Effect of visible light exposure on e. coli treated with pulsed UV-rich light

Y. Lamont, S.J. MacGregor, J.G. Anderson, R.A. Fouracre

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

Despite the significant advances that have been made toward a better understanding of microbial transmission and pathogenicity, the levels of food/water-borne infection still remain high. Concern over this situation has led to a search for alternative technologies including the use of pulsed power applications, such as pulsed UV-rich light treatment, for sterilisation and disinfection purposes. Pulsed Ultra Violet (PUV) treatment involves the use of intense and short-duration pulses of UV-rich light to inactivate microorganisms. This method has an advantage over traditional UV delivery systems where applications are limited due to the long exposure times required with the low intensity emissions which are produced. The antimicrobial effect of pulsed light is primarily due to the ultraviolet content, which causes transformations of the pyrimidine bases of microbial nucleic acids. However, a drawback with pulsed light treatment is that many microorganisms are known to possess photo-repair mechanisms that facilitate recovery following sub-lethal exposure to continuous UV rich light. Both light and dark repair mechanisms are known to exist, but the extent to which microorganisms can exhibit photo-repair following pulsed light exposure is not clear. Experiments were carried out in which E. coli was exposed to pulsed UV-rich light from a low-pressure (450 torr) xenon flashlamp (Samtech Ltd). Following exposure to pulsed light, the treated cells were kept for varying periods, either in light (various intensities) or in dark conditions, and the surviving populations enumerated. Results showed little evidence of dark repair, whereas substantial photo-repair took place (2.1 log increase) when cells were exposed to visible light following treatment. The results also show that when the intensity of visible light is increased the greater the level of photoreactivation that occurs.

Conference

Conference26th International Power Modulator Symposium, 2004 and 2004 High-Voltage Workshop
CountryUnited Kingdom
Period23/05/0426/05/04

Fingerprint

microorganisms
nucleic acids
pyrimidines
infectious diseases
cells
food
xenon
luminous intensity
delivery
pulse duration
low pressure
recovery
causes
water

Keywords

  • effect
  • visible light exposure
  • e.coli treated
  • pulsed
  • uv-rich light
  • DNA
  • xenon
  • wastewater treatment
  • surface treatment
  • surface contamination
  • pathogens
  • packaging
  • microorganisms
  • food technology
  • diseases

Cite this

Lamont, Y., MacGregor, S. J., Anderson, J. G., & Fouracre, R. A. (2004). Effect of visible light exposure on e. coli treated with pulsed UV-rich light. Paper presented at 26th International Power Modulator Symposium, 2004 and 2004 High-Voltage Workshop , United Kingdom. https://doi.org/10.1109/MODSYM.2004.1433653
Lamont, Y. ; MacGregor, S.J. ; Anderson, J.G. ; Fouracre, R.A. / Effect of visible light exposure on e. coli treated with pulsed UV-rich light. Paper presented at 26th International Power Modulator Symposium, 2004 and 2004 High-Voltage Workshop , United Kingdom.4 p.
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abstract = "Despite the significant advances that have been made toward a better understanding of microbial transmission and pathogenicity, the levels of food/water-borne infection still remain high. Concern over this situation has led to a search for alternative technologies including the use of pulsed power applications, such as pulsed UV-rich light treatment, for sterilisation and disinfection purposes. Pulsed Ultra Violet (PUV) treatment involves the use of intense and short-duration pulses of UV-rich light to inactivate microorganisms. This method has an advantage over traditional UV delivery systems where applications are limited due to the long exposure times required with the low intensity emissions which are produced. The antimicrobial effect of pulsed light is primarily due to the ultraviolet content, which causes transformations of the pyrimidine bases of microbial nucleic acids. However, a drawback with pulsed light treatment is that many microorganisms are known to possess photo-repair mechanisms that facilitate recovery following sub-lethal exposure to continuous UV rich light. Both light and dark repair mechanisms are known to exist, but the extent to which microorganisms can exhibit photo-repair following pulsed light exposure is not clear. Experiments were carried out in which E. coli was exposed to pulsed UV-rich light from a low-pressure (450 torr) xenon flashlamp (Samtech Ltd). Following exposure to pulsed light, the treated cells were kept for varying periods, either in light (various intensities) or in dark conditions, and the surviving populations enumerated. Results showed little evidence of dark repair, whereas substantial photo-repair took place (2.1 log increase) when cells were exposed to visible light following treatment. The results also show that when the intensity of visible light is increased the greater the level of photoreactivation that occurs.",
keywords = "effect, visible light exposure, e.coli treated, pulsed, uv-rich light, DNA, xenon , wastewater treatment, surface treatment, surface contamination, pathogens, packaging, microorganisms, food technology , diseases",
author = "Y. Lamont and S.J. MacGregor and J.G. Anderson and R.A. Fouracre",
year = "2004",
month = "5",
doi = "10.1109/MODSYM.2004.1433653",
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Lamont, Y, MacGregor, SJ, Anderson, JG & Fouracre, RA 2004, 'Effect of visible light exposure on e. coli treated with pulsed UV-rich light' Paper presented at 26th International Power Modulator Symposium, 2004 and 2004 High-Voltage Workshop , United Kingdom, 23/05/04 - 26/05/04, . https://doi.org/10.1109/MODSYM.2004.1433653

Effect of visible light exposure on e. coli treated with pulsed UV-rich light. / Lamont, Y.; MacGregor, S.J.; Anderson, J.G.; Fouracre, R.A.

2004. Paper presented at 26th International Power Modulator Symposium, 2004 and 2004 High-Voltage Workshop , United Kingdom.

Research output: Contribution to conferencePaper

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T1 - Effect of visible light exposure on e. coli treated with pulsed UV-rich light

AU - Lamont, Y.

AU - MacGregor, S.J.

AU - Anderson, J.G.

AU - Fouracre, R.A.

PY - 2004/5

Y1 - 2004/5

N2 - Despite the significant advances that have been made toward a better understanding of microbial transmission and pathogenicity, the levels of food/water-borne infection still remain high. Concern over this situation has led to a search for alternative technologies including the use of pulsed power applications, such as pulsed UV-rich light treatment, for sterilisation and disinfection purposes. Pulsed Ultra Violet (PUV) treatment involves the use of intense and short-duration pulses of UV-rich light to inactivate microorganisms. This method has an advantage over traditional UV delivery systems where applications are limited due to the long exposure times required with the low intensity emissions which are produced. The antimicrobial effect of pulsed light is primarily due to the ultraviolet content, which causes transformations of the pyrimidine bases of microbial nucleic acids. However, a drawback with pulsed light treatment is that many microorganisms are known to possess photo-repair mechanisms that facilitate recovery following sub-lethal exposure to continuous UV rich light. Both light and dark repair mechanisms are known to exist, but the extent to which microorganisms can exhibit photo-repair following pulsed light exposure is not clear. Experiments were carried out in which E. coli was exposed to pulsed UV-rich light from a low-pressure (450 torr) xenon flashlamp (Samtech Ltd). Following exposure to pulsed light, the treated cells were kept for varying periods, either in light (various intensities) or in dark conditions, and the surviving populations enumerated. Results showed little evidence of dark repair, whereas substantial photo-repair took place (2.1 log increase) when cells were exposed to visible light following treatment. The results also show that when the intensity of visible light is increased the greater the level of photoreactivation that occurs.

AB - Despite the significant advances that have been made toward a better understanding of microbial transmission and pathogenicity, the levels of food/water-borne infection still remain high. Concern over this situation has led to a search for alternative technologies including the use of pulsed power applications, such as pulsed UV-rich light treatment, for sterilisation and disinfection purposes. Pulsed Ultra Violet (PUV) treatment involves the use of intense and short-duration pulses of UV-rich light to inactivate microorganisms. This method has an advantage over traditional UV delivery systems where applications are limited due to the long exposure times required with the low intensity emissions which are produced. The antimicrobial effect of pulsed light is primarily due to the ultraviolet content, which causes transformations of the pyrimidine bases of microbial nucleic acids. However, a drawback with pulsed light treatment is that many microorganisms are known to possess photo-repair mechanisms that facilitate recovery following sub-lethal exposure to continuous UV rich light. Both light and dark repair mechanisms are known to exist, but the extent to which microorganisms can exhibit photo-repair following pulsed light exposure is not clear. Experiments were carried out in which E. coli was exposed to pulsed UV-rich light from a low-pressure (450 torr) xenon flashlamp (Samtech Ltd). Following exposure to pulsed light, the treated cells were kept for varying periods, either in light (various intensities) or in dark conditions, and the surviving populations enumerated. Results showed little evidence of dark repair, whereas substantial photo-repair took place (2.1 log increase) when cells were exposed to visible light following treatment. The results also show that when the intensity of visible light is increased the greater the level of photoreactivation that occurs.

KW - effect

KW - visible light exposure

KW - e.coli treated

KW - pulsed

KW - uv-rich light

KW - DNA

KW - xenon

KW - wastewater treatment

KW - surface treatment

KW - surface contamination

KW - pathogens

KW - packaging

KW - microorganisms

KW - food technology

KW - diseases

U2 - 10.1109/MODSYM.2004.1433653

DO - 10.1109/MODSYM.2004.1433653

M3 - Paper

ER -

Lamont Y, MacGregor SJ, Anderson JG, Fouracre RA. Effect of visible light exposure on e. coli treated with pulsed UV-rich light. 2004. Paper presented at 26th International Power Modulator Symposium, 2004 and 2004 High-Voltage Workshop , United Kingdom. https://doi.org/10.1109/MODSYM.2004.1433653