Efficacy of common hospital biocides with biofilms of multi-drug resistant clinical isolates

Karen Smith, I.S. Hunter

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The hospital environment is particularly susceptible to contamination by bacterial pathogens that grow on surfaces in biofilms. The effects of hospital biocides on two nosocomial pathogens, meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, growing as free-floating (planktonic) and adherent biofilm populations (sessile) were examined. Clinical isolates of MRSA and P. aeruginosa were grown as biofilms on discs of materials found in the hospital environment (stainless steel, glass, polyethylene and Teflon) and treated with three commonly used hospital biocides containing benzalkonium chloride (1 % w/v), chlorhexidine gluconate (4 % w/v) and triclosan (1 % w/v). Cell viability following biocide treatment was determined using an XTT assay and the LIVE/DEAD BacLight Bacterial Viability kit. The minimum bactericidal concentration (MBC) of all biocides for planktonic populations of both organisms was considerably less than the concentration recommended for use by the manufacturer. However, when isolates were grown as biofilms, the biocides were ineffective at killing bacteria at the concentrations recommended for use. Following biocide treatment, 0-11 % of cells in MRSA biofilms survived, and up to 80 % of cells in P. aeruginosa biofilms survived. This study suggests that although biocides may be effective against planktonic populations of bacteria, some biocides currently used in hospitals are ineffective against nosocomial pathogens growing as biofilms attached to surfaces and fail to control this reservoir for hospital-acquired infection. (Abstract from: http://jmm.sgmjournals.org/cgi/content/abstract/57/8/966)
LanguageEnglish
Pages966-973
Number of pages8
JournalJournal of medical microbiology
Volume57
Issue number8
DOIs
Publication statusPublished - Aug 2008

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Disinfectants
Biofilms
Pharmaceutical Preparations
Methicillin
Pseudomonas aeruginosa
Staphylococcus aureus
Triclosan
Population
Benzalkonium Compounds
Microbial Viability
Bacteria
Stainless Steel
Polytetrafluoroethylene
Polyethylene
Cross Infection
Glass
Cell Survival

Keywords

  • efficacy
  • common hospital biocides
  • biofilms
  • multi-drug resistant clinical isolates
  • hospital environment
  • contamination
  • bacterial pathogens

Cite this

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Efficacy of common hospital biocides with biofilms of multi-drug resistant clinical isolates. / Smith, Karen; Hunter, I.S.

In: Journal of medical microbiology, Vol. 57, No. 8, 08.2008, p. 966-973.

Research output: Contribution to journalArticle

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