Efflux pump gene expression in Erwinia chrysanthemi is induced by exposure to phenolic acids

Ramani S. Ravirala, Ravi D. Barabote, David M. Wheeler, Sylvie Reverchon, Ouwatha Tatum, Jeremy Malouf, Hui Liu, Leighton Pritchard, Peter E. Hedley, Paul R. J. Birch, Ian K. Toth, Paxton Payton, Michael J. D. San Francisco

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Salicylic acid (SA) is an important signaling molecule in local and systemic plant resistance. Following infection by microbial pathogens and the initial oxidative burst in plants, SA accumulation functions in the amplification of defense gene expression. Production of pathogenesis-related proteins and toxic antimicrobial chemicals serves to protect the plant from infection. Successful microbial pathogens utilize a variety of mechanisms to rid themselves of toxic antimicrobial compounds. Important among these mechanisms are multidrug-resistance pumps that bring about the active efflux of toxic compounds from microbial cells. Here, we show that a combination SA and its precursors, t-cinnamic acid and benzoic acid, can activate expression of specific multidrug efflux pump-encoding genes in the plant pathogen Erwinia chrysanthemi and enhance survival of the bacterium in the presence of model as well as plant-derived antimicrobial chemicals. This ability of plant-pathogenic bacteria to co-opt plant defense-signaling molecules to activate multidrug efflux pumps may have evolved to ensure bacterial survival in susceptible host plants.

LanguageEnglish
Pages313-320
Number of pages8
JournalMolecular Plant-Microbe Interactions
Volume20
Issue number3
DOIs
Publication statusPublished - 1 Mar 2007

Fingerprint

Pectobacterium chrysanthemi
Erwinia chrysanthemi
phenolic acids
transporters
Gene Expression
gene expression
salicylic acid
Salicylic Acid
Poisons
anti-infective agents
plant pathogenic bacteria
pathogenesis-related proteins
pathogens
cinnamic acid
multiple drug resistance
benzoic acid
toxic substances
MDR Genes
Bacteria
plant pathogens

Keywords

  • acrA
  • bacterial resistance
  • emrA

Cite this

Ravirala, R. S., Barabote, R. D., Wheeler, D. M., Reverchon, S., Tatum, O., Malouf, J., ... San Francisco, M. J. D. (2007). Efflux pump gene expression in Erwinia chrysanthemi is induced by exposure to phenolic acids. 20(3), 313-320. https://doi.org/10.1094/MPMI-20-3-0313
Ravirala, Ramani S. ; Barabote, Ravi D. ; Wheeler, David M. ; Reverchon, Sylvie ; Tatum, Ouwatha ; Malouf, Jeremy ; Liu, Hui ; Pritchard, Leighton ; Hedley, Peter E. ; Birch, Paul R. J. ; Toth, Ian K. ; Payton, Paxton ; San Francisco, Michael J. D. / Efflux pump gene expression in Erwinia chrysanthemi is induced by exposure to phenolic acids. 2007 ; Vol. 20, No. 3. pp. 313-320.
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abstract = "Salicylic acid (SA) is an important signaling molecule in local and systemic plant resistance. Following infection by microbial pathogens and the initial oxidative burst in plants, SA accumulation functions in the amplification of defense gene expression. Production of pathogenesis-related proteins and toxic antimicrobial chemicals serves to protect the plant from infection. Successful microbial pathogens utilize a variety of mechanisms to rid themselves of toxic antimicrobial compounds. Important among these mechanisms are multidrug-resistance pumps that bring about the active efflux of toxic compounds from microbial cells. Here, we show that a combination SA and its precursors, t-cinnamic acid and benzoic acid, can activate expression of specific multidrug efflux pump-encoding genes in the plant pathogen Erwinia chrysanthemi and enhance survival of the bacterium in the presence of model as well as plant-derived antimicrobial chemicals. This ability of plant-pathogenic bacteria to co-opt plant defense-signaling molecules to activate multidrug efflux pumps may have evolved to ensure bacterial survival in susceptible host plants.",
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Ravirala, RS, Barabote, RD, Wheeler, DM, Reverchon, S, Tatum, O, Malouf, J, Liu, H, Pritchard, L, Hedley, PE, Birch, PRJ, Toth, IK, Payton, P & San Francisco, MJD 2007, 'Efflux pump gene expression in Erwinia chrysanthemi is induced by exposure to phenolic acids' vol. 20, no. 3, pp. 313-320. https://doi.org/10.1094/MPMI-20-3-0313

Efflux pump gene expression in Erwinia chrysanthemi is induced by exposure to phenolic acids. / Ravirala, Ramani S.; Barabote, Ravi D.; Wheeler, David M.; Reverchon, Sylvie; Tatum, Ouwatha; Malouf, Jeremy; Liu, Hui; Pritchard, Leighton; Hedley, Peter E.; Birch, Paul R. J.; Toth, Ian K.; Payton, Paxton; San Francisco, Michael J. D.

Vol. 20, No. 3, 01.03.2007, p. 313-320.

Research output: Contribution to journalArticle

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T1 - Efflux pump gene expression in Erwinia chrysanthemi is induced by exposure to phenolic acids

AU - Ravirala, Ramani S.

AU - Barabote, Ravi D.

AU - Wheeler, David M.

AU - Reverchon, Sylvie

AU - Tatum, Ouwatha

AU - Malouf, Jeremy

AU - Liu, Hui

AU - Pritchard, Leighton

AU - Hedley, Peter E.

AU - Birch, Paul R. J.

AU - Toth, Ian K.

AU - Payton, Paxton

AU - San Francisco, Michael J. D.

PY - 2007/3/1

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N2 - Salicylic acid (SA) is an important signaling molecule in local and systemic plant resistance. Following infection by microbial pathogens and the initial oxidative burst in plants, SA accumulation functions in the amplification of defense gene expression. Production of pathogenesis-related proteins and toxic antimicrobial chemicals serves to protect the plant from infection. Successful microbial pathogens utilize a variety of mechanisms to rid themselves of toxic antimicrobial compounds. Important among these mechanisms are multidrug-resistance pumps that bring about the active efflux of toxic compounds from microbial cells. Here, we show that a combination SA and its precursors, t-cinnamic acid and benzoic acid, can activate expression of specific multidrug efflux pump-encoding genes in the plant pathogen Erwinia chrysanthemi and enhance survival of the bacterium in the presence of model as well as plant-derived antimicrobial chemicals. This ability of plant-pathogenic bacteria to co-opt plant defense-signaling molecules to activate multidrug efflux pumps may have evolved to ensure bacterial survival in susceptible host plants.

AB - Salicylic acid (SA) is an important signaling molecule in local and systemic plant resistance. Following infection by microbial pathogens and the initial oxidative burst in plants, SA accumulation functions in the amplification of defense gene expression. Production of pathogenesis-related proteins and toxic antimicrobial chemicals serves to protect the plant from infection. Successful microbial pathogens utilize a variety of mechanisms to rid themselves of toxic antimicrobial compounds. Important among these mechanisms are multidrug-resistance pumps that bring about the active efflux of toxic compounds from microbial cells. Here, we show that a combination SA and its precursors, t-cinnamic acid and benzoic acid, can activate expression of specific multidrug efflux pump-encoding genes in the plant pathogen Erwinia chrysanthemi and enhance survival of the bacterium in the presence of model as well as plant-derived antimicrobial chemicals. This ability of plant-pathogenic bacteria to co-opt plant defense-signaling molecules to activate multidrug efflux pumps may have evolved to ensure bacterial survival in susceptible host plants.

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M3 - Article

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SP - 313

EP - 320

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ER -

Ravirala RS, Barabote RD, Wheeler DM, Reverchon S, Tatum O, Malouf J et al. Efflux pump gene expression in Erwinia chrysanthemi is induced by exposure to phenolic acids. 2007 Mar 1;20(3):313-320. https://doi.org/10.1094/MPMI-20-3-0313