Abstract
We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.
| Language | English |
|---|---|
| Title of host publication | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII |
| Editors | Alexander N. Cartwright, Dan V. Nicolau |
| Place of Publication | Bellingham WA |
| Number of pages | 6 |
| Volume | 9721 |
| DOIs | |
| Publication status | Published - 2016 |
| Event | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States Duration: 15 Feb 2016 → 17 Feb 2016 |
Publication series
| Name | Proceedings of Spie |
|---|---|
| Publisher | SPIE |
| Volume | 9721 |
| ISSN (Print) | 1605-7422 |
| ISSN (Electronic) | 2410-9045 |
Conference
| Conference | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 15/02/16 → 17/02/16 |
Fingerprint
Keywords
- biosensors
- colorimetry
- dip-pen nanolithography
- DNA assembly
- electron-beam lithography
- naked-eye detection
- plasmonics
- surface enhanced Raman spectroscopy
- lab-on-a-chip
- scanning probe lithography
- molecular self-assembly
- multiplexing
Cite this
}
Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering. / Heydari, Esmaeil; Mabbott, Samuel; Thompson, David; Graham, Duncan; Cooper, Jonathan M.; Clark, Alasdair W.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. ed. / Alexander N. Cartwright; Dan V. Nicolau. Vol. 9721 Bellingham WA, 2016. 972105 (Proceedings of Spie; Vol. 9721).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book
TY - GEN
T1 - Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering
AU - Heydari, Esmaeil
AU - Mabbott, Samuel
AU - Thompson, David
AU - Graham, Duncan
AU - Cooper, Jonathan M.
AU - Clark, Alasdair W.
N1 - Heydari, Esmaeil; Mabbott, Samuel; Thompson, David; Graham, Duncan; Cooper, Jonathan M.; Clark, Alasdair W. "Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering." Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. ed. / Alexander N. Cartwright; Dan V. Nicolau. Proceedings of Spie Vol. 9721 Bellingham WA : SPIE, 2016. 972105, 2016. Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2209108
PY - 2016
Y1 - 2016
N2 - We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.
AB - We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.
KW - biosensors
KW - colorimetry
KW - dip-pen nanolithography
KW - DNA assembly
KW - electron-beam lithography
KW - naked-eye detection
KW - plasmonics
KW - surface enhanced Raman spectroscopy
KW - lab-on-a-chip
KW - scanning probe lithography
KW - molecular self-assembly
KW - multiplexing
UR - http://www.scopus.com/inward/record.url?scp=84982187258&partnerID=8YFLogxK
UR - http://proceedings.spiedigitallibrary.org/volume.aspx?conferenceid=3624&volumeid=17545
UR - http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016
U2 - 10.1117/12.2209108
DO - 10.1117/12.2209108
M3 - Conference contribution book
SN - 9781628419559
VL - 9721
T3 - Proceedings of Spie
BT - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
A2 - Cartwright, Alexander N.
A2 - Nicolau, Dan V.
CY - Bellingham WA
ER -