Energy transfer between a biological labelling dye and gold nanorods

Chris Racknor, Mahi R Singh, Yinan Zhang, David J S Birch, Yu Chen

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We have demonstrated energy transfer between a biological labelling dye (Alexa Fluor 405) and gold nanorods experimentally and theoretically. The fluorescence lifetime imaging microscopy and density matrix method are used to study a hybrid system of dye and nanorods under one- and two-photon excitations. Energy transfer between dye and nanorods via the dipole–dipole interaction is found to cause a decrease in the fluorescence lifetime change. Enhanced energy transfer from dye to nanorods is measured in the presence of an increased density of nanorods. This study has potential applications in fluorescence lifetime-based intra-cellular sensing of bio-analytes as well as nuclear targeting cancer therapy
Original languageEnglish
Article number015002
Number of pages6
JournalMethods and Applications in Fluorescence
Issue number1
Early online date15 Nov 2013
Publication statusPublished - 1 Mar 2014


  • thin films
  • energy transfer
  • gold nanorods
  • biological labelling dye
  • biological physics


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