Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly; Paul J. Conroy; Stephen Hearty; Tia E. Keyes; Richard O'Kennedy; Robert J. Forster; Lynn Dennany

doi:10.1039/C5RA08450D

Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly, Paul J. Conroy, Stephen Hearty, Tia E. Keyes, Richard O'Kennedy, Robert J. Forster, Lynn Dennany

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

86 Downloads (Pure)

Abstract

This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL^-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

Original language	English
Pages (from-to)	67874-67877
Number of pages	4
Journal	RSC Advances
Volume	5
Issue number	83
DOIs	https://doi.org/10.1039/C5RA08450D
Publication status	Published - 31 Jul 2015

Keywords

recombinant antibodies
cardiac biomarkers
c-reactive proteins

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10.1039/C5RA08450D

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Lynn Dennany
- lynn.dennany@strath.ac.uk
- Pure And Applied Chemistry - Senior Lecturer
- Health and Wellbeing
- Measurement Science and Enabling Technologies
Person: Academic

1 Participation in conference

International Meeting of Electrogenerated Chemiluminescence
Lynn Dennany (Speaker)
7 Sep 2014 → 10 Sep 2014
Activity: Participating in or organising an event types › Participation in conference

Cite this

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title = "Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection",

abstract = "This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.",

keywords = "recombinant antibodies, cardiac biomarkers, c-reactive proteins",

author = "O'Reilly, {Emmet J.} and Conroy, {Paul J.} and Stephen Hearty and Keyes, {Tia E.} and Richard O'Kennedy and Forster, {Robert J.} and Lynn Dennany",

year = "2015",

month = jul,

day = "31",

doi = "10.1039/C5RA08450D",

language = "English",

volume = "5",

pages = "67874--67877",

journal = "RSC Advances",

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publisher = "Royal Society of Chemistry",

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Electrochemiluminescence platform for the detection of C-reactive proteins : application of recombinant antibody technology to cardiac biomarker detection. / O'Reilly, Emmet J.; Conroy, Paul J.; Hearty, Stephen; Keyes, Tia E.; O'Kennedy, Richard; Forster, Robert J.; Dennany, Lynn.

In: RSC Advances, Vol. 5, No. 83, 31.07.2015, p. 67874-67877.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Electrochemiluminescence platform for the detection of C-reactive proteins

T2 - application of recombinant antibody technology to cardiac biomarker detection

AU - O'Reilly, Emmet J.

AU - Conroy, Paul J.

AU - Hearty, Stephen

AU - Keyes, Tia E.

AU - O'Kennedy, Richard

AU - Forster, Robert J.

AU - Dennany, Lynn

PY - 2015/7/31

Y1 - 2015/7/31

N2 - This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

AB - This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

KW - recombinant antibodies

KW - cardiac biomarkers

KW - c-reactive proteins

U2 - 10.1039/C5RA08450D

DO - 10.1039/C5RA08450D

M3 - Article

VL - 5

SP - 67874

EP - 67877

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 83

ER -

Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Abstract

Keywords

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Lynn Dennany

International Meeting of Electrogenerated Chemiluminescence

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