Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria

Hikmate Abriouel, Leyre Lavilla Lerma, Beatriz Pérez Montoro, Esther Alonso, Charles W. Knapp, Natacha Cabellero Gómez, Antonio Gálvez, Nabil Benomar

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Abstract

We evaluated the efficacy of a new disinfectant product, HLE, to inhibit multiple species of planktonic and biofilm bacterial cultures. The HLE disinfectant comprised of EDTA, lactic acid and hydrogen peroxide, and our data indicated that the disinfectant had effective antimicrobial and anti-biofilm activity even at low concentrations (0.15% to 0.4% HLE, v/v). Furthermore, the HLE disinfectant destabilized biofilm structures eradicated them due to the synergistic effect of EDTA and both antimicrobials (lactic acid and hydrogen peroxide), as revealed by confocal laser scanning microscopy. Additionally, sub-inhibitory concentrations of HLE disinfectant, with EDTA as an efflux pump inhibitor, inhibited the expression of multidrug EfrAB, NorE and MexCD efflux pumps in both planktonic and biofilm cultures. This could provide an alternative way to disinfect surfaces to avoid spreading multi-drug resistant strains in the food chain and the environment by decreasing efflux pump expression and consequently reducing the antibiotic selective pressure caused by systemic antibiotics and disinfectant use.
Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalEnvironmental Research
Volume165
Early online date25 Apr 2018
DOIs
Publication statusPublished - 31 Aug 2018

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Keywords

  • bacterial bioilm
  • pathogens
  • disinfectant
  • antimicrobials
  • efflux pumps

Cite this

Abriouel, H., Lavilla Lerma, L., Pérez Montoro, B., Alonso, E., Knapp, C. W., Gómez, N. C., ... Benomar, N. (2018). Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria. Environmental Research, 165, 133-139. https://doi.org/10.1016/j.envres.2018.04.020