Efficacy of antimicrobial 405 nm blue-light for inactivation of airborne bacteria

Research output: Contribution to conferencePoster

Abstract

Airborne transmission of infectious organisms is a considerable concern within the healthcare environment. A number of novel methods for ‘whole room’ decontamination, including antimicrobial 405 nm blue light, are being developed. To date, research has focused on its effects against surface-deposited contamination; however it is important to also establish its efficacy against airborne bacteria. This study demonstrates evidence of the dose-response kinetics of airborne bacterial contamination when exposed to 405 nm light.Bacterial aerosols of Staphylococcus epidermidis, generated using a 6-Jet Collison nebuliser, were introduced into an aerosol chamber designed to maintain prolonged airborne suspension and circulation. Aerosolized bacteria were exposed to increasing doses of 405 nm light, and air samples were extracted from the chamber using a BioSampler liquid impinger, with viability analysed using pour-plate culture. Initial results have demonstrated successful aerosol inactivation, with a 98.4% reduction achieved with 1-hour exposure to low irradiance (11.9 mWcm-2) 405 nm light (P=<0.001). Natural decay of the suspended aerosol was observed, however this was significantly less than achieved with light treatment (P=0.004). Overall, results have provided early evidence of the susceptibility of bacterial aerosols to 405 nm light. Although less germicidally efficient than UV-light, 405 nm light treatment offers benefits in terms of increased safety for human exposure, and eradication of microbes regardless of antibiotic resistance. Such benefits provide advantages for a number of applications including ‘whole room’ environmental decontamination, in which reducing levels of airborne bacteria should reduce the number of infections arising from airborne contamination.
LanguageEnglish
Number of pages1
Publication statusPublished - 27 Jan 2018
EventSPIE Photonics West 2018 BIOS - Moscone Centre , San Francisco, United States
Duration: 27 Jan 20181 Feb 2018

Conference

ConferenceSPIE Photonics West 2018 BIOS
CountryUnited States
CitySan Francisco
Period27/01/181/02/18

Fingerprint

Bacteria
Aerosols
Contamination
Decontamination
Antibiotics
Ultraviolet radiation
Dosimetry
Kinetics
Liquids
Air

Keywords

  • airborne transmission
  • infection
  • violet-blue light
  • antimicrobial properties

Cite this

Dougall, L., Anderson, J. G., MacGregor, S. J., & MacLean, M. (2018). Efficacy of antimicrobial 405 nm blue-light for inactivation of airborne bacteria. Poster session presented at SPIE Photonics West 2018 BIOS, San Francisco, United States.
Dougall, L. ; Anderson, J.G. ; MacGregor, S.J. ; MacLean, M. / Efficacy of antimicrobial 405 nm blue-light for inactivation of airborne bacteria. Poster session presented at SPIE Photonics West 2018 BIOS, San Francisco, United States.1 p.
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Dougall, L, Anderson, JG, MacGregor, SJ & MacLean, M 2018, 'Efficacy of antimicrobial 405 nm blue-light for inactivation of airborne bacteria' SPIE Photonics West 2018 BIOS, San Francisco, United States, 27/01/18 - 1/02/18, .

Efficacy of antimicrobial 405 nm blue-light for inactivation of airborne bacteria. / Dougall, L.; Anderson, J.G.; MacGregor, S.J.; MacLean, M.

2018. Poster session presented at SPIE Photonics West 2018 BIOS, San Francisco, United States.

Research output: Contribution to conferencePoster

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T1 - Efficacy of antimicrobial 405 nm blue-light for inactivation of airborne bacteria

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AU - Anderson, J.G.

AU - MacGregor, S.J.

AU - MacLean, M.

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N2 - Airborne transmission of infectious organisms is a considerable concern within the healthcare environment. A number of novel methods for ‘whole room’ decontamination, including antimicrobial 405 nm blue light, are being developed. To date, research has focused on its effects against surface-deposited contamination; however it is important to also establish its efficacy against airborne bacteria. This study demonstrates evidence of the dose-response kinetics of airborne bacterial contamination when exposed to 405 nm light.Bacterial aerosols of Staphylococcus epidermidis, generated using a 6-Jet Collison nebuliser, were introduced into an aerosol chamber designed to maintain prolonged airborne suspension and circulation. Aerosolized bacteria were exposed to increasing doses of 405 nm light, and air samples were extracted from the chamber using a BioSampler liquid impinger, with viability analysed using pour-plate culture. Initial results have demonstrated successful aerosol inactivation, with a 98.4% reduction achieved with 1-hour exposure to low irradiance (11.9 mWcm-2) 405 nm light (P=<0.001). Natural decay of the suspended aerosol was observed, however this was significantly less than achieved with light treatment (P=0.004). Overall, results have provided early evidence of the susceptibility of bacterial aerosols to 405 nm light. Although less germicidally efficient than UV-light, 405 nm light treatment offers benefits in terms of increased safety for human exposure, and eradication of microbes regardless of antibiotic resistance. Such benefits provide advantages for a number of applications including ‘whole room’ environmental decontamination, in which reducing levels of airborne bacteria should reduce the number of infections arising from airborne contamination.

AB - Airborne transmission of infectious organisms is a considerable concern within the healthcare environment. A number of novel methods for ‘whole room’ decontamination, including antimicrobial 405 nm blue light, are being developed. To date, research has focused on its effects against surface-deposited contamination; however it is important to also establish its efficacy against airborne bacteria. This study demonstrates evidence of the dose-response kinetics of airborne bacterial contamination when exposed to 405 nm light.Bacterial aerosols of Staphylococcus epidermidis, generated using a 6-Jet Collison nebuliser, were introduced into an aerosol chamber designed to maintain prolonged airborne suspension and circulation. Aerosolized bacteria were exposed to increasing doses of 405 nm light, and air samples were extracted from the chamber using a BioSampler liquid impinger, with viability analysed using pour-plate culture. Initial results have demonstrated successful aerosol inactivation, with a 98.4% reduction achieved with 1-hour exposure to low irradiance (11.9 mWcm-2) 405 nm light (P=<0.001). Natural decay of the suspended aerosol was observed, however this was significantly less than achieved with light treatment (P=0.004). Overall, results have provided early evidence of the susceptibility of bacterial aerosols to 405 nm light. Although less germicidally efficient than UV-light, 405 nm light treatment offers benefits in terms of increased safety for human exposure, and eradication of microbes regardless of antibiotic resistance. Such benefits provide advantages for a number of applications including ‘whole room’ environmental decontamination, in which reducing levels of airborne bacteria should reduce the number of infections arising from airborne contamination.

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KW - infection

KW - violet-blue light

KW - antimicrobial properties

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M3 - Poster

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Dougall L, Anderson JG, MacGregor SJ, MacLean M. Efficacy of antimicrobial 405 nm blue-light for inactivation of airborne bacteria. 2018. Poster session presented at SPIE Photonics West 2018 BIOS, San Francisco, United States.