Effects of eutrophication on vitellogenin gene expression in male fathead minnows (pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms

D.A. Gordan, G.P. Toth, D.W. Graham, J.A. Lazorchak, T.V. Redd, Charles W. Knapp, F. deNoyelles, Scott Campbell, D.L. Lattier

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10 Citations (Scopus)

Abstract

This study evaluated the effect of aquatic secondary nutrient supply levels (nitrogen and phosphorus) on the subcellular response of adult male fathead minnows (Pimephales promelas) exposed to a single nominal concentration of 17 alpha-ethynytestradiol (EE2), a potent synthetic estrogen, under quasi-natural field conditions. Outdoor mesocosms were maintained under low, medium, and high nutrient supply conditions as categorized by total phosphorus (TP) level (nominal 0.012, 0.025, and 0.045 mg TP/L, respectively), and treated with EE2 with and without a carrier solvent. Using reverse transcription-polymerase chain reaction methods, vitellogenin gene (Vg) expression was determined in the fish collected at 0 h, 8 h, 24 h, 4 d, 7 d, and 14 d post-exposure. Induction of Vg was detected as early as 8 It post-exposure, with and without the carrier solvent, and persisted through Day 14. Results showed Vg to be significantly greater at low nutrient levels (p < 0.05), suggesting that EE2 bioavailability to the fish was likely greater under less-turbid water conditions. Published by Elsevier Ltd.
Original languageEnglish
Pages (from-to)559-566
Number of pages8
JournalEnvironmental Pollution
Volume142
Issue number3
DOIs
Publication statusPublished - Aug 2006

Keywords

  • nutrient enrichment
  • 17 alpha-ethynylestradiol
  • fathead minnow
  • vitellogenin
  • mesocosms

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