Effects of sterilisation method on surface topography and in-vitro cell behaviour of electrostatically spun scaffolds

K.D. Andrews, J.A. Hunt, R.A. Black

    Research output: Contribution to journalArticle

    46 Citations (Scopus)

    Abstract

    Electrostatic spinning is a potentially significant technique for scaffold production within the field of tissue engineering; however, the effect of sterilisation upon these structures is not known. This research investigated the extent of any topographical alteration to electrostatically spun scaffolds post-production through sterilisation, and examined any subsequent effect on contacting cells. Scaffolds made from Tecoflex® SG-80A polyurethane were sterilised using ethylene oxide and UV-ozone. Scaffold topography was characterized in terms of inter-fibre separation (ifs), fibre diameter (f.dia) and surface roughness. Cell culture was performed over 7 days with both mouse L929 and human embryonic lung fibroblasts, the results of which were assessed using SEM, image analysis and confocal microscopy. Sterilisation by UV-ozone and ethylene oxide decreased ifs and increased f.dia; surface roughness was decreased by UV-ozone but increased by ethylene oxide. Possible mechanisms to explain these observations are discussed, namely photo-oxidative degradation in the case of UV-ozone and process-induced changes in surface roughness. UV-ozone sterilised scaffolds showed greater cell coverage than those treated with ethylene oxide, but lower coverage than all the controls. Changes in cell attachment and morphology were thought to be due to the changes in topography brought about by the sterilisation process. We conclude that surface modification by sterilisation could prove to be a useful tool at the final stage of scaffold production to enhance cell contact, phenotype or function.
    LanguageEnglish
    Pages1014-1026
    Number of pages12
    JournalBiomaterials
    Volume28
    Issue number6
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Ozone
    Surface topography
    Ethylene Oxide
    Scaffolds
    Ethylene
    Oxides
    Fibers
    Surface roughness
    Topography
    Polyurethanes
    Confocal microscopy
    Fibroblasts
    Tissue Engineering
    Scaffolds (biology)
    Static Electricity
    Tissue engineering
    Cell culture
    Confocal Microscopy
    Image analysis
    Surface treatment

    Keywords

    • cell adhesion
    • fibroblast
    • polyurethane
    • scaffold
    • sterilisation
    • surface topography
    • bioengineering

    Cite this

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    Effects of sterilisation method on surface topography and in-vitro cell behaviour of electrostatically spun scaffolds. / Andrews, K.D.; Hunt, J.A.; Black, R.A.

    In: Biomaterials, Vol. 28, No. 6, 2007, p. 1014-1026.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Andrews, K.D.

    AU - Hunt, J.A.

    AU - Black, R.A.

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    AB - Electrostatic spinning is a potentially significant technique for scaffold production within the field of tissue engineering; however, the effect of sterilisation upon these structures is not known. This research investigated the extent of any topographical alteration to electrostatically spun scaffolds post-production through sterilisation, and examined any subsequent effect on contacting cells. Scaffolds made from Tecoflex® SG-80A polyurethane were sterilised using ethylene oxide and UV-ozone. Scaffold topography was characterized in terms of inter-fibre separation (ifs), fibre diameter (f.dia) and surface roughness. Cell culture was performed over 7 days with both mouse L929 and human embryonic lung fibroblasts, the results of which were assessed using SEM, image analysis and confocal microscopy. Sterilisation by UV-ozone and ethylene oxide decreased ifs and increased f.dia; surface roughness was decreased by UV-ozone but increased by ethylene oxide. Possible mechanisms to explain these observations are discussed, namely photo-oxidative degradation in the case of UV-ozone and process-induced changes in surface roughness. UV-ozone sterilised scaffolds showed greater cell coverage than those treated with ethylene oxide, but lower coverage than all the controls. Changes in cell attachment and morphology were thought to be due to the changes in topography brought about by the sterilisation process. We conclude that surface modification by sterilisation could prove to be a useful tool at the final stage of scaffold production to enhance cell contact, phenotype or function.

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