Effect of extracellular polymeric substances on the mechanical properties of Rhodococcus

Yu Pen, Zhenyu J. Zhang, Ana L. Morales-García, Matthew Mears, Drew S. Tarmey, Robert G. Edyvean, Steven A. Banwart, Mark Geoghegan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The mechanical properties of Rhodococcus RC291 were measured using force spectroscopy equipped with a bacterial cell probe. Rhodococcal cells in the late growth stage of development were found to have greater adhesion to a silicon oxide surface than those in the early growth stage. This is because there are more extracellular polymeric substances (EPS) that contain nonspecific binding sites available on the cells of late growth stage. It is found that EPS in the late exponential phase are less densely bound but consist of chains able to extend further into their local environment, whilst the denser EPS at the late stationary phase act more to sheath the cell. Contraction and extension of the EPS could change the density of the binding sites, and therefore affect the magnitude of the adhesion force between the EPS and the silicon oxide surface. By treating rhodococcal EPS as a surface-grafted polyelectrolyte layer and using scaling theory, the interaction between EPS and a solid substrate was modelled for the cell approaching the surface which revealed that EPS possess a large capacity to store charge. Changing the pH of the surrounding medium acts to change the conformation of EPS chains.
LanguageEnglish
Pages518–526
Number of pages9
JournalBBA - Biomembranes
Volume1848
Issue number2
Early online date22 Nov 2014
DOIs
Publication statusPublished - Feb 2015

Fingerprint

Rhodococcus
Mechanical properties
Silicon oxides
Silicon
Adhesion
Oxides
Binding Sites
Polyelectrolytes
Conformations
Growth
Growth and Development
Spectroscopy
Spectrum Analysis
Substrates

Keywords

  • extracellular polymeric substances
  • Rhodococcus RC291
  • silicon oxide surfaces

Cite this

Pen, Y., Zhang, Z. J., Morales-García, A. L., Mears, M., Tarmey, D. S., Edyvean, R. G., ... Geoghegan, M. (2015). Effect of extracellular polymeric substances on the mechanical properties of Rhodococcus. BBA - Biomembranes, 1848(2), 518–526. https://doi.org/10.1016/j.bbamem.2014.11.007
Pen, Yu ; Zhang, Zhenyu J. ; Morales-García, Ana L. ; Mears, Matthew ; Tarmey, Drew S. ; Edyvean, Robert G. ; Banwart, Steven A. ; Geoghegan, Mark. / Effect of extracellular polymeric substances on the mechanical properties of Rhodococcus. In: BBA - Biomembranes. 2015 ; Vol. 1848, No. 2. pp. 518–526.
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abstract = "The mechanical properties of Rhodococcus RC291 were measured using force spectroscopy equipped with a bacterial cell probe. Rhodococcal cells in the late growth stage of development were found to have greater adhesion to a silicon oxide surface than those in the early growth stage. This is because there are more extracellular polymeric substances (EPS) that contain nonspecific binding sites available on the cells of late growth stage. It is found that EPS in the late exponential phase are less densely bound but consist of chains able to extend further into their local environment, whilst the denser EPS at the late stationary phase act more to sheath the cell. Contraction and extension of the EPS could change the density of the binding sites, and therefore affect the magnitude of the adhesion force between the EPS and the silicon oxide surface. By treating rhodococcal EPS as a surface-grafted polyelectrolyte layer and using scaling theory, the interaction between EPS and a solid substrate was modelled for the cell approaching the surface which revealed that EPS possess a large capacity to store charge. Changing the pH of the surrounding medium acts to change the conformation of EPS chains.",
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Pen, Y, Zhang, ZJ, Morales-García, AL, Mears, M, Tarmey, DS, Edyvean, RG, Banwart, SA & Geoghegan, M 2015, 'Effect of extracellular polymeric substances on the mechanical properties of Rhodococcus' BBA - Biomembranes, vol. 1848, no. 2, pp. 518–526. https://doi.org/10.1016/j.bbamem.2014.11.007

Effect of extracellular polymeric substances on the mechanical properties of Rhodococcus. / Pen, Yu; Zhang, Zhenyu J.; Morales-García, Ana L.; Mears, Matthew; Tarmey, Drew S.; Edyvean, Robert G.; Banwart, Steven A. ; Geoghegan, Mark.

In: BBA - Biomembranes, Vol. 1848, No. 2, 02.2015, p. 518–526.

Research output: Contribution to journalArticle

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AU - Pen, Yu

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AU - Morales-García, Ana L.

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AB - The mechanical properties of Rhodococcus RC291 were measured using force spectroscopy equipped with a bacterial cell probe. Rhodococcal cells in the late growth stage of development were found to have greater adhesion to a silicon oxide surface than those in the early growth stage. This is because there are more extracellular polymeric substances (EPS) that contain nonspecific binding sites available on the cells of late growth stage. It is found that EPS in the late exponential phase are less densely bound but consist of chains able to extend further into their local environment, whilst the denser EPS at the late stationary phase act more to sheath the cell. Contraction and extension of the EPS could change the density of the binding sites, and therefore affect the magnitude of the adhesion force between the EPS and the silicon oxide surface. By treating rhodococcal EPS as a surface-grafted polyelectrolyte layer and using scaling theory, the interaction between EPS and a solid substrate was modelled for the cell approaching the surface which revealed that EPS possess a large capacity to store charge. Changing the pH of the surrounding medium acts to change the conformation of EPS chains.

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Pen Y, Zhang ZJ, Morales-García AL, Mears M, Tarmey DS, Edyvean RG et al. Effect of extracellular polymeric substances on the mechanical properties of Rhodococcus. BBA - Biomembranes. 2015 Feb;1848(2):518–526. https://doi.org/10.1016/j.bbamem.2014.11.007