Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly, Paul J. Conroy, Stephen Hearty, Tia E. Keyes, Richard O'Kennedy, Robert J. Forster, Lynn Dennany

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Abstract

This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.
Original languageEnglish
Pages (from-to)67874-67877
Number of pages4
JournalRSC Advances
Volume5
Issue number83
DOIs
Publication statusPublished - 31 Jul 2015

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Keywords

  • recombinant antibodies
  • cardiac biomarkers
  • c-reactive proteins

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