Abstract
A novel dual nanoparticle amplification approach is introduced for the enhanced surface plasmon resonance (SPR) detection of a target protein at subattomolar concentrations. Thrombin was used as a model target protein as part of a sandwich assay involving an antithrombin (anti-Th) modified SPR chip surface and a thrombin specific DNA aptamer (Th-aptamer) whose sequence also includes a polyadenine (A30) tail. Dual nanoparticle (NP) enhancement was achieved with the controlled hybridization adsorption of first polythymine-NP conjugates (T20-NPs) followed by polyadenine-NPs (A30-NPs). Two different nanoparticle shapes (nanorod and quasi-spherical) were explored resulting in four different NP pair combinations being directly compared. It was found that both the order and NP shape were important in optimizing the assay performance. The use of real-time SPR measurements to detect target concentrations as low as 0.1 aM is a 10-fold improvement compared to single NP-enhanced SPR detection methods.
| Language | English |
|---|---|
| Pages | 9824-9829 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 86 |
| Issue number | 19 |
| Early online date | 4 Sep 2014 |
| DOIs | |
| Publication status | Published - 7 Oct 2014 |
Fingerprint
Keywords
- dual nanoparticle amplification
- protein
- assay performance
- surface plasmon resonance (SPR)
Cite this
}
Dual nanoparticle amplified surface plasmon resonance detection of thrombin at subattomolar concentrations. / Baek, Seung Hee; Wark, Alastair W.; Lee, Hye Jin.
In: Analytical Chemistry, Vol. 86, No. 19, 07.10.2014, p. 9824-9829.Research output: Contribution to journal › Article
TY - JOUR
T1 - Dual nanoparticle amplified surface plasmon resonance detection of thrombin at subattomolar concentrations
AU - Baek, Seung Hee
AU - Wark, Alastair W.
AU - Lee, Hye Jin
PY - 2014/10/7
Y1 - 2014/10/7
N2 - A novel dual nanoparticle amplification approach is introduced for the enhanced surface plasmon resonance (SPR) detection of a target protein at subattomolar concentrations. Thrombin was used as a model target protein as part of a sandwich assay involving an antithrombin (anti-Th) modified SPR chip surface and a thrombin specific DNA aptamer (Th-aptamer) whose sequence also includes a polyadenine (A30) tail. Dual nanoparticle (NP) enhancement was achieved with the controlled hybridization adsorption of first polythymine-NP conjugates (T20-NPs) followed by polyadenine-NPs (A30-NPs). Two different nanoparticle shapes (nanorod and quasi-spherical) were explored resulting in four different NP pair combinations being directly compared. It was found that both the order and NP shape were important in optimizing the assay performance. The use of real-time SPR measurements to detect target concentrations as low as 0.1 aM is a 10-fold improvement compared to single NP-enhanced SPR detection methods.
AB - A novel dual nanoparticle amplification approach is introduced for the enhanced surface plasmon resonance (SPR) detection of a target protein at subattomolar concentrations. Thrombin was used as a model target protein as part of a sandwich assay involving an antithrombin (anti-Th) modified SPR chip surface and a thrombin specific DNA aptamer (Th-aptamer) whose sequence also includes a polyadenine (A30) tail. Dual nanoparticle (NP) enhancement was achieved with the controlled hybridization adsorption of first polythymine-NP conjugates (T20-NPs) followed by polyadenine-NPs (A30-NPs). Two different nanoparticle shapes (nanorod and quasi-spherical) were explored resulting in four different NP pair combinations being directly compared. It was found that both the order and NP shape were important in optimizing the assay performance. The use of real-time SPR measurements to detect target concentrations as low as 0.1 aM is a 10-fold improvement compared to single NP-enhanced SPR detection methods.
KW - dual nanoparticle amplification
KW - protein
KW - assay performance
KW - surface plasmon resonance (SPR)
UR - http://www.scopus.com/inward/record.url?scp=84907938356&partnerID=8YFLogxK
U2 - 10.1021/ac5024183
DO - 10.1021/ac5024183
M3 - Article
VL - 86
SP - 9824
EP - 9829
JO - Analytical Chemistry
T2 - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 19
ER -