Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria

Hikmate Abriouel, Leyre Lavilla Lerma, Beatriz Pérez Montoro, Esther Alonso, Charles W. Knapp, Natacha Cabellero Gómez, Antonio Gálvez, Nabil Benomar

Research output: Contribution to journalArticle

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Abstract

We evaluated the efficacy of a new disinfectant product, HLE, to inhibit multiple species of planktonic and biofilm bacterial cultures. The HLE disinfectant comprised of EDTA, lactic acid and hydrogen peroxide, and our data indicated that the disinfectant had effective antimicrobial and anti-biofilm activity even at low concentrations (0.15% to 0.4% HLE, v/v). Furthermore, the HLE disinfectant destabilized biofilm structures eradicated them due to the synergistic effect of EDTA and both antimicrobials (lactic acid and hydrogen peroxide), as revealed by confocal laser scanning microscopy. Additionally, sub-inhibitory concentrations of HLE disinfectant, with EDTA as an efflux pump inhibitor, inhibited the expression of multidrug EfrAB, NorE and MexCD efflux pumps in both planktonic and biofilm cultures. This could provide an alternative way to disinfect surfaces to avoid spreading multi-drug resistant strains in the food chain and the environment by decreasing efflux pump expression and consequently reducing the antibiotic selective pressure caused by systemic antibiotics and disinfectant use.
LanguageEnglish
Pages133-139
Number of pages7
JournalEnvironmental Research
Volume165
Early online date25 Apr 2018
DOIs
Publication statusPublished - 31 Aug 2018

Fingerprint

Disinfectants
inhibitor
Bacteria
pump
Biofilms
Pumps
bacterium
biofilm
EDTA
Edetic Acid
antibiotics
hydrogen peroxide
Hydrogen Peroxide
Lactic Acid
Anti-Bacterial Agents
Food Chain
acid
food chain
Confocal Microscopy
disinfectant

Keywords

  • bacterial bioilm
  • pathogens
  • disinfectant
  • antimicrobials
  • efflux pumps

Cite this

Abriouel, H., Lavilla Lerma, L., Pérez Montoro, B., Alonso, E., Knapp, C. W., Gómez, N. C., ... Benomar, N. (2018). Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria. Environmental Research, 165, 133-139. https://doi.org/10.1016/j.envres.2018.04.020
Abriouel, Hikmate ; Lavilla Lerma, Leyre ; Pérez Montoro, Beatriz ; Alonso, Esther ; Knapp, Charles W. ; Gómez, Natacha Cabellero ; Gálvez, Antonio ; Benomar, Nabil. / Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria. In: Environmental Research. 2018 ; Vol. 165. pp. 133-139.
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Abriouel, H, Lavilla Lerma, L, Pérez Montoro, B, Alonso, E, Knapp, CW, Gómez, NC, Gálvez, A & Benomar, N 2018, 'Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria' Environmental Research, vol. 165, pp. 133-139. https://doi.org/10.1016/j.envres.2018.04.020

Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria. / Abriouel, Hikmate; Lavilla Lerma, Leyre; Pérez Montoro, Beatriz; Alonso, Esther; Knapp, Charles W.; Gómez, Natacha Cabellero; Gálvez, Antonio; Benomar, Nabil.

In: Environmental Research, Vol. 165, 31.08.2018, p. 133-139.

Research output: Contribution to journalArticle

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T1 - Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria

AU - Abriouel, Hikmate

AU - Lavilla Lerma, Leyre

AU - Pérez Montoro, Beatriz

AU - Alonso, Esther

AU - Knapp, Charles W.

AU - Gómez, Natacha Cabellero

AU - Gálvez, Antonio

AU - Benomar, Nabil

PY - 2018/8/31

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N2 - We evaluated the efficacy of a new disinfectant product, HLE, to inhibit multiple species of planktonic and biofilm bacterial cultures. The HLE disinfectant comprised of EDTA, lactic acid and hydrogen peroxide, and our data indicated that the disinfectant had effective antimicrobial and anti-biofilm activity even at low concentrations (0.15% to 0.4% HLE, v/v). Furthermore, the HLE disinfectant destabilized biofilm structures eradicated them due to the synergistic effect of EDTA and both antimicrobials (lactic acid and hydrogen peroxide), as revealed by confocal laser scanning microscopy. Additionally, sub-inhibitory concentrations of HLE disinfectant, with EDTA as an efflux pump inhibitor, inhibited the expression of multidrug EfrAB, NorE and MexCD efflux pumps in both planktonic and biofilm cultures. This could provide an alternative way to disinfect surfaces to avoid spreading multi-drug resistant strains in the food chain and the environment by decreasing efflux pump expression and consequently reducing the antibiotic selective pressure caused by systemic antibiotics and disinfectant use.

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KW - pathogens

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