Efficacy of 405nm light for inactivation of feline calicivirus: a surrogate for norovirus

Rachael Tomb, Michelle MacLean, John Coia, Scott MacGregor, Libby Graham, Mike MacDonald, John Anderson

Research output: Contribution to conferencePoster

Abstract

Background: Norovirus (NoV) is a leading cause of nosocomial gastroenteritis. With only 10-100 viral particles required for infection, effective decontamination of hospitals is particularly important in preventing the spread of this highly transmissible virus. A technology for continuous environmental decontamination has been developed which utilises 405nm light to inactivate bacteria and fungi, via ROS-generated oxidative damage.

Aim(s)/Objectives: To date, the antiviral efficacy of 405nm light has not been fully determined, therefore this study was designed to investigate whether feline calicivirus (FCV), the standard surrogate for NoV, can be inactivated by 405nm light, and how viral susceptibility may be influenced when suspended in biologically-relevant material.

Method: FCV was exposed to 405nm light (155.8mW/cm2) whilst suspended in various minimal andnutrient-rich media and inactivation curves were used to establish sensitivity. Viralin activation was measured by assessing infection of a feline embryo cell line (FEA).

Results: Antiviral activity of 405nm light against FCV was successfully demonstrated, with a 4-log10(99.99%) reduction in minimal media evident after a dose of 2.8kJ/cm2. However susceptibility was significantly enhanced when exposed in nutrient-rich DMEM and artificial saliva, with an approximate 5-fold reduction in dose required (420J/cm2)for an equivalent level of inactivation.

Discussion & Conclusion: The results indicate that FCV can be inactivated using 405nm light, and susceptibility can be significantly enhanced when the virus is present in nutrient-rich, or biologically-relevant material such as saliva. Further research in this area could lead to the development of 405nm light technology for effective NoV decontamination within the hospital environment.

Conference

ConferenceThe 9th Healthcare Infection Society International Conference 2014
Abbreviated titleHIS2014
CountryFrance
CityLyon
Period16/11/1418/11/14
Internet address

Fingerprint

Feline Calicivirus
Feline calicivirus
Norovirus
inactivation
Light
Decontamination
decontamination
saliva
Antiviral Agents
Artificial Saliva
Viruses
Technology
Food
viruses
Felidae
gastroenteritis
Gastroenteritis
nutrients
dosage
Infection

Keywords

  • 405nm light
  • feline calicivirus
  • antiviral activity
  • decontamination

Cite this

Tomb, R., MacLean, M., Coia, J., MacGregor, S., Graham, L., MacDonald, M., & Anderson, J. (2014). Efficacy of 405nm light for inactivation of feline calicivirus: a surrogate for norovirus. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.
Tomb, Rachael ; MacLean, Michelle ; Coia, John ; MacGregor, Scott ; Graham, Libby ; MacDonald, Mike ; Anderson, John. / Efficacy of 405nm light for inactivation of feline calicivirus : a surrogate for norovirus. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.
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title = "Efficacy of 405nm light for inactivation of feline calicivirus: a surrogate for norovirus",
abstract = "Background: Norovirus (NoV) is a leading cause of nosocomial gastroenteritis. With only 10-100 viral particles required for infection, effective decontamination of hospitals is particularly important in preventing the spread of this highly transmissible virus. A technology for continuous environmental decontamination has been developed which utilises 405nm light to inactivate bacteria and fungi, via ROS-generated oxidative damage.Aim(s)/Objectives: To date, the antiviral efficacy of 405nm light has not been fully determined, therefore this study was designed to investigate whether feline calicivirus (FCV), the standard surrogate for NoV, can be inactivated by 405nm light, and how viral susceptibility may be influenced when suspended in biologically-relevant material.Method: FCV was exposed to 405nm light (155.8mW/cm2) whilst suspended in various minimal andnutrient-rich media and inactivation curves were used to establish sensitivity. Viralin activation was measured by assessing infection of a feline embryo cell line (FEA).Results: Antiviral activity of 405nm light against FCV was successfully demonstrated, with a 4-log10(99.99{\%}) reduction in minimal media evident after a dose of 2.8kJ/cm2. However susceptibility was significantly enhanced when exposed in nutrient-rich DMEM and artificial saliva, with an approximate 5-fold reduction in dose required (420J/cm2)for an equivalent level of inactivation.Discussion & Conclusion: The results indicate that FCV can be inactivated using 405nm light, and susceptibility can be significantly enhanced when the virus is present in nutrient-rich, or biologically-relevant material such as saliva. Further research in this area could lead to the development of 405nm light technology for effective NoV decontamination within the hospital environment.",
keywords = "405nm light, feline calicivirus, antiviral activity, decontamination",
author = "Rachael Tomb and Michelle MacLean and John Coia and Scott MacGregor and Libby Graham and Mike MacDonald and John Anderson",
year = "2014",
month = "11",
language = "English",
note = "The 9th Healthcare Infection Society International Conference 2014, HIS2014 ; Conference date: 16-11-2014 Through 18-11-2014",
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Tomb, R, MacLean, M, Coia, J, MacGregor, S, Graham, L, MacDonald, M & Anderson, J 2014, 'Efficacy of 405nm light for inactivation of feline calicivirus: a surrogate for norovirus' The 9th Healthcare Infection Society International Conference 2014, Lyon, France, 16/11/14 - 18/11/14, .

Efficacy of 405nm light for inactivation of feline calicivirus : a surrogate for norovirus. / Tomb, Rachael ; MacLean, Michelle; Coia, John; MacGregor, Scott; Graham, Libby; MacDonald, Mike; Anderson, John.

2014. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Efficacy of 405nm light for inactivation of feline calicivirus

T2 - a surrogate for norovirus

AU - Tomb, Rachael

AU - MacLean, Michelle

AU - Coia, John

AU - MacGregor, Scott

AU - Graham, Libby

AU - MacDonald, Mike

AU - Anderson, John

PY - 2014/11

Y1 - 2014/11

N2 - Background: Norovirus (NoV) is a leading cause of nosocomial gastroenteritis. With only 10-100 viral particles required for infection, effective decontamination of hospitals is particularly important in preventing the spread of this highly transmissible virus. A technology for continuous environmental decontamination has been developed which utilises 405nm light to inactivate bacteria and fungi, via ROS-generated oxidative damage.Aim(s)/Objectives: To date, the antiviral efficacy of 405nm light has not been fully determined, therefore this study was designed to investigate whether feline calicivirus (FCV), the standard surrogate for NoV, can be inactivated by 405nm light, and how viral susceptibility may be influenced when suspended in biologically-relevant material.Method: FCV was exposed to 405nm light (155.8mW/cm2) whilst suspended in various minimal andnutrient-rich media and inactivation curves were used to establish sensitivity. Viralin activation was measured by assessing infection of a feline embryo cell line (FEA).Results: Antiviral activity of 405nm light against FCV was successfully demonstrated, with a 4-log10(99.99%) reduction in minimal media evident after a dose of 2.8kJ/cm2. However susceptibility was significantly enhanced when exposed in nutrient-rich DMEM and artificial saliva, with an approximate 5-fold reduction in dose required (420J/cm2)for an equivalent level of inactivation.Discussion & Conclusion: The results indicate that FCV can be inactivated using 405nm light, and susceptibility can be significantly enhanced when the virus is present in nutrient-rich, or biologically-relevant material such as saliva. Further research in this area could lead to the development of 405nm light technology for effective NoV decontamination within the hospital environment.

AB - Background: Norovirus (NoV) is a leading cause of nosocomial gastroenteritis. With only 10-100 viral particles required for infection, effective decontamination of hospitals is particularly important in preventing the spread of this highly transmissible virus. A technology for continuous environmental decontamination has been developed which utilises 405nm light to inactivate bacteria and fungi, via ROS-generated oxidative damage.Aim(s)/Objectives: To date, the antiviral efficacy of 405nm light has not been fully determined, therefore this study was designed to investigate whether feline calicivirus (FCV), the standard surrogate for NoV, can be inactivated by 405nm light, and how viral susceptibility may be influenced when suspended in biologically-relevant material.Method: FCV was exposed to 405nm light (155.8mW/cm2) whilst suspended in various minimal andnutrient-rich media and inactivation curves were used to establish sensitivity. Viralin activation was measured by assessing infection of a feline embryo cell line (FEA).Results: Antiviral activity of 405nm light against FCV was successfully demonstrated, with a 4-log10(99.99%) reduction in minimal media evident after a dose of 2.8kJ/cm2. However susceptibility was significantly enhanced when exposed in nutrient-rich DMEM and artificial saliva, with an approximate 5-fold reduction in dose required (420J/cm2)for an equivalent level of inactivation.Discussion & Conclusion: The results indicate that FCV can be inactivated using 405nm light, and susceptibility can be significantly enhanced when the virus is present in nutrient-rich, or biologically-relevant material such as saliva. Further research in this area could lead to the development of 405nm light technology for effective NoV decontamination within the hospital environment.

KW - 405nm light

KW - feline calicivirus

KW - antiviral activity

KW - decontamination

UR - http://www.his.org.uk/events/his2014/

UR - http://www.his.org.uk/files/7414/1535/3959/Poster_abstracts_for_HIS_Website_Nov_2014.pdf

M3 - Poster

ER -

Tomb R, MacLean M, Coia J, MacGregor S, Graham L, MacDonald M et al. Efficacy of 405nm light for inactivation of feline calicivirus: a surrogate for norovirus. 2014. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.