Effect of 405 nm high-intensity narrow-spectrum light on fibroblast populated collagen lattices: an in vitro model of wound healing

Richard Mcdonald, M. Helen Grant, Scott J. Macgregor, John G. Anderson, Michelle Maclean

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22 Citations (Scopus)

Abstract

High-intensity narrow-spectrum (HINS) 405-nm light is a novel technology developed to address the significant problem of health-care associated infection. Its potential for wound-decontamination applications is assessed on mammalian cells and bacteria. The fibroblast-populated collagen lattice (FPCL) is used as an in vitro model of wound healing, and the effect of HINS light on contraction is examined. Effects on cell proliferation, morphological changes, and α-smooth muscle actin (α-SMA) expression are investigated. Bactericidal effects are assessed using the bacterium Staphylococcus epidermidis. Low doses of HINS light were found to have no significant inhibitory effects on FPCL contraction, cell proliferation, or α-SMA expression. Doses of up to 18 Jcm−2 had no significant inhibitory effects on FPCL cell numbers, and this dose was shown to cause almost complete inactivation of bacteria. These results show that HINS light has potential for disinfection applications without adversely influencing wound healing.
LanguageEnglish
Article number048003
Number of pages4
JournalJournal of Biomedical Optics
Volume16
Issue number4
Early online date30 Apr 2011
DOIs
Publication statusPublished - Apr 2011

Fingerprint

wound healing
fibroblasts
Fibroblasts
collagens
Collagen
Bacteria
bacteria
Cell proliferation
smooth muscle
Muscle
Actins
dosage
contraction
Decontamination
Disinfection
Bioelectric potentials
decontamination
Health care
staphylococcus
Dosimetry

Keywords

  • bio-optics
  • cellular biophysics
  • decontamination
  • microorganisms
  • radiation therapy

Cite this

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abstract = "High-intensity narrow-spectrum (HINS) 405-nm light is a novel technology developed to address the significant problem of health-care associated infection. Its potential for wound-decontamination applications is assessed on mammalian cells and bacteria. The fibroblast-populated collagen lattice (FPCL) is used as an in vitro model of wound healing, and the effect of HINS light on contraction is examined. Effects on cell proliferation, morphological changes, and α-smooth muscle actin (α-SMA) expression are investigated. Bactericidal effects are assessed using the bacterium Staphylococcus epidermidis. Low doses of HINS light were found to have no significant inhibitory effects on FPCL contraction, cell proliferation, or α-SMA expression. Doses of up to 18 Jcm−2 had no significant inhibitory effects on FPCL cell numbers, and this dose was shown to cause almost complete inactivation of bacteria. These results show that HINS light has potential for disinfection applications without adversely influencing wound healing.",
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