Effects of fasciculin 2, an anticholinesterase polypeptide from green mamba venom, on neuromuscular transmission in mouse diaphgragm preparations

A.J. Anderson, A. L. Harvey, P. M. Mbugua

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fasciculin 2, a polypeptide from green mamba (Dendroaspis angusticeps) venorn, causes an increase in the twitch response of mouse phrenic nerve-hemidiaphragm preparations to indirect stimulation. Intracellular recording reveals that fasciculin 2 augments neuromuscular transmission by increasing the amplitude and duration of endplate potentials. Its action is not reversed by washing. Interactions with neostigmine confirm that fasciculin 2 acts as an anticholinesterase. It has no presynaptic actions on transmitter release or postsynaptic receptor blocking actions. On chicken muscle preparations, fasciculin 2 has no anticholinesterase actions. Because of this selectivity and its apparent irreversibility, fasciculin 2 should be useful in characterizing different forms of acetylcholinesterase.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalNeuroscience Letters
Volume54
Issue number2-3
DOIs
Publication statusPublished - 1 Jan 1985

Keywords

  • anticholinesterase
  • cultured muscle
  • green mamba
  • mouse
  • neuromuscular transmission
  • snake toxins
  • fasciculin 2

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