Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering

Esmaeil Heydari, Samuel Mabbott, David Thompson, Duncan Graham, Jonathan M. Cooper, Alasdair W. Clark

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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Abstract

We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
EditorsAlexander N. Cartwright, Dan V. Nicolau
Place of PublicationBellingham WA
Number of pages6
Volume9721
DOIs
Publication statusPublished - 2016
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States
Duration: 15 Feb 201617 Feb 2016

Publication series

NameProceedings of Spie
PublisherSPIE
Volume9721
ISSN (Print)1605-7422
ISSN (Electronic)2410-9045

Conference

ConferenceNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
CountryUnited States
CitySan Francisco
Period15/02/1617/02/16

Keywords

  • biosensors
  • colorimetry
  • dip-pen nanolithography
  • DNA assembly
  • electron-beam lithography
  • naked-eye detection
  • plasmonics
  • surface enhanced Raman spectroscopy
  • lab-on-a-chip
  • scanning probe lithography
  • molecular self-assembly
  • multiplexing

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  • Cite this

    Heydari, E., Mabbott, S., Thompson, D., Graham, D., Cooper, J. M., & Clark, A. W. (2016). Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering. In A. N. Cartwright, & D. V. Nicolau (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII (Vol. 9721). [972105] (Proceedings of Spie; Vol. 9721). Bellingham WA. https://doi.org/10.1117/12.2209108