EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes

Anna M.  Koester; Angéline Geiser; Kamilla M.E. Laidlaw; Silke Morris; Marie F.A. Cutiongco; Laura Stirrat; Nikolaj Gadegaard; Eckhard Boles; Hannah L. Black; Nia J. Bryant; Gwyn W. Gould

doi:10.1042/BSR20221181

EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes

Anna M. Koester, Angéline Geiser, Kamilla M.E. Laidlaw, Silke Morris, Marie F.A. Cutiongco, Laura Stirrat, Nikolaj Gadegaard, Eckhard Boles , Hannah L. Black, Nia J. Bryant, Gwyn W. Gould

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

50 Downloads (Pure)

Abstract

Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasmamembrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasmamembrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localization of phosphatidylinositol 4-kinase type IIIα to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIα impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane.

Original language	English
Article number	BSR20221181
Pages (from-to)	1-13
Number of pages	13
Journal	Bioscience Reports
Volume	42
Issue number	7
Early online date	23 Jun 2022
DOIs	https://doi.org/10.1042/BSR20221181
Publication status	Published - 8 Jul 2022

Keywords

adipocytes
glucose transport
GLUT4
insulin

Access to Document

10.1042/BSR20221181Licence: CC BY 4.0

Koester-etal-BR-2022-EFR3-and-phosphatidylinositol-4-kinase-III[alpha]-regulate-insulin-stimulated-glucose-transportFinal published version, 2.33 MBLicence: CC BY 4.0

Doctoral Training Partnership 2020-2021 University of Strathclyde | Geiser, Angéline
Gould, G. (Principal Investigator), Currie, S. (Co-investigator) & Geiser, A. (Research Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/10/20 → 14/11/24
Project: Research Studentship - Internally Allocated

Analysis of GLUT4 dispersal in cultured adipocytes
Gould, G. (Creator), Koester, A. M. (Data Collector), Morris, S. (Data Collector) & Geiser, A. (Data Collector), University of Strathclyde, 3 Mar 2023
DOI: 10.15129/3c4691b7-2f26-42fb-8886-20a7dbbe1d3f
Dataset
dSTORM imaging data files for 'EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes'
Gould, G. (Creator), Geiser, A. (Data Collector), Koester, A. M. (Data Collector) & Morris, S. (Data Collector), University of Strathclyde, 3 Mar 2023
DOI: 10.15129/e0c00ca5-4f79-4797-87d3-c590595c8da1
Dataset

1 Organiser of major conference
1 Invited talk
1 Visiting an external academic institution

28th European Association for the Study of Diabetes WorkshopFrom Pixels to Pancreas and Patients: Imaging in Diabetes Research
Gould, G. (Organiser)
22 Aug 2025 → 25 Aug 2025
Activity: Participating in or organising an event types › Organiser of major conference
5th Swedish Diabetes Summit
Gould, G. (Speaker)
21 May 2025 → 23 May 2025
Activity: Talk or presentation types › Invited talk
Tohoku University
Gould, G. (Visiting researcher)
7 Oct 2024 → 21 Oct 2024
Activity: Visiting an external institution types › Visiting an external academic institution

Cite this

Koester, A. M., Geiser, A., Laidlaw, K. M. E., Morris, S., Cutiongco, M. F. A., Stirrat, L., Gadegaard, N., Boles , E., Black, H. L., Bryant, N. J., & Gould, G. W. (2022). EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes. Bioscience Reports, 42(7), 1-13. Article BSR20221181. https://doi.org/10.1042/BSR20221181

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title = "EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes",

abstract = "Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasmamembrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasmamembrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localization of phosphatidylinositol 4-kinase type IIIα to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIα impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane.",

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Koester, AM, Geiser, A, Laidlaw, KME, Morris, S, Cutiongco, MFA, Stirrat, L, Gadegaard, N, Boles , E, Black, HL, Bryant, NJ & Gould, GW 2022, 'EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes', Bioscience Reports, vol. 42, no. 7, BSR20221181, pp. 1-13. https://doi.org/10.1042/BSR20221181

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T1 - EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes

AU - Koester, Anna M.

AU - Geiser, Angéline

AU - Laidlaw, Kamilla M.E.

AU - Morris, Silke

AU - Cutiongco, Marie F.A.

AU - Stirrat, Laura

AU - Gadegaard, Nikolaj

AU - Boles , Eckhard

AU - Black, Hannah L.

AU - Bryant, Nia J.

AU - Gould, Gwyn W.

PY - 2022/7/8

Y1 - 2022/7/8

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AB - Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasmamembrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasmamembrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localization of phosphatidylinositol 4-kinase type IIIα to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIα impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane.

KW - adipocytes

KW - glucose transport

KW - GLUT4

KW - insulin

U2 - 10.1042/BSR20221181

DO - 10.1042/BSR20221181

M3 - Article

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VL - 42

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JO - Bioscience Reports

JF - Bioscience Reports

IS - 7

M1 - BSR20221181

ER -

EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes

Abstract

Keywords

Access to Document

Fingerprint

Projects

Doctoral Training Partnership 2020-2021 University of Strathclyde | Geiser, Angéline

Datasets

Analysis of GLUT4 dispersal in cultured adipocytes

dSTORM imaging data files for 'EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes'

Activities

28th European Association for the Study of Diabetes WorkshopFrom Pixels to Pancreas and Patients: Imaging in Diabetes Research

5th Swedish Diabetes Summit

Tohoku University

Cite this