Effect of solid-state buffers on the catalytic activity of papain in low water media

T. Theppakorn, P. Kanasawud, P.J. Halling

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The catalytic activity of papain in the synthesis of Z-Gly-Phe- NH2 in tert-butanol has been studied in the presence of solid- state acid-base buffers (acids and their sodium salts). All buffer pairs tested reduced the reaction rate compared with the control, particularly the most acidic and basic (assessed by either aqueous pK(a) or the response of an organic phase indicator). The highest rates, close to the control, were found with glutamic acid/glutamate-Na, PIPES/PIPES-Na and NaH2PO4/Na2HPO4. However, these pairs were unable to erase the pH memory phenomenon, or to overcome the effect of spiking with acetic acid. Hence, at least these buffers do not seem to be able to affect the protonation state and catalytic activity of papain. In the last aqueous solution before drying, the presence of activating agents (cysteine plus EDTA) was more important than buffer ions.
LanguageEnglish
Pages828-836
Number of pages8
JournalEnzyme and Microbial Technology
Volume32
Issue number7
DOIs
Publication statusPublished - 12 Jun 2003

Fingerprint

Papain
Catalyst activity
Buffers
Water
Glutamic Acid
tert-Butyl Alcohol
Acids
Protonation
Edetic Acid
Acetic Acid
Reaction rates
Cysteine
Drying
Salts
Sodium
Ions
Data storage equipment
piperazine-N,N'-bis(2-ethanesulfonic acid)

Keywords

  • papain
  • solid-state buffer
  • catalytic activity
  • low water media Organic-solvents
  • amino-acids
  • esterification
  • biocatalysis
  • ph
  • phase
  • ionizations
  • dipeptides
  • alcohols
  • diols

Cite this

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abstract = "The catalytic activity of papain in the synthesis of Z-Gly-Phe- NH2 in tert-butanol has been studied in the presence of solid- state acid-base buffers (acids and their sodium salts). All buffer pairs tested reduced the reaction rate compared with the control, particularly the most acidic and basic (assessed by either aqueous pK(a) or the response of an organic phase indicator). The highest rates, close to the control, were found with glutamic acid/glutamate-Na, PIPES/PIPES-Na and NaH2PO4/Na2HPO4. However, these pairs were unable to erase the pH memory phenomenon, or to overcome the effect of spiking with acetic acid. Hence, at least these buffers do not seem to be able to affect the protonation state and catalytic activity of papain. In the last aqueous solution before drying, the presence of activating agents (cysteine plus EDTA) was more important than buffer ions.",
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Effect of solid-state buffers on the catalytic activity of papain in low water media. / Theppakorn, T.; Kanasawud, P.; Halling, P.J.

In: Enzyme and Microbial Technology, Vol. 32, No. 7, 12.06.2003, p. 828-836.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of solid-state buffers on the catalytic activity of papain in low water media

AU - Theppakorn, T.

AU - Kanasawud, P.

AU - Halling, P.J.

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N2 - The catalytic activity of papain in the synthesis of Z-Gly-Phe- NH2 in tert-butanol has been studied in the presence of solid- state acid-base buffers (acids and their sodium salts). All buffer pairs tested reduced the reaction rate compared with the control, particularly the most acidic and basic (assessed by either aqueous pK(a) or the response of an organic phase indicator). The highest rates, close to the control, were found with glutamic acid/glutamate-Na, PIPES/PIPES-Na and NaH2PO4/Na2HPO4. However, these pairs were unable to erase the pH memory phenomenon, or to overcome the effect of spiking with acetic acid. Hence, at least these buffers do not seem to be able to affect the protonation state and catalytic activity of papain. In the last aqueous solution before drying, the presence of activating agents (cysteine plus EDTA) was more important than buffer ions.

AB - The catalytic activity of papain in the synthesis of Z-Gly-Phe- NH2 in tert-butanol has been studied in the presence of solid- state acid-base buffers (acids and their sodium salts). All buffer pairs tested reduced the reaction rate compared with the control, particularly the most acidic and basic (assessed by either aqueous pK(a) or the response of an organic phase indicator). The highest rates, close to the control, were found with glutamic acid/glutamate-Na, PIPES/PIPES-Na and NaH2PO4/Na2HPO4. However, these pairs were unable to erase the pH memory phenomenon, or to overcome the effect of spiking with acetic acid. Hence, at least these buffers do not seem to be able to affect the protonation state and catalytic activity of papain. In the last aqueous solution before drying, the presence of activating agents (cysteine plus EDTA) was more important than buffer ions.

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KW - alcohols

KW - diols

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