Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity

Enitome E. Bafor, Wellington O. Omokaro, Osamuyi H. Uwumarongie, Uloma B. Elvis-Offiah, Osemelomen Omoruyi, RuAngelie Edrada-Ebel, Christina V. Viegelmann

Research output: Contribution to journalArticle

Abstract

Background: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects.
Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro.Setting: Fresh leaves of D. filix-mas were collected from a river bank in the south-western part of Nigeria.Methods: The leaves of D. filix-mas were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites.Results: The plant extract inhibited spontaneous-induced contractions with IC50 amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC50 frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions (p < 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified.Conclusion: The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect.
LanguageEnglish
Article numbera25
Number of pages12
JournalJournal of Medicinal Plants For Economic Development
Volume1
Issue number1
DOIs
Publication statusPublished - 25 Oct 2017

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Dryopteridaceae
Dryopteris
Oxytocin
Uterine Contraction
Plant Extracts
Contraceptive Agents
Calcium
Inhibitory Concentration 50
Limonins
Oxytocics
Tocolytic Agents
Terpenes
Saponins
Porphyrins
Nigeria
Glycosides
Alkaloids
Rivers
Uterus
Methanol

Keywords

  • Dryopteris filix-mas
  • porphyrins
  • fatty acids
  • oxytocin
  • uterus

Cite this

Bafor, E. E., Omokaro, W. O., Uwumarongie, O. H., Elvis-Offiah, U. B., Omoruyi, O., Edrada-Ebel, R., & Viegelmann, C. V. (2017). Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity. Journal of Medicinal Plants For Economic Development, 1(1), [a25]. https://doi.org/10.4102/jomped.v1i1.25
Bafor, Enitome E. ; Omokaro, Wellington O. ; Uwumarongie, Osamuyi H. ; Elvis-Offiah, Uloma B. ; Omoruyi, Osemelomen ; Edrada-Ebel, RuAngelie ; Viegelmann, Christina V. / Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity. In: Journal of Medicinal Plants For Economic Development. 2017 ; Vol. 1, No. 1.
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Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity. / Bafor, Enitome E.; Omokaro, Wellington O.; Uwumarongie, Osamuyi H.; Elvis-Offiah, Uloma B.; Omoruyi, Osemelomen; Edrada-Ebel, RuAngelie; Viegelmann, Christina V.

In: Journal of Medicinal Plants For Economic Development, Vol. 1, No. 1, a25, 25.10.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity

AU - Bafor, Enitome E.

AU - Omokaro, Wellington O.

AU - Uwumarongie, Osamuyi H.

AU - Elvis-Offiah, Uloma B.

AU - Omoruyi, Osemelomen

AU - Edrada-Ebel, RuAngelie

AU - Viegelmann, Christina V.

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Y1 - 2017/10/25

N2 - Background: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects. Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro.Setting: Fresh leaves of D. filix-mas were collected from a river bank in the south-western part of Nigeria.Methods: The leaves of D. filix-mas were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites.Results: The plant extract inhibited spontaneous-induced contractions with IC50 amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC50 frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions (p < 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified.Conclusion: The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect.

AB - Background: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects. Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro.Setting: Fresh leaves of D. filix-mas were collected from a river bank in the south-western part of Nigeria.Methods: The leaves of D. filix-mas were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites.Results: The plant extract inhibited spontaneous-induced contractions with IC50 amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC50 frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions (p < 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified.Conclusion: The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect.

KW - Dryopteris filix-mas

KW - porphyrins

KW - fatty acids

KW - oxytocin

KW - uterus

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