Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering

Esmaeil Heydari, Samuel Mabbott, David Thompson, Duncan Graham, Jonathan M. Cooper, Alasdair W. Clark

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.

LanguageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
EditorsAlexander N. Cartwright, Dan V. Nicolau
Place of PublicationBellingham WA
Number of pages6
Volume9721
DOIs
Publication statusPublished - 2016
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States
Duration: 15 Feb 201617 Feb 2016

Publication series

NameProceedings of Spie
PublisherSPIE
Volume9721
ISSN (Print)1605-7422
ISSN (Electronic)2410-9045

Conference

ConferenceNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
CountryUnited States
CitySan Francisco
Period15/02/1617/02/16

Fingerprint

colorimetry
Colorimetry
Raman Spectrum Analysis
Genetic Markers
Raman scattering
DNA
deoxyribonucleic acid
Color
Point-of-Care Systems
engineering
Metal Nanoparticles
Raman spectra
Nanostructures
Biosensing Techniques
scattering
markers
Nanolithography
Nanophotonics
Lab-on-a-chip
DNA sequences

Keywords

  • biosensors
  • colorimetry
  • dip-pen nanolithography
  • DNA assembly
  • electron-beam lithography
  • naked-eye detection
  • plasmonics
  • surface enhanced Raman spectroscopy
  • lab-on-a-chip
  • scanning probe lithography
  • molecular self-assembly
  • multiplexing

Cite this

Heydari, E., Mabbott, S., Thompson, D., Graham, D., Cooper, J. M., & Clark, A. W. (2016). Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering. In A. N. Cartwright, & D. V. Nicolau (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII (Vol. 9721). [972105] (Proceedings of Spie; Vol. 9721). Bellingham WA. https://doi.org/10.1117/12.2209108
Heydari, Esmaeil ; Mabbott, Samuel ; Thompson, David ; Graham, Duncan ; Cooper, Jonathan M. ; Clark, Alasdair W. / Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering. Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. editor / Alexander N. Cartwright ; Dan V. Nicolau. Vol. 9721 Bellingham WA, 2016. (Proceedings of Spie).
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abstract = "We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.",
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Heydari, E, Mabbott, S, Thompson, D, Graham, D, Cooper, JM & Clark, AW 2016, Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering. in AN Cartwright & DV Nicolau (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. vol. 9721, 972105, Proceedings of Spie, vol. 9721, Bellingham WA, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, San Francisco, United States, 15/02/16. https://doi.org/10.1117/12.2209108

Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering. / Heydari, Esmaeil; Mabbott, Samuel; Thompson, David; Graham, Duncan; Cooper, Jonathan M.; Clark, Alasdair W.

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. ed. / Alexander N. Cartwright; Dan V. Nicolau. Vol. 9721 Bellingham WA, 2016. 972105 (Proceedings of Spie; Vol. 9721).

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

TY - GEN

T1 - Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering

AU - Heydari, Esmaeil

AU - Mabbott, Samuel

AU - Thompson, David

AU - Graham, Duncan

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N1 - Heydari, Esmaeil; Mabbott, Samuel; Thompson, David; Graham, Duncan; Cooper, Jonathan M.; Clark, Alasdair W. "Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering." Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. ed. / Alexander N. Cartwright; Dan V. Nicolau. Proceedings of Spie Vol. 9721 Bellingham WA : SPIE, 2016. 972105, 2016. Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2209108

PY - 2016

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N2 - We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.

AB - We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.

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KW - dip-pen nanolithography

KW - DNA assembly

KW - electron-beam lithography

KW - naked-eye detection

KW - plasmonics

KW - surface enhanced Raman spectroscopy

KW - lab-on-a-chip

KW - scanning probe lithography

KW - molecular self-assembly

KW - multiplexing

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SN - 9781628419559

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CY - Bellingham WA

ER -

Heydari E, Mabbott S, Thompson D, Graham D, Cooper JM, Clark AW. Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering. In Cartwright AN, Nicolau DV, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. Vol. 9721. Bellingham WA. 2016. 972105. (Proceedings of Spie). https://doi.org/10.1117/12.2209108