Electrochemiluminescence nanoimmunosensor for CD63 protein using a carbon nanochips/iron oxide/nafion-nanocomposite modified mesoporous carbon interface

Juthi Adhikari, Mohammad Rizwan, Lynn Dennany, Minhaz Uddin Ahmed

Research output: Contribution to journalArticle

Abstract

The detection of extracellular vesicles, or exosomes are important mediators in intercellular communication and often play a role in cancer progression. CD63 is a key exosomal protein due to its distinctive cellular functions and association with many cancers. This describes a label-free electrochemiluminescence (ECL) nanoimmunosensor for the detection of CD63 protein over mesoporous carbon screen-printed electrode (MC-SPE) modified with novel nanocomposite of carbon nanochips (CNCs), iron oxide (Fe3O4) and nafion (NAF) . Fourier-transform infrared spectroscopy and field emission scanning electron microscopy were used to analyse nanocomposite. All the analytical performance of fabricated CD63 immunosensor were conducted applying ECL. In spite of the simple fabrication strategies utilized, the fabricated immunosensor showcased a broad linear range to detect CD63 from 100 fg mL-1 to 10 ng mL-1, with a limit of detection of 100 fg mL-1, excellent selectivity, interference-resistance capability and potential to detect CD63 in real clinical samples.
Original languageEnglish
Article number108755
JournalMeasurement
Volume170
Early online date23 Nov 2020
DOIs
Publication statusE-pub ahead of print - 23 Nov 2020

Keywords

  • CD63 protein
  • electrochemiluminescence
  • mesoporous carbon
  • carbon nanochips
  • immunosensor

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