Enhanced bioaffinity sensing using surface plasmons, surface enzyme reactions, nanoparticles and diffraction gratings

H.J. Lee, A.W. Wark, R.M. Corn

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

This paper introduces a novel approach to surface bioaffinity sensing based on the adsorption of nanoparticles onto a gold diffraction grating that supports the excitation of planar surface plasmons. A surface enzymatic amplification reaction is also incorporated into the detection scheme to enhance the sensitivity and utility of the nanoparticle-enhanced diffraction grating (NEDG) sensors. As a demonstration, the detection of microRNA is described where a combination of a surface polymerase reaction and DNA-modified nanoparticles is used to detect the bioaffinity adsorption of the target onto the probe-functionalized gold grating surface. The enzymatically-amplified NEDG sensors possess a great potential for a wide range of applications including the detection of biosecurity agents, DNA and RNA viruses, biomarkers, and proteins.
Original languageEnglish
Pages (from-to)596-601
Number of pages5
JournalAnalyst
Volume133
Issue number5
DOIs
Publication statusPublished - 2008

Keywords

  • bioaffinity sensing
  • surface plasmons
  • surface enzyme reactions
  • nanoparticles
  • diffraction gratings

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