Effects of purified cardiotoxins from the Thailand cobra (Naja naja siamensis) on isolated skeletal and cardiac muscle preparations

A L Harvey, R J Marshall, E Karlsson

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Four polypeptides were isolated by non-exchange chromatography from the crude cardiotoxin fraction of Thailand cobra (Naja naja siamensis) venom. Fraction I and 71 amino acid residues including 1 tryptophan, while fractions, II, III and IV had 60 amino acids and no tryptophan. When tested on isolated skeletal muscle (chick biventer cervicis, chick embryo muscle in culture, guinea pig hemidiaphragm) and cardiac muscle (guinea pig and cat left atria, cat papillary muscle) preparations, fractions II, III and IV, but not fraction I, caused contracture and depolarization. The cardiotoxin-induced contractions could be prevented by pretreatment with raised concentrations of calcium, but were not influenced by a wide range of pharmacological agents which modify nerve-muscle transmission or muscle contractility. The results suggest that cardiotoxins do not act at a specific step in normal excitation-contraction coupling but directly on cell membranes, where they probably cause the formation of pores which result in depolarization and in the influx of calcium.
LanguageEnglish
Pages379-396
Number of pages18
JournalToxicon
Volume20
Issue number2
DOIs
Publication statusPublished - 1982

Fingerprint

Cobra Cardiotoxin Proteins
Cardiotoxins
Elapidae
Thailand
Muscle
Myocardium
Skeletal Muscle
Tryptophan
Muscles
Guinea Pigs
Cats
Calcium
Cobra Venoms
Amino Acids
Excitation Contraction Coupling
Papillary Muscles
Depolarization
Contracture
Chick Embryo
Heart Atria

Keywords

  • amino acids
  • animals
  • calcium
  • cats
  • chick embryo
  • chickens
  • cobra cardiotoxin proteins
  • cobra venoms
  • female
  • guinea pigs
  • heart
  • male
  • membrane potentials
  • muscle contraction
  • muscles
  • myocardial contraction
  • Snakes

Cite this

@article{f2f0915c9a0f4d948af1b5af683ce897,
title = "Effects of purified cardiotoxins from the Thailand cobra (Naja naja siamensis) on isolated skeletal and cardiac muscle preparations",
abstract = "Four polypeptides were isolated by non-exchange chromatography from the crude cardiotoxin fraction of Thailand cobra (Naja naja siamensis) venom. Fraction I and 71 amino acid residues including 1 tryptophan, while fractions, II, III and IV had 60 amino acids and no tryptophan. When tested on isolated skeletal muscle (chick biventer cervicis, chick embryo muscle in culture, guinea pig hemidiaphragm) and cardiac muscle (guinea pig and cat left atria, cat papillary muscle) preparations, fractions II, III and IV, but not fraction I, caused contracture and depolarization. The cardiotoxin-induced contractions could be prevented by pretreatment with raised concentrations of calcium, but were not influenced by a wide range of pharmacological agents which modify nerve-muscle transmission or muscle contractility. The results suggest that cardiotoxins do not act at a specific step in normal excitation-contraction coupling but directly on cell membranes, where they probably cause the formation of pores which result in depolarization and in the influx of calcium.",
keywords = "amino acids, animals, calcium, cats, chick embryo, chickens, cobra cardiotoxin proteins, cobra venoms, female, guinea pigs, heart, male, membrane potentials, muscle contraction, muscles, myocardial contraction, Snakes",
author = "Harvey, {A L} and Marshall, {R J} and E Karlsson",
year = "1982",
doi = "10.1016/0041-0101(82)90001-0",
language = "English",
volume = "20",
pages = "379--396",
journal = "Toxicon",
issn = "0041-0101",
number = "2",

}

Effects of purified cardiotoxins from the Thailand cobra (Naja naja siamensis) on isolated skeletal and cardiac muscle preparations. / Harvey, A L; Marshall, R J; Karlsson, E.

In: Toxicon, Vol. 20, No. 2, 1982, p. 379-396.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of purified cardiotoxins from the Thailand cobra (Naja naja siamensis) on isolated skeletal and cardiac muscle preparations

AU - Harvey, A L

AU - Marshall, R J

AU - Karlsson, E

PY - 1982

Y1 - 1982

N2 - Four polypeptides were isolated by non-exchange chromatography from the crude cardiotoxin fraction of Thailand cobra (Naja naja siamensis) venom. Fraction I and 71 amino acid residues including 1 tryptophan, while fractions, II, III and IV had 60 amino acids and no tryptophan. When tested on isolated skeletal muscle (chick biventer cervicis, chick embryo muscle in culture, guinea pig hemidiaphragm) and cardiac muscle (guinea pig and cat left atria, cat papillary muscle) preparations, fractions II, III and IV, but not fraction I, caused contracture and depolarization. The cardiotoxin-induced contractions could be prevented by pretreatment with raised concentrations of calcium, but were not influenced by a wide range of pharmacological agents which modify nerve-muscle transmission or muscle contractility. The results suggest that cardiotoxins do not act at a specific step in normal excitation-contraction coupling but directly on cell membranes, where they probably cause the formation of pores which result in depolarization and in the influx of calcium.

AB - Four polypeptides were isolated by non-exchange chromatography from the crude cardiotoxin fraction of Thailand cobra (Naja naja siamensis) venom. Fraction I and 71 amino acid residues including 1 tryptophan, while fractions, II, III and IV had 60 amino acids and no tryptophan. When tested on isolated skeletal muscle (chick biventer cervicis, chick embryo muscle in culture, guinea pig hemidiaphragm) and cardiac muscle (guinea pig and cat left atria, cat papillary muscle) preparations, fractions II, III and IV, but not fraction I, caused contracture and depolarization. The cardiotoxin-induced contractions could be prevented by pretreatment with raised concentrations of calcium, but were not influenced by a wide range of pharmacological agents which modify nerve-muscle transmission or muscle contractility. The results suggest that cardiotoxins do not act at a specific step in normal excitation-contraction coupling but directly on cell membranes, where they probably cause the formation of pores which result in depolarization and in the influx of calcium.

KW - amino acids

KW - animals

KW - calcium

KW - cats

KW - chick embryo

KW - chickens

KW - cobra cardiotoxin proteins

KW - cobra venoms

KW - female

KW - guinea pigs

KW - heart

KW - male

KW - membrane potentials

KW - muscle contraction

KW - muscles

KW - myocardial contraction

KW - Snakes

U2 - 10.1016/0041-0101(82)90001-0

DO - 10.1016/0041-0101(82)90001-0

M3 - Article

VL - 20

SP - 379

EP - 396

JO - Toxicon

T2 - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 2

ER -