As expected, spores are more resilient to inactivation. This study aims to establish whether spore susceptibility can be enhanced by combining 405nm light with low concentration chlorinated disinfectants: sodium hypochlorite, Actichlor and Tristel. Spore suspensions were seeded onto surfaces including PVC, stainless steel and vinyl flooring. Disinfectant was added to the surface, and the samples were then exposed to 405nm light at irradiances of ~0.2-225 mWcm-2. Control samples were exposed to 405nm light alone, and disinfectants alone, to establish the sporicidal activity of each agent, and to demonstrate the synergistic effect when combined.
Results demonstrated increased sporicidal activity of 405nm light and low-concentration sodium hypochlorite and Actichlor against C. difficile seeded on vinyl flooring and PVC surfaces, with approximately 3-log10 reductions achieved with up to 66% lower doses than achieved with light alone. Tristel demonstrated limited synergy on vinyl and PVC, whilst all three disinfectants demonstrated minimal synergy on stainless steel. Results are also reported for lower intensity light, as used in the clinical environment. In conclusion, the sporicidal efficacy of 405nm light is enhanced when used alongside chlorinated disinfectants. Further research could potentially lead to the use of lower strength chlorinated disinfectants in combination with 405nm light to provide enhanced decontamination of C. difficile spores in the clinical environment.


Original languageEnglish
Number of pages1
Publication statusPublished - 24 Mar 2016
EventThe Microbiology Society Annual Conference - Arena and Convention Centre (ACC), Liverpool, United Kingdom
Duration: 21 Mar 201624 Mar 2016
file:///D:/USERS/seb13153/Favorites/Downloads/poster_abstract_book_annual_conference_2016.pdf

Conference

ConferenceThe Microbiology Society Annual Conference
CountryUnited Kingdom
CityLiverpool
Period21/03/1624/03/16
Internet address

Fingerprint

Disinfectants
Decontamination
Spores
Light
Polyvinyl Chloride
Polyvinyl chlorides
Sodium Hypochlorite
Stainless Steel
Stainless steel
Sodium
Dosimetry
Suspensions
Bacteria
Contamination

Keywords

  • C. difficile
  • decontamination
  • disinfectants

Cite this

Moorhead, S., MacLean, M., Coia, J. E., MacGregor, S. J., & Anderson, J. G. (2016). Enhanced decontamination of C. difficile spores on surfaces via the synergistic action of 405nm light and disinfectants. Poster session presented at The Microbiology Society Annual Conference, Liverpool, United Kingdom.
Moorhead, Sian ; MacLean, Michelle ; Coia, John E ; MacGregor, Scott J ; Anderson, John G. / Enhanced decontamination of C. difficile spores on surfaces via the synergistic action of 405nm light and disinfectants. Poster session presented at The Microbiology Society Annual Conference, Liverpool, United Kingdom.1 p.
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Moorhead, S, MacLean, M, Coia, JE, MacGregor, SJ & Anderson, JG 2016, 'Enhanced decontamination of C. difficile spores on surfaces via the synergistic action of 405nm light and disinfectants' The Microbiology Society Annual Conference, Liverpool, United Kingdom, 21/03/16 - 24/03/16, .

Enhanced decontamination of C. difficile spores on surfaces via the synergistic action of 405nm light and disinfectants. / Moorhead, Sian; MacLean, Michelle; Coia, John E; MacGregor, Scott J; Anderson, John G.

2016. Poster session presented at The Microbiology Society Annual Conference, Liverpool, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Enhanced decontamination of C. difficile spores on surfaces via the synergistic action of 405nm light and disinfectants

AU - Moorhead, Sian

AU - MacLean, Michelle

AU - Coia, John E

AU - MacGregor, Scott J

AU - Anderson, John G

PY - 2016/3/24

Y1 - 2016/3/24

N2 - The ability of C. difficile to form spores which can survive for prolonged periods causes significant environmental contamination problems. 405nm light has wide antimicrobial activity against vegetative bacteria, and is being developed for environmental decontamination within hospitals. As expected, spores are more resilient to inactivation. This study aims to establish whether spore susceptibility can be enhanced by combining 405nm light with low concentration chlorinated disinfectants: sodium hypochlorite, Actichlor and Tristel. Spore suspensions were seeded onto surfaces including PVC, stainless steel and vinyl flooring. Disinfectant was added to the surface, and the samples were then exposed to 405nm light at irradiances of ~0.2-225 mWcm-2. Control samples were exposed to 405nm light alone, and disinfectants alone, to establish the sporicidal activity of each agent, and to demonstrate the synergistic effect when combined. Results demonstrated increased sporicidal activity of 405nm light and low-concentration sodium hypochlorite and Actichlor against C. difficile seeded on vinyl flooring and PVC surfaces, with approximately 3-log10 reductions achieved with up to 66% lower doses than achieved with light alone. Tristel demonstrated limited synergy on vinyl and PVC, whilst all three disinfectants demonstrated minimal synergy on stainless steel. Results are also reported for lower intensity light, as used in the clinical environment. In conclusion, the sporicidal efficacy of 405nm light is enhanced when used alongside chlorinated disinfectants. Further research could potentially lead to the use of lower strength chlorinated disinfectants in combination with 405nm light to provide enhanced decontamination of C. difficile spores in the clinical environment.

AB - The ability of C. difficile to form spores which can survive for prolonged periods causes significant environmental contamination problems. 405nm light has wide antimicrobial activity against vegetative bacteria, and is being developed for environmental decontamination within hospitals. As expected, spores are more resilient to inactivation. This study aims to establish whether spore susceptibility can be enhanced by combining 405nm light with low concentration chlorinated disinfectants: sodium hypochlorite, Actichlor and Tristel. Spore suspensions were seeded onto surfaces including PVC, stainless steel and vinyl flooring. Disinfectant was added to the surface, and the samples were then exposed to 405nm light at irradiances of ~0.2-225 mWcm-2. Control samples were exposed to 405nm light alone, and disinfectants alone, to establish the sporicidal activity of each agent, and to demonstrate the synergistic effect when combined. Results demonstrated increased sporicidal activity of 405nm light and low-concentration sodium hypochlorite and Actichlor against C. difficile seeded on vinyl flooring and PVC surfaces, with approximately 3-log10 reductions achieved with up to 66% lower doses than achieved with light alone. Tristel demonstrated limited synergy on vinyl and PVC, whilst all three disinfectants demonstrated minimal synergy on stainless steel. Results are also reported for lower intensity light, as used in the clinical environment. In conclusion, the sporicidal efficacy of 405nm light is enhanced when used alongside chlorinated disinfectants. Further research could potentially lead to the use of lower strength chlorinated disinfectants in combination with 405nm light to provide enhanced decontamination of C. difficile spores in the clinical environment.

KW - C. difficile

KW - decontamination

KW - disinfectants

M3 - Poster

ER -

Moorhead S, MacLean M, Coia JE, MacGregor SJ, Anderson JG. Enhanced decontamination of C. difficile spores on surfaces via the synergistic action of 405nm light and disinfectants. 2016. Poster session presented at The Microbiology Society Annual Conference, Liverpool, United Kingdom.