Electrochemiluminescent monolayers on metal oxide electrodes: detection of amino acids

Lynn Dennany, Emmet J. O'Reilly, Tia E. Keyes, Robert J. Forster

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The electrochemical and photochemical properties of [Ru(bPY)(2)dcb]Cl-2 monolayers and their subsequent use for the detection of amino acids, e.g., proline, is reported; dcb is 4,4'-dicarboxy-2,2'-bipyridine and bpy is 2,2'-bipyridine. The ability of such a complex to form stable monolayers enables its utilization for the detection of oxalate and several amino acids, producing significant ECL. The electrochemical response of the Ru2+/3+ couple is electrochemically reversible and the monolayers show excellent stability with the response decreasing by less than 8% after continuous scanning over 4 h. The ECL and current responses increase linearly with increasing analyte concentration 0.2 <=, [Proline] <= 1 nM while for hydroxy-proline the dynamic range is from 1 to 10 nM. The ECL signal was monitored at similar to 644 nm, representing the lambda(max) for the [Ru(bPY)2dcb]Cl-2 monolayers. This wavelength is shifted by approximately 28 nm compared to the photoexcited lambda(max) for the same system. This technique is simple, rapid, selective and sensitive, and shows potential for the high-throughput quantitation of amino acids if adapted with other techniques such as FIA or LC systems. (c) 2006 Elsevier B.V. All rights reserved.

LanguageEnglish
Pages1588-1594
Number of pages7
JournalElectrochemistry Communications
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Emitter coupled logic circuits
Oxides
Amino acids
Monolayers
Metals
Amino Acids
2,2'-Dipyridyl
Electrodes
Proline
Hydroxyproline
Oxalates
Throughput
Scanning
Wavelength

Keywords

  • electrochemiluminescence
  • monolayer
  • monolayer emission
  • amino acid
  • electroanalysis
  • luminescence
  • sensitized solar-cells
  • electrogenerated chemiluminescence
  • capillary-electrophoresis
  • electrochemistry
  • complexes
  • emission
  • nafion
  • semiconductor

Cite this

Dennany, Lynn ; O'Reilly, Emmet J. ; Keyes, Tia E. ; Forster, Robert J. / Electrochemiluminescent monolayers on metal oxide electrodes: detection of amino acids. In: Electrochemistry Communications. 2006 ; Vol. 8, No. 10. pp. 1588-1594.
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Electrochemiluminescent monolayers on metal oxide electrodes: detection of amino acids. / Dennany, Lynn; O'Reilly, Emmet J.; Keyes, Tia E.; Forster, Robert J.

In: Electrochemistry Communications, Vol. 8, No. 10, 10.2006, p. 1588-1594.

Research output: Contribution to journalArticle

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AU - Dennany, Lynn

AU - O'Reilly, Emmet J.

AU - Keyes, Tia E.

AU - Forster, Robert J.

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N2 - The electrochemical and photochemical properties of [Ru(bPY)(2)dcb]Cl-2 monolayers and their subsequent use for the detection of amino acids, e.g., proline, is reported; dcb is 4,4'-dicarboxy-2,2'-bipyridine and bpy is 2,2'-bipyridine. The ability of such a complex to form stable monolayers enables its utilization for the detection of oxalate and several amino acids, producing significant ECL. The electrochemical response of the Ru2+/3+ couple is electrochemically reversible and the monolayers show excellent stability with the response decreasing by less than 8% after continuous scanning over 4 h. The ECL and current responses increase linearly with increasing analyte concentration 0.2 <=, [Proline] <= 1 nM while for hydroxy-proline the dynamic range is from 1 to 10 nM. The ECL signal was monitored at similar to 644 nm, representing the lambda(max) for the [Ru(bPY)2dcb]Cl-2 monolayers. This wavelength is shifted by approximately 28 nm compared to the photoexcited lambda(max) for the same system. This technique is simple, rapid, selective and sensitive, and shows potential for the high-throughput quantitation of amino acids if adapted with other techniques such as FIA or LC systems. (c) 2006 Elsevier B.V. All rights reserved.

AB - The electrochemical and photochemical properties of [Ru(bPY)(2)dcb]Cl-2 monolayers and their subsequent use for the detection of amino acids, e.g., proline, is reported; dcb is 4,4'-dicarboxy-2,2'-bipyridine and bpy is 2,2'-bipyridine. The ability of such a complex to form stable monolayers enables its utilization for the detection of oxalate and several amino acids, producing significant ECL. The electrochemical response of the Ru2+/3+ couple is electrochemically reversible and the monolayers show excellent stability with the response decreasing by less than 8% after continuous scanning over 4 h. The ECL and current responses increase linearly with increasing analyte concentration 0.2 <=, [Proline] <= 1 nM while for hydroxy-proline the dynamic range is from 1 to 10 nM. The ECL signal was monitored at similar to 644 nm, representing the lambda(max) for the [Ru(bPY)2dcb]Cl-2 monolayers. This wavelength is shifted by approximately 28 nm compared to the photoexcited lambda(max) for the same system. This technique is simple, rapid, selective and sensitive, and shows potential for the high-throughput quantitation of amino acids if adapted with other techniques such as FIA or LC systems. (c) 2006 Elsevier B.V. All rights reserved.

KW - electrochemiluminescence

KW - monolayer

KW - monolayer emission

KW - amino acid

KW - electroanalysis

KW - luminescence

KW - sensitized solar-cells

KW - electrogenerated chemiluminescence

KW - capillary-electrophoresis

KW - electrochemistry

KW - complexes

KW - emission

KW - nafion

KW - semiconductor

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