Abstract
| Language | English |
|---|---|
| Pages | 133-139 |
| Number of pages | 7 |
| Journal | Environmental Research |
| Volume | 165 |
| Early online date | 25 Apr 2018 |
| DOIs | |
| Publication status | Published - 31 Aug 2018 |
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Keywords
- bacterial bioilm
- pathogens
- disinfectant
- antimicrobials
- efflux pumps
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Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria. / Abriouel, Hikmate; Lavilla Lerma, Leyre; Pérez Montoro, Beatriz; Alonso, Esther; Knapp, Charles W.; Gómez, Natacha Cabellero; Gálvez, Antonio; Benomar, Nabil.
In: Environmental Research, Vol. 165, 31.08.2018, p. 133-139.Research output: Contribution to journal › Article
TY - JOUR
T1 - Efficacy of "HLE"—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria
AU - Abriouel, Hikmate
AU - Lavilla Lerma, Leyre
AU - Pérez Montoro, Beatriz
AU - Alonso, Esther
AU - Knapp, Charles W.
AU - Gómez, Natacha Cabellero
AU - Gálvez, Antonio
AU - Benomar, Nabil
PY - 2018/8/31
Y1 - 2018/8/31
N2 - We evaluated the efficacy of a new disinfectant product, HLE, to inhibit multiple species of planktonic and biofilm bacterial cultures. The HLE disinfectant comprised of EDTA, lactic acid and hydrogen peroxide, and our data indicated that the disinfectant had effective antimicrobial and anti-biofilm activity even at low concentrations (0.15% to 0.4% HLE, v/v). Furthermore, the HLE disinfectant destabilized biofilm structures eradicated them due to the synergistic effect of EDTA and both antimicrobials (lactic acid and hydrogen peroxide), as revealed by confocal laser scanning microscopy. Additionally, sub-inhibitory concentrations of HLE disinfectant, with EDTA as an efflux pump inhibitor, inhibited the expression of multidrug EfrAB, NorE and MexCD efflux pumps in both planktonic and biofilm cultures. This could provide an alternative way to disinfect surfaces to avoid spreading multi-drug resistant strains in the food chain and the environment by decreasing efflux pump expression and consequently reducing the antibiotic selective pressure caused by systemic antibiotics and disinfectant use.
AB - We evaluated the efficacy of a new disinfectant product, HLE, to inhibit multiple species of planktonic and biofilm bacterial cultures. The HLE disinfectant comprised of EDTA, lactic acid and hydrogen peroxide, and our data indicated that the disinfectant had effective antimicrobial and anti-biofilm activity even at low concentrations (0.15% to 0.4% HLE, v/v). Furthermore, the HLE disinfectant destabilized biofilm structures eradicated them due to the synergistic effect of EDTA and both antimicrobials (lactic acid and hydrogen peroxide), as revealed by confocal laser scanning microscopy. Additionally, sub-inhibitory concentrations of HLE disinfectant, with EDTA as an efflux pump inhibitor, inhibited the expression of multidrug EfrAB, NorE and MexCD efflux pumps in both planktonic and biofilm cultures. This could provide an alternative way to disinfect surfaces to avoid spreading multi-drug resistant strains in the food chain and the environment by decreasing efflux pump expression and consequently reducing the antibiotic selective pressure caused by systemic antibiotics and disinfectant use.
KW - bacterial bioilm
KW - pathogens
KW - disinfectant
KW - antimicrobials
KW - efflux pumps
UR - https://www.sciencedirect.com/journal/environmental-research
U2 - 10.1016/j.envres.2018.04.020
DO - 10.1016/j.envres.2018.04.020
M3 - Article
VL - 165
SP - 133
EP - 139
JO - Environmental Research
T2 - Environmental Research
JF - Environmental Research
SN - 0013-9351
ER -