Effect of phospholipase A on actions of cobra venom cardiotoxins on erythrocytes and skeletal muscle

A L Harvey, R C Hider, F Khader

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The actions of two phospholipase-free cardiotoxins from the venom of the cobra Naja naja siamensis were compared to phospholipase-contaminated cardiotoxins in terms of their ability to lyse human erythrocytes and to depolarize and contract skeletal muscle. The presence of 3-5% (w/w) phospholipase caused a 20-30-fold increase in the haemolytic activity of the two cardiotoxins, the pure cardiotoxins being virtually without haemolytic activity at 10(-7)-10(-6) M. Phospholipase contamination did not enhance the ability of the cardiotoxins to cause contracture of chick biventer cervicis muscles and it caused less than a 2-fold increase in the depolarizing activity of the cardiotoxins on cultured skeletal muscle. Phospholipase-free cardiotoxins were about 10-20 times more active on cultured skeletal muscle fibres than on erythrocytes. These results support the hypothesis that some cardiotoxins have more affinity for the membranes of excitable cells than for those of other cells such as erythrocytes.
LanguageEnglish
Pages215-221
Number of pages7
JournalBBA - Biochimica et Biophysica Acta
Volume728
Issue number2
DOIs
Publication statusPublished - 1983

Fingerprint

Cobra Cardiotoxin Proteins
Cardiotoxins
Cobra Venoms
Phospholipases A
Phospholipases
Skeletal Muscle
Erythrocytes
Elapidae
Skeletal Muscle Fibers
Contracture
Cell Membrane
Muscles

Keywords

  • animals
  • cultured cells
  • chick embryo
  • cobra cardiotoxin proteins
  • cobra venoms
  • drug synergism
  • erythrocytes
  • hemolysis
  • humans
  • membrane potentials
  • muscle contraction
  • muscles
  • phospholipases
  • phospholipases A

Cite this

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title = "Effect of phospholipase A on actions of cobra venom cardiotoxins on erythrocytes and skeletal muscle",
abstract = "The actions of two phospholipase-free cardiotoxins from the venom of the cobra Naja naja siamensis were compared to phospholipase-contaminated cardiotoxins in terms of their ability to lyse human erythrocytes and to depolarize and contract skeletal muscle. The presence of 3-5{\%} (w/w) phospholipase caused a 20-30-fold increase in the haemolytic activity of the two cardiotoxins, the pure cardiotoxins being virtually without haemolytic activity at 10(-7)-10(-6) M. Phospholipase contamination did not enhance the ability of the cardiotoxins to cause contracture of chick biventer cervicis muscles and it caused less than a 2-fold increase in the depolarizing activity of the cardiotoxins on cultured skeletal muscle. Phospholipase-free cardiotoxins were about 10-20 times more active on cultured skeletal muscle fibres than on erythrocytes. These results support the hypothesis that some cardiotoxins have more affinity for the membranes of excitable cells than for those of other cells such as erythrocytes.",
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Effect of phospholipase A on actions of cobra venom cardiotoxins on erythrocytes and skeletal muscle. / Harvey, A L; Hider, R C; Khader, F.

In: BBA - Biochimica et Biophysica Acta, Vol. 728, No. 2, 1983, p. 215-221.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of phospholipase A on actions of cobra venom cardiotoxins on erythrocytes and skeletal muscle

AU - Harvey, A L

AU - Hider, R C

AU - Khader, F

PY - 1983

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N2 - The actions of two phospholipase-free cardiotoxins from the venom of the cobra Naja naja siamensis were compared to phospholipase-contaminated cardiotoxins in terms of their ability to lyse human erythrocytes and to depolarize and contract skeletal muscle. The presence of 3-5% (w/w) phospholipase caused a 20-30-fold increase in the haemolytic activity of the two cardiotoxins, the pure cardiotoxins being virtually without haemolytic activity at 10(-7)-10(-6) M. Phospholipase contamination did not enhance the ability of the cardiotoxins to cause contracture of chick biventer cervicis muscles and it caused less than a 2-fold increase in the depolarizing activity of the cardiotoxins on cultured skeletal muscle. Phospholipase-free cardiotoxins were about 10-20 times more active on cultured skeletal muscle fibres than on erythrocytes. These results support the hypothesis that some cardiotoxins have more affinity for the membranes of excitable cells than for those of other cells such as erythrocytes.

AB - The actions of two phospholipase-free cardiotoxins from the venom of the cobra Naja naja siamensis were compared to phospholipase-contaminated cardiotoxins in terms of their ability to lyse human erythrocytes and to depolarize and contract skeletal muscle. The presence of 3-5% (w/w) phospholipase caused a 20-30-fold increase in the haemolytic activity of the two cardiotoxins, the pure cardiotoxins being virtually without haemolytic activity at 10(-7)-10(-6) M. Phospholipase contamination did not enhance the ability of the cardiotoxins to cause contracture of chick biventer cervicis muscles and it caused less than a 2-fold increase in the depolarizing activity of the cardiotoxins on cultured skeletal muscle. Phospholipase-free cardiotoxins were about 10-20 times more active on cultured skeletal muscle fibres than on erythrocytes. These results support the hypothesis that some cardiotoxins have more affinity for the membranes of excitable cells than for those of other cells such as erythrocytes.

KW - animals

KW - cultured cells

KW - chick embryo

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KW - hemolysis

KW - humans

KW - membrane potentials

KW - muscle contraction

KW - muscles

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KW - phospholipases A

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