Effects of adjuvant, dose and carrier pre-sensitisation on the immunisation efficacy of a GnRH analogue

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A GnRH-neutralising vaccine, with potential applications in the treatment of human sex hormone-dependent disorders, was developed by conjugating GnRH-glycys to tetanus toxoid. An evaluation of adjuvant, dose and carrier pre-sensitisation was made. Male rats immunised with the conjugate, adsorbed onto alum, showed higher anti-GnRH antibody levels and suppressed testosterone concentrations, compared with animals immunised without adjuvant. Conjugate administration in a four injection regime proved to be the most effective in disrupting fertility, as assessed by the degree of lowered testosterone levels and gonadal atrophy. Pre-sensitisation with tetanus toxoid had an initial marked effect on immunisation, observed following 2 drug doses; the pre-sensitised animals showed a lower antibody response to the conjugate than did the non-primed animals. However, as the number of drug doses increased to 4, there was no significant difference between the primed and non-primed animals.

LanguageEnglish
Pages179-195
Number of pages17
JournalDrug Design and Discovery
Volume14
Issue number3
Publication statusPublished - 31 Dec 1996

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Gonadotropin-Releasing Hormone
Immunization
Tetanus Toxoid
Testosterone
Gonadal Steroid Hormones
Pharmaceutical Preparations
Atrophy
Antibody Formation
Fertility
Anti-Idiotypic Antibodies
Vaccines
Injections
Therapeutics

Keywords

  • adjuvants
  • contraceptive agent
  • gonadorelin derivative
  • testosterone
  • tetanus toxoid
  • vaccine

Cite this

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Effects of adjuvant, dose and carrier pre-sensitisation on the immunisation efficacy of a GnRH analogue. / Ferro, V A; Stimson, W H.

In: Drug Design and Discovery, Vol. 14, No. 3, 31.12.1996, p. 179-195.

Research output: Contribution to journalArticle

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