Effects of CoCr metal wear debris generated from metal-on-metal hip implants and Co ions on human monocyte-like U937 cells

Olga M. Posada, Rothwelle J. Tate, M. Helen Grant

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Hip resurfacing with cobalt–chromium (CoCr) alloy was developed as a surgical alternative to total hip replacement. However, the biological effects of nanoparticles generated by wear at the metal-on-metal articulating surfaces has limited the success of such implants. The aim of this study was to investigate the effects of the combined exposure to CoCr nanoparticles and cobalt ions released from a resurfacing implant on monocytes (U937 cells) and whether these resulted in morphology changes, proliferation alterations, toxicity and cytokine release. The interaction between prior exposure to Co ions and the cellular response to nanoparticulate debris was determined to simulate the situation in patients with metal-on-metal implants receiving a second implant. Effects on U937 cells were mainly seen after 120 h of treatment. Prior exposure to Co ions increased the toxic effects induced by the debris, and by Co ions themselves, suggesting the potential for interaction in vivo. Increased TNF-α secretion by resting cells exposed to nanoparticles could contribute to osteolysis processes in vivo, while increased IFN-γ production by activated cells could represent cellular protection against tissue damage. Data suggest that interactions between Co ions and CoCr nanoparticles would occur in vivo, and could threaten the survival of a CoCr metal implant.
LanguageEnglish
Pages271-280
Number of pages10
JournalToxicology in Vitro
Volume29
Issue number2
Early online date27 Nov 2014
DOIs
Publication statusPublished - Mar 2015

Fingerprint

U937 Cells
Chromium
Cobalt
Debris
Hip
Monocytes
Metals
Nanoparticles
Wear of materials
Ions
Chromium Alloys
Osteolysis
Hip Replacement Arthroplasties
Poisons
Toxicity
Tumor Necrosis Factor-alpha
Tissue
Cytokines
Survival

Keywords

  • metal wear debris
  • hip replacements
  • nanoparticles
  • metal ions
  • cobalt-chrome alloy
  • monocytes

Cite this

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abstract = "Hip resurfacing with cobalt–chromium (CoCr) alloy was developed as a surgical alternative to total hip replacement. However, the biological effects of nanoparticles generated by wear at the metal-on-metal articulating surfaces has limited the success of such implants. The aim of this study was to investigate the effects of the combined exposure to CoCr nanoparticles and cobalt ions released from a resurfacing implant on monocytes (U937 cells) and whether these resulted in morphology changes, proliferation alterations, toxicity and cytokine release. The interaction between prior exposure to Co ions and the cellular response to nanoparticulate debris was determined to simulate the situation in patients with metal-on-metal implants receiving a second implant. Effects on U937 cells were mainly seen after 120 h of treatment. Prior exposure to Co ions increased the toxic effects induced by the debris, and by Co ions themselves, suggesting the potential for interaction in vivo. Increased TNF-α secretion by resting cells exposed to nanoparticles could contribute to osteolysis processes in vivo, while increased IFN-γ production by activated cells could represent cellular protection against tissue damage. Data suggest that interactions between Co ions and CoCr nanoparticles would occur in vivo, and could threaten the survival of a CoCr metal implant.",
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Effects of CoCr metal wear debris generated from metal-on-metal hip implants and Co ions on human monocyte-like U937 cells. / Posada, Olga M.; Tate, Rothwelle J.; Grant, M. Helen.

In: Toxicology in Vitro, Vol. 29, No. 2, 03.2015, p. 271-280.

Research output: Contribution to journalArticle

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