Abstract
Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasmamembrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasmamembrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localization of phosphatidylinositol 4-kinase type IIIα to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIα impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane.
Original language | English |
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Article number | BSR20221181 |
Pages (from-to) | 1-13 |
Number of pages | 13 |
Journal | Bioscience Reports |
Volume | 42 |
Issue number | 7 |
Early online date | 23 Jun 2022 |
DOIs | |
Publication status | Published - 8 Jul 2022 |
Keywords
- adipocytes
- glucose transport
- GLUT4
- insulin
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Dive into the research topics of 'EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes'. Together they form a unique fingerprint.Datasets
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dSTORM imaging data files for 'EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes'
Gould, G. (Creator), Geiser, A. (Data Collector), Koester, A. M. (Data Collector) & Morris, S. (Data Collector), University of Strathclyde, 3 Mar 2023
DOI: 10.15129/e0c00ca5-4f79-4797-87d3-c590595c8da1
Dataset
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Analysis of GLUT4 dispersal in cultured adipocytes
Gould, G. (Creator), Koester, A. M. (Data Collector), Morris, S. (Data Collector) & Geiser, A. (Data Collector), University of Strathclyde, 3 Mar 2023
DOI: 10.15129/3c4691b7-2f26-42fb-8886-20a7dbbe1d3f
Dataset