Dynamic imaging analysis of SERS-active nanoparticle clusters in suspension

A.W. Wark, Robert Stokes, Steven B. Darby, W.E. Smith, D. Graham

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A novel wide-field approach for the real-time Surface Enhanced Raman Scattering (SERS) imaging of multiple silver nanoparticle clusters suspended in solution is described. This method enables direct correlation of the SERS activity of a single nanoparticle aggregate and its size through measurement of the cluster diffusion coefficient and can also be performed in a high-throughput basis. As a first demonstration, we investigate the salt-induced aggregation of silver nanoparticles in the presence of a reporter tag molecule which has a high affinity for the nanoparticle surface. In addition to tracking individual particles, direct comparison of Rayleigh and SERS videos of the same colloid solution enabled measurement of the fraction of individual clusters that are SERS active and the dependence of this value on the relative concentration of the tag molecule. Furthermore, given the ability to also rapidly profile any non-uniformity in particle size distributions, we expect this approach will not only provide a new tool for the fundamental understanding of SERS but also significantly contribute to the development of an array of emerging nanoparticle-enhanced biomolecule and imaging detection platforms.
LanguageEnglish
Pages18115-18120
Number of pages6
JournalJournal of Physical Chemistry C
Volume114
Issue number42
DOIs
Publication statusPublished - 5 Oct 2010

Fingerprint

Raman scattering
Suspensions
Raman spectra
Nanoparticles
Imaging techniques
nanoparticles
Silver
silver
Molecules
Colloids
Biomolecules
particle size distribution
Particle size analysis
nonuniformity
colloids
affinity
molecules
emerging
Demonstrations
Agglomeration

Keywords

  • surface-enhanced-raman
  • single-particle tracking
  • scattering
  • spectroscopy
  • silver
  • dimers
  • trimers
  • point
  • dyes
  • dna

Cite this

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title = "Dynamic imaging analysis of SERS-active nanoparticle clusters in suspension",
abstract = "A novel wide-field approach for the real-time Surface Enhanced Raman Scattering (SERS) imaging of multiple silver nanoparticle clusters suspended in solution is described. This method enables direct correlation of the SERS activity of a single nanoparticle aggregate and its size through measurement of the cluster diffusion coefficient and can also be performed in a high-throughput basis. As a first demonstration, we investigate the salt-induced aggregation of silver nanoparticles in the presence of a reporter tag molecule which has a high affinity for the nanoparticle surface. In addition to tracking individual particles, direct comparison of Rayleigh and SERS videos of the same colloid solution enabled measurement of the fraction of individual clusters that are SERS active and the dependence of this value on the relative concentration of the tag molecule. Furthermore, given the ability to also rapidly profile any non-uniformity in particle size distributions, we expect this approach will not only provide a new tool for the fundamental understanding of SERS but also significantly contribute to the development of an array of emerging nanoparticle-enhanced biomolecule and imaging detection platforms.",
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Dynamic imaging analysis of SERS-active nanoparticle clusters in suspension. / Wark, A.W.; Stokes, Robert; Darby, Steven B.; Smith, W.E.; Graham, D.

In: Journal of Physical Chemistry C, Vol. 114, No. 42, 05.10.2010, p. 18115-18120.

Research output: Contribution to journalArticle

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AU - Wark, A.W.

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AU - Graham, D.

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