Abstract
| Language | English |
|---|---|
| Article number | 782 |
| Number of pages | 9 |
| Journal | Frontiers in Microbiology |
| Volume | 8 |
| DOIs | |
| Publication status | Published - 5 May 2017 |
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Keywords
- methicillin-resistant Staphylococcus aureus
- rocket
- Eruca sativa
- proteome
- plant-based media
- bacterial growth
- plant-derived antibacterials
- MRSA
- metabolism
- defense
- extract
Cite this
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Effect of rocket (Eruca sativa) extract on MRSA growth and proteome : metabolic adjustments in plant-based media. / Doulgeraki, Agapi I.; Efthimiou, Georgios; Paramythiotis, Spiros; Pappas, Katherine Maria; Typas, Milton A; Nychas, George-John .
In: Frontiers in Microbiology, Vol. 8, 782, 05.05.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of rocket (Eruca sativa) extract on MRSA growth and proteome
T2 - Frontiers in Microbiology
AU - Doulgeraki, Agapi I.
AU - Efthimiou, Georgios
AU - Paramythiotis, Spiros
AU - Pappas, Katherine Maria
AU - Typas, Milton A
AU - Nychas, George-John
PY - 2017/5/5
Y1 - 2017/5/5
N2 - The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in food has provoked a great concern about the presence of MRSA in associated foodstuff. Although MRSA is often detected in various retailed meat products, it seems that food handlers are more strongly associated with this type of food contamination. Thus, it can be easily postulated that any food could be contaminated with this pathogen in an industrial environment or in household and cause food poisoning. To this direction, the effect of rocket (Eruca sativa) extract on MRSA growth and proteome was examined in the present study. This goal was achieved with the comparative study of the MRSA strain COL proteome, cultivated in rocket extract versus the standard Luria-Bertani growth medium. The obtained results showed that MRSA was able to grow in rocket extract. In addition, proteome analysis using 2-DE method showed that MRSA strain COL is taking advantage of the sugar-, lipid-, and vitamin-rich substrate in the liquid rocket extract, although its growth was delayed in rocket extract compared to Luria– Bertani medium. This work could initiate further research about bacterial metabolism in plant-based media and defense mechanisms against plant-derived antibacterials.
AB - The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in food has provoked a great concern about the presence of MRSA in associated foodstuff. Although MRSA is often detected in various retailed meat products, it seems that food handlers are more strongly associated with this type of food contamination. Thus, it can be easily postulated that any food could be contaminated with this pathogen in an industrial environment or in household and cause food poisoning. To this direction, the effect of rocket (Eruca sativa) extract on MRSA growth and proteome was examined in the present study. This goal was achieved with the comparative study of the MRSA strain COL proteome, cultivated in rocket extract versus the standard Luria-Bertani growth medium. The obtained results showed that MRSA was able to grow in rocket extract. In addition, proteome analysis using 2-DE method showed that MRSA strain COL is taking advantage of the sugar-, lipid-, and vitamin-rich substrate in the liquid rocket extract, although its growth was delayed in rocket extract compared to Luria– Bertani medium. This work could initiate further research about bacterial metabolism in plant-based media and defense mechanisms against plant-derived antibacterials.
KW - methicillin-resistant Staphylococcus aureus
KW - rocket
KW - Eruca sativa
KW - proteome
KW - plant-based media
KW - bacterial growth
KW - plant-derived antibacterials
KW - MRSA
KW - metabolism
KW - defense
KW - extract
U2 - 10.3389/fmicb.2017.00782
DO - 10.3389/fmicb.2017.00782
M3 - Article
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 782
ER -