Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly; Paul J. Conroy; Stephen Hearty; Tia E. Keyes; Richard O'Kennedy; Robert J. Forster; Lynn Dennany

doi:10.1039/C5RA08450D

Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly, Paul J. Conroy, Stephen Hearty, Tia E. Keyes, Richard O'Kennedy, Robert J. Forster, Lynn Dennany

Pure And Applied Chemistry

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17 Citations (Scopus)

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Abstract

This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL^-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

Original language	English
Pages (from-to)	67874-67877
Number of pages	4
Journal	RSC Advances
Volume	5
Issue number	83
DOIs	https://doi.org/10.1039/C5RA08450D
Publication status	Published - 31 Jul 2015

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Keywords

recombinant antibodies
cardiac biomarkers
c-reactive proteins

Cite this

O'Reilly, E. J., Conroy, P. J., Hearty, S., Keyes, T. E., O'Kennedy, R., Forster, R. J., & Dennany, L. (2015). Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection. RSC Advances, 5(83), 67874-67877. https://doi.org/10.1039/C5RA08450D

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title = "Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection",

abstract = "This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.",

keywords = "recombinant antibodies, cardiac biomarkers, c-reactive proteins",

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language = "English",

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journal = "RSC Advances",

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publisher = "Royal Society of Chemistry",

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Electrochemiluminescence platform for the detection of C-reactive proteins : application of recombinant antibody technology to cardiac biomarker detection. / O'Reilly, Emmet J.; Conroy, Paul J.; Hearty, Stephen; Keyes, Tia E.; O'Kennedy, Richard; Forster, Robert J.; Dennany, Lynn.

In: RSC Advances, Vol. 5, No. 83, 31.07.2015, p. 67874-67877.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Electrochemiluminescence platform for the detection of C-reactive proteins

T2 - application of recombinant antibody technology to cardiac biomarker detection

AU - O'Reilly, Emmet J.

AU - Conroy, Paul J.

AU - Hearty, Stephen

AU - Keyes, Tia E.

AU - O'Kennedy, Richard

AU - Forster, Robert J.

AU - Dennany, Lynn

PY - 2015/7/31

Y1 - 2015/7/31

N2 - This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

AB - This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

KW - recombinant antibodies

KW - cardiac biomarkers

KW - c-reactive proteins

U2 - 10.1039/C5RA08450D

DO - 10.1039/C5RA08450D

M3 - Article

VL - 5

SP - 67874

EP - 67877

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 83

ER -

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10.1039/C5RA08450D

O'Reilly-etal-RSCA2015-Electrochemiluminescence-platform-for-the-detection-of-C-reactive-proteinsAccepted author manuscript, 646 KB

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International Meeting of Electrogenerated Chemiluminescence

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Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

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International Meeting of Electrogenerated Chemiluminescence