This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.
- recombinant antibodies
- cardiac biomarkers
- c-reactive proteins
O'Reilly, E. J., Conroy, P. J., Hearty, S., Keyes, T. E., O'Kennedy, R., Forster, R. J., & Dennany, L. (2015). Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection. RSC Advances, 5(83), 67874-67877. https://doi.org/10.1039/C5RA08450D