Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS

Jonans Tusiimire, Jennifer Wallace, Nicola Woods, Mark J. Dufton, John A. Parkinson, Grainne Abbott, Carol J. Clements, Louise Young, Jin Kyu Park, Jong Woon Jeon, Valerie A. Ferro, David G. Watson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The venom of Apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. Four fractions from whole bee venom (BV) were separated using medium pressure liquid chromatography. Their ability to induce the production of cytokines TNFα, IL-1β and IL-6 in phorbol-12-myristate-13-acetate (PMA)-treated U937 cells was assessed. The levels of the three cytokines produced by stimulation with the four fractions and crude BV without LPS were not significantly different from negative control values. However, co-stimulation of the cells with LPS and Fraction 4 (F-4) induced a 1.6-fold increase in TNF-α level (p < 0.05) compared to LPS alone. Likewise, LPS-induced IL-1β production was significantly synergised in the presence of F-1 (nine-fold), F-2 (six-fold), F-3 (four-fold) and F-4 (two-fold) fractions, but was only slightly enhanced with crude BV (1.5-fold) relative to LPS. Furthermore, the LPS-stimulated production of IL-6 was not significantly increased in cells co-treated with F-2 and F-3, but the organic fraction (F-4) showed an inhibitory effect (p < 0.05) on IL-6 production. The latter was elucidated by NMR spectroscopy and found to contain(Z)-9-eicosen-1-ol. The effects observed with the purified BV fractions were more marked than those obtained with the crude sample.

LanguageEnglish
Article number11
Number of pages15
JournalVaccines
Volume4
Issue number2
DOIs
Publication statusPublished - 19 Apr 2016

Fingerprint

Bee Venoms
U937 Cells
Acetates
Cytokines
Interleukin-6
Honey
Bees
Liquid Chromatography
Immunotherapy
Magnetic Resonance Spectroscopy
Tumor Necrosis Factor-alpha
phorbol-12-myristate
Pressure
F 4

Keywords

  • bee venom
  • pro-inflammatory cytokines
  • LPS stimulation
  • U937 cells
  • PMA differentiated
  • Apis melllifera

Cite this

@article{c616961dc8d749d6a404b1a924454004,
title = "Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS",
abstract = "The venom of Apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. Four fractions from whole bee venom (BV) were separated using medium pressure liquid chromatography. Their ability to induce the production of cytokines TNFα, IL-1β and IL-6 in phorbol-12-myristate-13-acetate (PMA)-treated U937 cells was assessed. The levels of the three cytokines produced by stimulation with the four fractions and crude BV without LPS were not significantly different from negative control values. However, co-stimulation of the cells with LPS and Fraction 4 (F-4) induced a 1.6-fold increase in TNF-α level (p < 0.05) compared to LPS alone. Likewise, LPS-induced IL-1β production was significantly synergised in the presence of F-1 (nine-fold), F-2 (six-fold), F-3 (four-fold) and F-4 (two-fold) fractions, but was only slightly enhanced with crude BV (1.5-fold) relative to LPS. Furthermore, the LPS-stimulated production of IL-6 was not significantly increased in cells co-treated with F-2 and F-3, but the organic fraction (F-4) showed an inhibitory effect (p < 0.05) on IL-6 production. The latter was elucidated by NMR spectroscopy and found to contain(Z)-9-eicosen-1-ol. The effects observed with the purified BV fractions were more marked than those obtained with the crude sample.",
keywords = "bee venom, pro-inflammatory cytokines, LPS stimulation, U937 cells, PMA differentiated, Apis melllifera",
author = "Jonans Tusiimire and Jennifer Wallace and Nicola Woods and Dufton, {Mark J.} and Parkinson, {John A.} and Grainne Abbott and Clements, {Carol J.} and Louise Young and Park, {Jin Kyu} and Jeon, {Jong Woon} and Ferro, {Valerie A.} and Watson, {David G.}",
year = "2016",
month = "4",
day = "19",
doi = "10.3390/vaccines4020011",
language = "English",
volume = "4",
journal = "Vaccines",
issn = "2076-393X",
number = "2",

}

Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS. / Tusiimire, Jonans; Wallace, Jennifer; Woods, Nicola; Dufton, Mark J.; Parkinson, John A.; Abbott, Grainne; Clements, Carol J.; Young, Louise; Park, Jin Kyu; Jeon, Jong Woon; Ferro, Valerie A.; Watson, David G.

In: Vaccines, Vol. 4, No. 2, 11, 19.04.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS

AU - Tusiimire, Jonans

AU - Wallace, Jennifer

AU - Woods, Nicola

AU - Dufton, Mark J.

AU - Parkinson, John A.

AU - Abbott, Grainne

AU - Clements, Carol J.

AU - Young, Louise

AU - Park, Jin Kyu

AU - Jeon, Jong Woon

AU - Ferro, Valerie A.

AU - Watson, David G.

PY - 2016/4/19

Y1 - 2016/4/19

N2 - The venom of Apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. Four fractions from whole bee venom (BV) were separated using medium pressure liquid chromatography. Their ability to induce the production of cytokines TNFα, IL-1β and IL-6 in phorbol-12-myristate-13-acetate (PMA)-treated U937 cells was assessed. The levels of the three cytokines produced by stimulation with the four fractions and crude BV without LPS were not significantly different from negative control values. However, co-stimulation of the cells with LPS and Fraction 4 (F-4) induced a 1.6-fold increase in TNF-α level (p < 0.05) compared to LPS alone. Likewise, LPS-induced IL-1β production was significantly synergised in the presence of F-1 (nine-fold), F-2 (six-fold), F-3 (four-fold) and F-4 (two-fold) fractions, but was only slightly enhanced with crude BV (1.5-fold) relative to LPS. Furthermore, the LPS-stimulated production of IL-6 was not significantly increased in cells co-treated with F-2 and F-3, but the organic fraction (F-4) showed an inhibitory effect (p < 0.05) on IL-6 production. The latter was elucidated by NMR spectroscopy and found to contain(Z)-9-eicosen-1-ol. The effects observed with the purified BV fractions were more marked than those obtained with the crude sample.

AB - The venom of Apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. Four fractions from whole bee venom (BV) were separated using medium pressure liquid chromatography. Their ability to induce the production of cytokines TNFα, IL-1β and IL-6 in phorbol-12-myristate-13-acetate (PMA)-treated U937 cells was assessed. The levels of the three cytokines produced by stimulation with the four fractions and crude BV without LPS were not significantly different from negative control values. However, co-stimulation of the cells with LPS and Fraction 4 (F-4) induced a 1.6-fold increase in TNF-α level (p < 0.05) compared to LPS alone. Likewise, LPS-induced IL-1β production was significantly synergised in the presence of F-1 (nine-fold), F-2 (six-fold), F-3 (four-fold) and F-4 (two-fold) fractions, but was only slightly enhanced with crude BV (1.5-fold) relative to LPS. Furthermore, the LPS-stimulated production of IL-6 was not significantly increased in cells co-treated with F-2 and F-3, but the organic fraction (F-4) showed an inhibitory effect (p < 0.05) on IL-6 production. The latter was elucidated by NMR spectroscopy and found to contain(Z)-9-eicosen-1-ol. The effects observed with the purified BV fractions were more marked than those obtained with the crude sample.

KW - bee venom

KW - pro-inflammatory cytokines

KW - LPS stimulation

KW - U937 cells

KW - PMA differentiated

KW - Apis melllifera

UR - http://www.mdpi.com/2076-393X/4/2/11

U2 - 10.3390/vaccines4020011

DO - 10.3390/vaccines4020011

M3 - Article

VL - 4

JO - Vaccines

T2 - Vaccines

JF - Vaccines

SN - 2076-393X

IS - 2

M1 - 11

ER -